4-Hydroxydiphenylamine
Based on 1 Customer Validation
4-Hydroxydiphenylamine (p-Anilinophenol; 4-Hydroxy-DPA; 4-HDPA) is an aromatic amine organic compound. 4-Hydroxydiphenylamine exhibits antioxidant activity, and also acts as a catalyst or intermediate in certain chemical reactions to participate in the synthesis of other compounds. 4-Hydroxydiphenylamine can be used in research in the fields of environmental science and toxicology.
For research use only. We do not sell to patients.
- Purity: 98.02%
- CAS No.: 122-37-2
- Formula: C12H11NO
- Molecular Weight:185.22
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Storage:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
4-Hydroxydiphenylamine is a metabolite produced by activated sludge microorganisms[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 122-37-2
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Appearance Solid
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Molecular Weight 185.22
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Formula C12H11NO
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Color Brown to breen
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SMILES
OC1=CC=C(C=C1)NC2=CC=CC=C2
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Synonyms
p-Anilinophenol; 4-Hydroxy-DPA; 4-HDPA
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
DMSO : 100 mg/mL (539.90 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (26.99 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (26.99 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (555 KB)
- English - EN (555 KB)
- Français - FR (555 KB)
- Deutsch - DE (555 KB)
- Norwegian - NO (555 KB)
- Español - ES (555 KB)
- Swedish - SV (555 KB)
- Italian - IT (555 KB)
- Korean - KR (555 KB)
- Portuguese - PT (555 KB)
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Handling Instructions (2659 KB)
References
[1]. Gardner AM, et al. Microbial degradation of 14C-diphenylamine in a laboratory model sewage sludge system. Bull Environ Contam Toxicol. 1982 Jan;28(1):91-6. [Content Brief]
[4]. Alexander WE, et al. Metabolism of diphenylamine in the rat, rabbit and man. Food Cosmet Toxicol. 1965 Oct;3(4):571-9. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.3990 mL | 26.9949 mL | 53.9898 mL | 134.9746 mL |
| 5 mM | 1.0798 mL | 5.3990 mL | 10.7980 mL | 26.9949 mL | |
| 10 mM | 0.5399 mL | 2.6995 mL | 5.3990 mL | 13.4975 mL | |
| 15 mM | 0.3599 mL | 1.7997 mL | 3.5993 mL | 8.9983 mL | |
| 20 mM | 0.2699 mL | 1.3497 mL | 2.6995 mL | 6.7487 mL | |
| 25 mM | 0.2160 mL | 1.0798 mL | 2.1596 mL | 5.3990 mL | |
| 30 mM | 0.1800 mL | 0.8998 mL | 1.7997 mL | 4.4992 mL | |
| 40 mM | 0.1350 mL | 0.6749 mL | 1.3497 mL | 3.3744 mL | |
| 50 mM | 0.1080 mL | 0.5399 mL | 1.0798 mL | 2.6995 mL | |
| 60 mM | 0.0900 mL | 0.4499 mL | 0.8998 mL | 2.2496 mL | |
| 80 mM | 0.0675 mL | 0.3374 mL | 0.6749 mL | 1.6872 mL | |
| 100 mM | 0.0540 mL | 0.2699 mL | 0.5399 mL | 1.3497 mL |
4-Hydroxydiphenylamine Related Classifications
HY-W088320 Related Classifications
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