2. Stem Cell/Wnt Metabolic Enzyme/Protease
  3. Smo Endogenous Metabolite
  4. 20(S)-Hydroxycholesterol

20(S)-Hydroxycholesterol  (Synonyms: 20α-Hydroxycholesterol)

Cat. No.: HY-12316 Purity: 98.70%
COA Handling Instructions

20(S)-hydroxyCholesterol (20α-Hydroxycholesterol) is an allosteric activator of the oncoprotein smoothened (Smo) that activates the hedgehog (Hh) signaling pathway with an EC50 of 3 μM in a gene transcription reporter assay using NIH3T3 cells.

For research use only. We do not sell to patients.

20(S)-Hydroxycholesterol Chemical Structure

20(S)-Hydroxycholesterol Chemical Structure

CAS No. : 516-72-3

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Solid + Solvent
10 mM * 1 mL in DMSO
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Solid
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Based on 1 publication(s) in Google Scholar

20(S)-Hydroxycholesterol Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE 20(S)-Hydroxycholesterol

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Description

20(S)-hydroxyCholesterol (20α-Hydroxycholesterol) is an allosteric activator of the oncoprotein smoothened (Smo) that activates the hedgehog (Hh) signaling pathway with an EC50 of 3 μM in a gene transcription reporter assay using NIH3T3 cells[1][2].

IC50 & Target

Human Endogenous Metabolite

 

Molecular Weight

402.65

Appearance

Solid

Formula

C27H46O2
CAS No.
SMILES

C[C@@]1([C@@]2([H])[C@@](C)(O)CCCC(C)C)[C@](CC2)([H])[C@@](CC=C3[C@@]4(CC[C@H](O)C3)C)([H])[C@]4([H])CC1
Structure Classification
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 30 mg/mL (74.51 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4835 mL 12.4177 mL 24.8355 mL
5 mM 0.4967 mL 2.4835 mL 4.9671 mL
10 mM 0.2484 mL 1.2418 mL 2.4835 mL
*Please refer to the solubility information to select the appropriate solvent.
Purity & Documentation

Purity: 98.70%

COA (247 KB) RP-HPLC (260 KB)

Handling Instructions (2659 KB)
References
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20(S)-Hydroxycholesterol Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

20(S)-Hydroxycholesterol516-72-320α-HydroxycholesterolSmoEndogenous MetaboliteSmoothenedInhibitorinhibitorinhibit

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