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  4. 5-Hydroxytryptophol-O-glucuronide

5-Hydroxytryptophol-O-glucuronide  (Synonyms: UGT1A6)

Cat. No.: HY-N13388 Purity: 99.86%
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5-Hydroxytryptophol-O-glucuronide is an authentic glucuronide standard and probe metabolite. 5-Hydroxytryptophol-O-glucuronide is used to detect the activity of human UGT1A6 in in vitro systems including human liver microsomes and recombinant UGT1A6.

For research use only. We do not sell to patients.

5-Hydroxytryptophol-O-glucuronide

5-Hydroxytryptophol-O-glucuronide Chemical Structure

CAS No. : 27939-38-4

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1 mg In-stock
5 mg In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of 5-Hydroxytryptophol-O-glucuronide:

5-Hydroxytryptophol-O-glucuronide Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

5-Hydroxytryptophol-O-glucuronide is an authentic glucuronide standard and probe metabolite. 5-Hydroxytryptophol-O-glucuronide is used to detect the activity of human UGT1A6 in in vitro systems including human liver microsomes and recombinant UGT1A6[1].

In Vitro

5-Hydroxytryptophol-O-glucuronide (0.025 mg/mL HLM protein; 20-10000 µM 5HTOL; 20 min) formation via UGT1A6 in pooled human liver microsomes exhibits weak substrate inhibition kinetics with a mean Km of 420 µM without 2% BSA and 330 µM with 2% BSA, and minimal changes in unbound intrinsic clearance with BSA addition[1].
5-Hydroxytryptophol-O-glucuronide (0.025 mg/mL rUGT1A6 protein; 20-10000 µM 5HTOL; 90 min) formation via recombinant UGT1A6 exhibits weak substrate inhibition kinetics with a mean Km of 570 µM without 2% BSA and 490 µM with 2% BSA, and a small increase in intrinsic clearance with BSA addition[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

5-Hydroxytryptophol-O-glucuronide Related Antibodies
Molecular Weight

353.32

Formula

C16H19NO8
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1[C@H](OC2=CC=C3NC=C(CCO)C3=C2)O[C@H](C(O)=O)[C@@H](O)[C@@H]1O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (283.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8303 mL 14.1515 mL 28.3030 mL
5 mM 0.5661 mL 2.8303 mL 5.6606 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (7.08 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (7.08 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.8303 mL 14.1515 mL 28.3030 mL 70.7574 mL
5 mM 0.5661 mL 2.8303 mL 5.6606 mL 14.1515 mL
10 mM 0.2830 mL 1.4151 mL 2.8303 mL 7.0757 mL
15 mM 0.1887 mL 0.9434 mL 1.8869 mL 4.7172 mL
20 mM 0.1415 mL 0.7076 mL 1.4151 mL 3.5379 mL
25 mM 0.1132 mL 0.5661 mL 1.1321 mL 2.8303 mL
30 mM 0.0943 mL 0.4717 mL 0.9434 mL 2.3586 mL
40 mM 0.0708 mL 0.3538 mL 0.7076 mL 1.7689 mL
50 mM 0.0566 mL 0.2830 mL 0.5661 mL 1.4151 mL
60 mM 0.0472 mL 0.2359 mL 0.4717 mL 1.1793 mL
80 mM 0.0354 mL 0.1769 mL 0.3538 mL 0.8845 mL
100 mM 0.0283 mL 0.1415 mL 0.2830 mL 0.7076 mL
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5-Hydroxytryptophol-O-glucuronide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

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