2. Anti-infection Immunology/Inflammation
  3. Fungal Interleukin Related
  4. 3-Methylcarbazole

3-Methylcarbazole  (Synonyms: NSC 10154)

Cat. No.: HY-W047478 Purity: 97.78%
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3-Methylcarbazole (NSC 10154) is a carbazole alkaloid with an IC50 of 25 μg/mL against human fibrosarcoma cells. 3-Methylcarbazole inhibits mycelial growth and conidial germination of a variety of phytopathogenic fungi. 3-Methylcarbazole exhibits anti-inflammatory and antitumor activities. 3-Methylcarbazole can be used in studies related to fibrosarcoma, phytopathogenic fungal infections and inflammatory diseases.

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3-Methylcarbazole

3-Methylcarbazole Chemical Structure

CAS No. : 4630-20-0

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Other Forms of 3-Methylcarbazole:

3-Methylcarbazole Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

3-Methylcarbazole (NSC 10154) is a carbazole alkaloid with an IC50 of 25 μg/mL against human fibrosarcoma cells. 3-Methylcarbazole inhibits mycelial growth and conidial germination of a variety of phytopathogenic fungi. 3-Methylcarbazole exhibits anti-inflammatory and antitumor activities. 3-Methylcarbazole can be used in studies related to fibrosarcoma, phytopathogenic fungal infections and inflammatory diseases[1][2][3].

In Vitro

3-Methylcarbazole inhibits the growth of human fibrosarcoma HT-1080 cells, with an IC50 of 25 μg/mL[1].
3-Methylcarbazole (50-200 μg/mL; 17 h) exhibits no cell cycle inhibitory or apoptosis-inducing activity in mouse tsFT210 cells[1].
3-Methylcarbazole (0-480 µg/mL; 0-5 days) inhibits mycelial growth of Alternaria porri, Colletotrichum gloeosporioides, Colletotrichum musae, species of the genus Curvularia, species of the genus Drechslera, species of the genus Exserohilum, Fusarium oxysporum, species of the genus Verticillium, and Sclerotium rolfsii[2].
3-Methylcarbazole (30-480 µg/mL; 24 h) inhibits conidial germination of Colletotrichum gloeosporioides in a concentration-dependent manner[2].
3-Methylcarbazole (2.5-20 μg/mL; 30 min pretreatment) dose-dependently inhibits the production of NO, PGE2, TNF-α, IL-1β, IL-6 and IL-10 in LPS (HY-D1056)-stimulated RAW 264.7 murine macrophages[3].
3-Methylcarbazole (2.5-20 μg/mL; 30 min pretreatment) exhibits no cytotoxicity against RAW 264.7 murine macrophages even at concentrations as high as 20 μg/mL[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

3-Methylcarbazole Related Antibodies

Cell Cycle Analysis[1]

Cell Line: mouse tsFT210 cells
Concentration: 50 μg/mL, 200 μg/mL
Incubation Time: 17 h
Result: Showed no cell cycle inhibitory activity on tsFT210 cells at tested concentrations.
Showed no apoptosis inducing activity on tsFT210 cells at tested concentrations.
Detected no sub-G0/G1 peaks (apoptosis marker) via flow cytometry.
Observed no apoptotic morphological or nuclear changes.

Cell Viability Assay[3]

Cell Line: RAW 264.7 murine macrophages
Concentration: 2.5, 5, 10, 20 μg/mL
Incubation Time: 30 min pretreatment; 24 h incubation
Result: Showed no cytotoxic effects on RAW 264.7 cells at any tested concentration, with cell viability remaining at ~95-100% of control levels across all doses.
Molecular Weight

181.24

Formula

C13H11N
CAS No.
Appearance

Solid

Color

White to light brown

SMILES

CC1=CC2=C(C=C1)NC3=C2C=CC=C3
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (551.75 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.5175 mL 27.5877 mL 55.1755 mL
5 mM 1.1035 mL 5.5175 mL 11.0351 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.5 mg/mL (13.79 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 97.78%

SDS (393 KB)

COA (254 KB) HNMR (241 KB) LCMS (87 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 5.5175 mL 27.5877 mL 55.1755 mL 137.9386 mL
5 mM 1.1035 mL 5.5175 mL 11.0351 mL 27.5877 mL
10 mM 0.5518 mL 2.7588 mL 5.5175 mL 13.7939 mL
15 mM 0.3678 mL 1.8392 mL 3.6784 mL 9.1959 mL
20 mM 0.2759 mL 1.3794 mL 2.7588 mL 6.8969 mL
25 mM 0.2207 mL 1.1035 mL 2.2070 mL 5.5175 mL
30 mM 0.1839 mL 0.9196 mL 1.8392 mL 4.5980 mL
40 mM 0.1379 mL 0.6897 mL 1.3794 mL 3.4485 mL
50 mM 0.1104 mL 0.5518 mL 1.1035 mL 2.7588 mL
60 mM 0.0920 mL 0.4598 mL 0.9196 mL 2.2990 mL
80 mM 0.0690 mL 0.3448 mL 0.6897 mL 1.7242 mL
100 mM 0.0552 mL 0.2759 mL 0.5518 mL 1.3794 mL
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3-Methylcarbazole Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

3-Methylcarbazole4630-20-0NSC 10154NSC10154NSC-10154FungalInterleukin RelatedILTLR2human fibrosarcoma cellsColletotrichum musaeColletotrichum gloeosporioidesFusarium oxysporumSclerotium rolfsiiAlternaria porriClausena dunniana Levl.TLR4Streptomyces sp. LJK109Inhibitorinhibitorinhibit

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