4,5-Dicaffeoylquinic acid
Based on 12 publication(s) in Google Scholar
4,5-Dicaffeoylquinic acid (Isochlorogenic acid C) is an antioxidant, can be isolated from Gynura divaricata and Laggera alata. 4,5-Dicaffeoylquinic acid reduces islet cell apoptosis and improves pancreatic function in type 2 diabetic mice, and has obvious inhibitory activities against yeast α-glucosidase. 4,5-Dicaffeoylquinic acid inhibits prostate cancer cells through cell cycle arrest. 4,5-Dicaffeoylquinic acid also has anti-apoptotic, anti-injury and anti-hepatitis B virus effects.
For research use only. We do not sell to patients.
- Purity : 99.83%
- CAS No.: 57378-72-0
- Formula: C25H24O12
- Molecular Weight:516.45
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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HY-N0056
Isochlorogenic acid AAntioxidant -
HY-N0057
3,4-Dicaffeoylquinic acidAntioxidant -
HY-N0055
Chlorogenic acidAntioxidant -
HY-N0787
Cryptochlorogenic acidphenolic Acid Compound -
HY-N0722
Neochlorogenic acidCOX Inhibitor -
HY-N0603
20(S)-Ginsenoside Rg3Sodium Channel Inhibitor -
HY-15097
MyricetinAutophagy Inducer -
HY-N0359
CynarinAntioxidant -
HY-N0752
ScutellareinAutophagy Inducer -
HY-N0058R
4,5-Dicaffeoylquinic acid (Standard)Antioxidant -
HY-N0148
RutinCBR1 Inhibitor -
HY-N0060
Ferulic acidFGFR1 Inhibitor
Publications Citing Use of MedChemExpress (MCE) 4,5-Dicaffeoylquinic acid
More- Adv Sci (Weinh). 2025 Feb;12(6):e2410525. [Abstract]
- Phytomedicine. 2026 Feb:151:157776. [Abstract]
- Int J Biol Macromol. 2025 Mar:294:139536. [Abstract]
- J Agric Food Chem. 2021 Aug 18;69(32):9270-9286. [Abstract]
- J Agric Food Chem. 2019 Nov 6;67(44):12303-12312. [Abstract]
- Cell Biosci. 2023 Nov 14;13(1):210. [Abstract]
- Foods. 2024 Apr 3;13(7):1101. [Abstract]
- ACS Omega. 2025 Sep 22;10(38):44260-44269. [Abstract]
- Vet Microbiol. 2026 May:316:110992. [Abstract]
- Biologia. (2019) 74:1569-1577.
- SSRN. 2026 Apr 8.
- Chemistry of Plant Raw Material. 2025.
Customer Validation & Images
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WB
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WB
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IP
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Cell Imaging/Staining
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IHC
All Endogenous Metabolite Isoforms
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Biological Activity
Description
In Vitro
4,5-Dicaffeoylquinic acid (0.1~100 μM; 72 h) has dose-dependent inhibitory activity against DU-145 prostate cancer cells, and induces cell cycle arrest, also induces inactivation of Bcl-2[2].
4,5-Dicaffeoylquinic acid (1~100 μg/mL; 48 h) has anti-apoptotic effect in D-GalN-challenged HL-7702 hepatocytes, and improves significantly cell viability at concentrations of 10 to 100 μg/mL[3].
4,5-Dicaffeoylquinic acid (1~100 μg/mL; 4 days) inhibits significantly expressions of HBsAg and HBeAg, and produces the maximum inhibition rates of 86.93 and 59.79% at 100 μg/mL on expressions of HBsAg and HBeAg, respectively[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:DU-145 cells
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Concentration:0.1~100 µM
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Incubation Time:72 h
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Result:Exhibits dose-dependent inhibitory activity against DU-145 prostate cancer cells with an IC50 of 5 µM.
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Cell Line:DU-145 cells
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Concentration:5 µM
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Incubation Time:72 h
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Result:Decreased in numbers in G0/G1 phase and increased in S phase.
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Cell Line:HL-7702 hepatocytes (exposure to 60 mM D-GalN for 6h)
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Concentration:1~100 μg/mL
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Incubation Time:48 h
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Result:Significantly reduced the caspase-3 and transforming growth factor β1 (TGFβ1) levels of the D-GalN-challenged hepatocytes at 10~100 µg/mL.
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Cell Line:HL-7702 hepatocytes (exposure to 80 mM D-GalN for 6h)
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Concentration:1~100 μg/mL
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Incubation Time:48 h
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Result:Improved significantly cell viability at concentrations of 10 to 100 µg/mL, and produced a maximum protection rate of 47.28% at 100 µg/mL.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 57378-72-0
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Appearance Solid
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Molecular Weight 516.45
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Formula C25H24O12
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Color White to light yellow
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SMILES
O=C([C@]1(O)C[C@@H](OC(/C=C/C2=CC=C(O)C(O)=C2)=O)[C@@H](OC(/C=C/C3=CC=C(O)C(O)=C3)=O)[C@H](O)C1)O
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Synonyms
Isochlorogenic acid C; 4,5-diCQA
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Structure Classification
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (12)
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Journal Impact Factor
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Most Recent
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Adv Sci (Weinh)
Dipeptidyl Peptidase 4 (DPP4) Exacerbates Osteoarthritis Progression in an Enzyme-Independent Manner. [Abstract]2025 Feb;12(6):e2410525. PMID: 39680708
4,5-Dicaffeoylquinic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Feb;12(6):e2410525. [Abstract]
Different concentrations of 4,5-diCQA (4,5-Dicaffeoylquinic acid, 0-20 μM) were added to C28/I2 cells, and MYH9 levels were analyzed by western blot (n = 3 independent biological replicates).
4,5-Dicaffeoylquinic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Feb;12(6):e2410525. [Abstract]
Different concentrations of 4,5-diCQA (4,5-Dicaffeoylquinic acid, 0-20 μM) were added to DPP4-overexpressing C28/I2 cells, and MYH9 levels were analyzed by western blot (n = 3 independent biological replicates).
4,5-Dicaffeoylquinic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Feb;12(6):e2410525. [Abstract]
In C28/I2 cells transfected with MYH9-FLAG and DPP4‐HA plasmids, the addition of 4,5-diCQA (4,5-Dicaffeoylquinic acid), followed by IP pull-down of MYH9-FLAG and western blot analysis to detect DPP4‐HA levels (n = 3 independent biological replicates).
4,5-Dicaffeoylquinic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Feb;12(6):e2410525. [Abstract]
C28/I2 cells were treated with or without 4,5‐diCQA (4,5-Dicaffeoylquinic acid), and the interaction between DPP4 and MYH9 was analyzed using PLA.
4,5-Dicaffeoylquinic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Feb;12(6):e2410525. [Abstract]
IHC analysis of knee joints from Sham, DMM+PBS, and DMM+4,5-diCQA (4,5-Dicaffeoylquinic acid) groups.
4,5-Dicaffeoylquinic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Feb;12(6):e2410525. [Abstract]
Micro-CT analysis of knee joints from Sham, DMM+PBS, and DMM+4,5-diCQA (4,5-Dicaffeoylquinic acid) groups, including quantitative analysis of osteophyte number and SBP thickness.
4,5-Dicaffeoylquinic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Feb;12(6):e2410525. [Abstract]
Schematic diagram of the experimental design: 10-week-old mice underwent Sham or DMM surgery. One week after surgery, the DMM group received weekly intra-articular injections of PBS or 4,5-diCQA (4,5-Dicaffeoylquinic acid) until 8 weeks after surgery, when knee joints were collected. Schematic diagram of the experimental design: 18-month-old mice received intra-articular injections of AAV2-NC, AAV2-Dpp4-WT, or AAV2-Dpp4-MUT. Two weeks later, AAV2-NC mice received weekly PBS injections, while AAV2-Dpp4-WT and AAV2-Dpp4-MUT mice received weekly 4,5-diCQA injections. Knee joints were collected 3 months later.
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Phytomedicine
A novel mechanism of Reduning injection in sepsis treatment: Targeting inflammatory kinases TBK1 and IKKβ. [Abstract]2026 Feb:151:157776. PMID: 41512388 -
Int J Biol Macromol
2025 Mar:294:139536. PMID: 39765299 -
J Agric Food Chem
Raw and Sous-Vide-Cooked Red Cardoon Stalks ( Cynara cardunculus L. var. altilis DC): (Poly)phenol Bioaccessibility, Anti-inflammatory Activity in the Gastrointestinal Tract, and Prebiotic Activity. [Abstract]2021 Aug 18;69(32):9270-9286. PMID: 34347467 -
J Agric Food Chem
Isolation, Identification, and Synthesis of a New Prenylated Cinnamic Acid Derivative from Brazilian Green Propolis and Simultaneous Quantification of Bioactive Components by LC-MS/MS. [Abstract]2019 Nov 6;67(44):12303-12312. PMID: 31597041 -
Cell Biosci
2023 Nov 14;13(1):210. PMID: 37964389 -
Foods
Effects of Cooking Methods on Caffeoylquinic Acids and Radical Scavenging Activity of Sweet Potato. [Abstract]2024 Apr 3;13(7):1101. PMID: 38611405 -
ACS Omega
Selective Enhancement of Caffeoylquinic Acid Derivative via UV Irradiation and Validation of Analytical Method in the Aerial Aster × chusanensis Y. S. Lim. [Abstract]2025 Sep 22;10(38):44260-44269. PMID: 41048756 -
Vet Microbiol
The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]2026 May:316:110992. PMID: 41865607 -
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Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (193.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.84 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.84 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocols
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Apoptosis
Apoptosis, also called programmed cell death, is generally characterized by distinct morphological characteristics.
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TUNEL staining for apoptotic DNA fragmentation
TUNEL staining detects DNA strand breaks by using terminal deoxynucleotidyl transferase to add labeled nucleotides to exposed 3′-OH DNA termini, generating either microscopic staining in fixed cells or tissue sections, or fluorescence/cytometric signal in cell suspensions. TUNEL positivity reflects DNA fragmentation but should not be interpreted alone as definitive apoptosis, because TUNEL can also label necrotic, autolytic, mechanically damaged, or DNA-repair-associated DNA breaks.
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Flow cytometric DNA-content cell-cycle staining
Flow cytometric DNA-content cell-cycle staining measures the fluorescence intensity of DNA-bound fluorochromes in single cells or nuclei to estimate DNA content distributions, allowing assignment of populations to G0/G1, S, and G2/M phases by DNA histogram deconvolution. Propidium iodide (PI) intercalates into DNA, and PI fluorescence is proportional to cellular DNA content when staining is performed under conditions that make DNA accessible and minimize non-DNA signal. Cells with G2/M DNA content are expected to show approximately twice the fluorescence intensity of G0/G1 cells, while S-phase cells occupy intermediate fluorescence values. PI-based DNA-content analysis can also detect cells with fractional DNA content, often reported as sub-G1, when DNA fragmentation and extraction during staining reduce retained DNA signal in apoptotic cells. DAPI is an alternative DNA fluorochrome for univariate DNA-content analysis, while bivariate approaches combining DNA content with proliferation
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Annexin V plus membrane-impermeant dye apoptosis staining
Annexin V-based apoptosis assays rely on the detection of phosphatidylserine (PS) externalization from the inner leaflet of the plasma membrane to the outer leaflet, an early biochemical hallmark of apoptosis. Fluorescently labeled Annexin V binds PS in a calcium-dependent manner, enabling identification of early apoptotic cells by flow cytometry or fluorescence microscopy. When combined with a membrane-impermeant DNA-binding dye (e. g. , propidium iodide), this approach allows discrimination between viable (Annexin V−/dye−), early apoptotic (Annexin V+/dye−), and late apoptotic or necrotic (Annexin V+/dye+) cell populations by assessing membrane integrity and PS exposure.
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BrdU Incorporation Assay
Bromodeoxyuridine (BrdU) incorporation assay is based on the principle that BrdU, a thymidine analog, is incorporated into newly synthesized DNA during the S phase of the cell cycle, thereby serving as a marker of DNA replication and cellular proliferation. Incorporated BrdU can be detected using anti-BrdU antibodies following DNA denaturation, enabling visualization or quantification of proliferating cells through immunochemical detection methods such as immunofluorescence or immunohistochemistry.
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Apoptosis Solutions
Apoptosis is a regulated, generally non-lytic cell-death pathway that removes unwanted, damaged, infected, or abnormal cells through coordinated morphological changes, caspase activation, DNA fragmentation, and membrane remodeling. The intrinsic apoptosis pathway is controlled mainly by mitochondrial outer membrane permeabilization, BCL-2 family proteins, cytochrome c release, apoptosome formation, caspase-9 activation, and downstream executioner caspase-3/7 activation. The extrinsic apoptosis pathway is initiated by death receptors such as Fas, TNFR, and TRAIL receptors, which recruit adaptor proteins and activate caspase-8 before engaging executioner caspases or mitochondrial amplification through BID cleavage. Apoptosis is linked to many phenotypes, including cancer cell killing, tissue homeostasis, immune regulation, neurodegeneration, infection response, and treatment-induced cytotoxicity; unresolved questions include how apoptosis interacts with necroptosis, pyroptosis, ferroptos
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Protocol for Cell Cycle
Cell-cycle analysis by flow cytometry measures DNA content in single cells to estimate the fraction of cells in G0/G1, S, and G2/M phases. Propidium iodide intercalates into DNA, and after RNA removal with RNase, fluorescence intensity reflects cellular DNA content: 2N cells are assigned to G0/G1, cells between 2N and 4N to S phase, and 4N cells to G2/M. DNA-content analysis alone cannot reliably separate G0 from G1 or G2 from M. Ki-67 can distinguish quiescent G0 cells from cycling cells, EdU or BrdU incorporation marks active DNA synthesis in S phase, and phospho-histone H3 staining identifies mitotic cells within the 4N population.
Purity & Documentation
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Data Sheet (283 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Yin XL, et al. Gynura divaricata rich in 3, 5-/4, 5-dicaffeoylquinic acid and chlorogenic acid reduces islet cell apoptosis and improves pancreatic function in type 2 diabetic mice. Nutr Metab (Lond). 2018 Oct 10;15:73. [Content Brief]
[2]. Lodise O, et al. Inhibition of Prostate Cancer Cells by 4,5-Dicaffeoylquinic Acid through Cell Cycle Arrest. Prostate Cancer. 2019 May 23;2019:4520645. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.9363 mL | 9.6815 mL | 19.3630 mL | 48.4074 mL |
| 5 mM | 0.3873 mL | 1.9363 mL | 3.8726 mL | 9.6815 mL | |
| 10 mM | 0.1936 mL | 0.9681 mL | 1.9363 mL | 4.8407 mL | |
| 15 mM | 0.1291 mL | 0.6454 mL | 1.2909 mL | 3.2272 mL | |
| 20 mM | 0.0968 mL | 0.4841 mL | 0.9681 mL | 2.4204 mL | |
| 25 mM | 0.0775 mL | 0.3873 mL | 0.7745 mL | 1.9363 mL | |
| 30 mM | 0.0645 mL | 0.3227 mL | 0.6454 mL | 1.6136 mL | |
| 40 mM | 0.0484 mL | 0.2420 mL | 0.4841 mL | 1.2102 mL | |
| 50 mM | 0.0387 mL | 0.1936 mL | 0.3873 mL | 0.9681 mL | |
| 60 mM | 0.0323 mL | 0.1614 mL | 0.3227 mL | 0.8068 mL | |
| 80 mM | 0.0242 mL | 0.1210 mL | 0.2420 mL | 0.6051 mL | |
| 100 mM | 0.0194 mL | 0.0968 mL | 0.1936 mL | 0.4841 mL |
4,5-Dicaffeoylquinic acid Related Classifications
HY-N0058 Related Classifications
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