3,4-Dimethoxychalcone
Based on 1 publication(s) in Google Scholar
3,4-Dimethoxychalcone is a Caloric restriction mimetics (CRMs). 3,4-Dimethoxychalcone induces the deacetylation of cytoplasmic proteins and stimulates autophagy flux. 3,4-Dimethoxychalcone can be used for cardiac and cancer diseases research.
For research use only. We do not sell to patients.
- Purity: 99.94%
- CAS No.: 5416-71-7
- Formula: C17H16O3
- Molecular Weight:268.31
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) 3,4-Dimethoxychalcone
More
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Neutrophil | IC50 |
15 μM
Compound: 19
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Inhibition of PMA-induce ROS/RNS generation in human neutrophils measured up to 30 mins in presence of 5.5 mM glucose by luminol-amplified chemiluminescence method
Inhibition of PMA-induce ROS/RNS generation in human neutrophils measured up to 30 mins in presence of 5.5 mM glucose by luminol-amplified chemiluminescence method
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[PMID: 33006891] |
| Neutrophil | IC50 |
20 μM
Compound: 19
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Inhibition of PMA-induce ROS/RNS generation in human neutrophils measured up to 30 mins in presence of 30 mM glucose by luminol-amplified chemiluminescence method
Inhibition of PMA-induce ROS/RNS generation in human neutrophils measured up to 30 mins in presence of 30 mM glucose by luminol-amplified chemiluminescence method
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[PMID: 33006891] |
| RBL-1 | IC50 |
18 μM
Compound: 38
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Inhibition of 5-lipoxygenase in rat RBL1 cells
Inhibition of 5-lipoxygenase in rat RBL1 cells
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10.1007/s00044-013-0745-7 |
| RBL-1 | IC50 |
18 μM
Compound: 38
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Inhibition of cyclooxygenase in rat RBL1 cells
Inhibition of cyclooxygenase in rat RBL1 cells
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10.1007/s00044-013-0745-7 |
3,4-Dimethoxychalcone (30 μM, 8 h) induces autophagic flux in HepG2 cells and U2OS cells[1].
3,4-Dimethoxychalcone (30 μM, 6 h) stimulates the translocation of TFEB and TFE3 to the nucleus in U2OS cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 5416-71-7
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Appearance Solid
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Molecular Weight 268.31
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Formula C17H16O3
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Color Light yellow to green yellow
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SMILES
O=C(C1=CC=CC=C1)/C=C/C2=CC=C(OC)C(OC)=C2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
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Journal Impact Factor
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Most Recent
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FASEB J
3,4-Dimethoxychalcone Protects Against Steroid Induced Femoral Head Necrosis by Suppressing Ferroptosis via Activation of STAT3-Nrf2 Signaling Pathway. [Abstract]2026 Jun 30;40(12):e72021. PMID: 42268673
Solvent & Solubility
DMSO : 100 mg/mL (372.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.32 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Okolo EN, et al. New chalcone derivatives as potential antimicrobial and antioxidant agent. Sci Rep. 2021 Nov 5;11(1):21781. [Content Brief]
[2]. Chen G, et al. 3,4-Dimethoxychalcone induces autophagy through activation of the transcription factors TFE3 and TFEB. EMBO Mol Med. 2019 Nov 7;11(11):e10469. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.7270 mL | 18.6352 mL | 37.2703 mL | 93.1758 mL |
| 5 mM | 0.7454 mL | 3.7270 mL | 7.4541 mL | 18.6352 mL | |
| 10 mM | 0.3727 mL | 1.8635 mL | 3.7270 mL | 9.3176 mL | |
| 15 mM | 0.2485 mL | 1.2423 mL | 2.4847 mL | 6.2117 mL | |
| 20 mM | 0.1864 mL | 0.9318 mL | 1.8635 mL | 4.6588 mL | |
| 25 mM | 0.1491 mL | 0.7454 mL | 1.4908 mL | 3.7270 mL | |
| 30 mM | 0.1242 mL | 0.6212 mL | 1.2423 mL | 3.1059 mL | |
| 40 mM | 0.0932 mL | 0.4659 mL | 0.9318 mL | 2.3294 mL | |
| 50 mM | 0.0745 mL | 0.3727 mL | 0.7454 mL | 1.8635 mL | |
| 60 mM | 0.0621 mL | 0.3106 mL | 0.6212 mL | 1.5529 mL | |
| 80 mM | 0.0466 mL | 0.2329 mL | 0.4659 mL | 1.1647 mL | |
| 100 mM | 0.0373 mL | 0.1864 mL | 0.3727 mL | 0.9318 mL |
3,4-Dimethoxychalcone Related Classifications
HY-W083373A Related Classifications
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