24-Epicastasterone
Based on 2 publication(s) in Google Scholar
24-Epicastasterone (24-epi-Castasterone) is a bioactive brassinosteroid and a ligand of ABCB1 and ABCB19 in Arabidopsis thaliana. 24-Epicastasterone stimulates the ATPase activity of ABCB19 and the ATP hydrolysis activity of ABCB1, which drive the efflux of substances from plant cells. 24-Epicastasterone increases the activities of catalase and guaiacol peroxidase in the roots of wheat seedlings. 24-Epicastasterone alleviates heat-induced lipid peroxidation through a ROS-dependent mechanism and enhances the heat tolerance of common wheat seedlings.
For research use only. We do not sell to patients.
- Purity: 99.91%
- CAS No.: 72050-71-6
- Formula: C28H48O5
- Molecular Weight:464.68
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 24-Epicastasterone
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Biological Activity
24-epicastasterone (20 min pre-incubation on ice, 10 min reaction at 37°C) potently stimulates the ATPase activity of purified Arabidopsis ABCB19 protein in a dose-dependent manner[1].
24-epicastasterone (0.05 mM) binds directly to purified Arabidopsis ABCB19 protein reconstituted in amphipols with a dissociation constant of 0.36 μM[1].
24-Epicastasterone (1 μM; foliar spray; once daily; 11 consecutive days) rescues growth in brassinosteroid-deficient Arabidopsis biosynthetic mutants but not in ABCB19 transporter-deficient mutants, confirming ABCB19 is required for functional 24-epicastasterone signaling[1].
24-epicastasterone (5-200 nM; 24 h) increases the heat resistance of Triticum aestivum cv. Doskonala wheat seedlings, with the most significant effect observed at a 50 nM concentration applied for 24 h, and a significant effect also seen at 20 nM[2].
24-epicastasterone (50 nM; 2 h, 4 h, 24 h) modulates the activity of superoxide dismutase, catalase, and guaiacol peroxidase in roots of Triticum aestivum cv. Doskonala wheat seedlings, with superoxide dismutase activity elevated at 4 h, catalase activity elevated at 24 h, and guaiacol peroxidase activity elevated at 4 h but not significantly different from control at 24 h[2].
24-epicastasterone (50 nM; 24 h before heat stress) reduces heat-induced lipid peroxidation in roots of Triticum aestivum cv. Doskonala wheat seedlings, an effect dependent on NADPH oxidase-mediated hydrogen peroxide production[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 72050-71-6
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Appearance Solid
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Molecular Weight 464.68
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Formula C28H48O5
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Color Off-white to light brown
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SMILES
C[C@@]12[C@]3([H])[C@](CC([C@@]1([H])C[C@@H]([C@@H](C2)O)O)=O)([H])[C@@]4([H])[C@](CC3)([C@@](CC4)([H])[C@H](C)[C@@H](O)[C@H](O)[C@H](C)C(C)C)C
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Synonyms
24-epi-Castasterone
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Initial Source
Hydrodictyon reticulatum
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (2)
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Journal Impact Factor
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Most Recent
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Science
Structure and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid export. [Abstract]2024 Mar 22;383(6689):eadj4591. PMID: 38513023 -
Plant Commun
Structural insights into brassinosteroid export mediated by the Arabidopsis ABC transporter ABCB1. [Abstract]2025 Jan 13;6(1):101181. PMID: 39497419
Solvent & Solubility
THF : 10 mg/mL (21.52 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Ying W, et al. Structure and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid export. Science. 2024 Mar 22;383(6689):eadj4591. [Content Brief]
[3]. Wei H, et al. Structural insights into brassinosteroid export mediated by the Arabidopsis ABC transporter ABCB1. Plant Commun. 2025;6(1):101181. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| THF | 1 mM | 2.1520 mL | 10.7601 mL | 21.5202 mL | 53.8005 mL |
| 5 mM | 0.4304 mL | 2.1520 mL | 4.3040 mL | 10.7601 mL | |
| 10 mM | 0.2152 mL | 1.0760 mL | 2.1520 mL | 5.3800 mL | |
| 15 mM | 0.1435 mL | 0.7173 mL | 1.4347 mL | 3.5867 mL | |
| 20 mM | 0.1076 mL | 0.5380 mL | 1.0760 mL | 2.6900 mL |
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