5α-Cholestane-d6
Based on 1 Customer Validation
5α-Cholestane-d6 is the deuterated-labeled 5α-Cholestane (HY-133970). 5α-Cholestane is a steroidal hydrocarbon and fecal stanol, and is used as an internal standard for gas chromatographic sterol analysis. 5α-Cholestane is the parent steroidal skeleton of polyhydroxylated brassinosteroids of plant origin. It is used as an internal standard for GC-FID and GC-MS analysis of fecal sterols in urine and sludge samples. 5α-Cholestane can be used in studies of age- or disease-related skeletal muscle atrophy.
For research use only. We do not sell to patients.
- Purity : 99.78%
- CAS No.: 358731-18-7
- Formula: C27H42D6
- Molecular Weight:378.71
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Application
1. This compound can be used as a tracer
2. This compound can be used as an internal standard for quantitative analysis by NMR, GC-MS, or LC-MS.
Chemical Information
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CAS No. 358731-18-7
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Unlabeled CAS 481-21-0
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Appearance Solid
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Molecular Weight 378.71
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Formula C27H42D6
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Color White to off-white
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SMILES
C[C@@]12[C@](CC[C@]2([H])[C@H](C)CCCC(C)C)([H])[C@@]3([H])[C@@](CC1)([H])[C@@]4([C@@](C([2H])([2H])C([2H])([2H])C([2H])([2H])C4)([H])CC3)C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
Ethanol : ≥ 20 mg/mL (52.81 mM)
DMF : ≥ 1 mg/mL (2.64 mM)
DMSO : ≥ 0.1 mg/mL (0.26 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (283 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
[1]. Esposito D, et al. Akt-dependent anabolic activity of natural and synthetic brassinosteroids in rat skeletal muscle cells. Journal of medicinal chemistry. 2011 Jun 23;54(12):4057-66. [Content Brief]
[2]. Schönning C, et al. Faecal contamination of source-separated human urine based on the content of faecal sterols. Water research. 2002 Apr;36(8):1965-72. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMF / Ethanol | 1 mM | 2.6405 mL | 13.2027 mL | 26.4054 mL | 66.0136 mL |
| Ethanol | 5 mM | 0.5281 mL | 2.6405 mL | 5.2811 mL | 13.2027 mL |
| 10 mM | 0.2641 mL | 1.3203 mL | 2.6405 mL | 6.6014 mL | |
| 15 mM | 0.1760 mL | 0.8802 mL | 1.7604 mL | 4.4009 mL | |
| 20 mM | 0.1320 mL | 0.6601 mL | 1.3203 mL | 3.3007 mL | |
| 25 mM | 0.1056 mL | 0.5281 mL | 1.0562 mL | 2.6405 mL | |
| 30 mM | 0.0880 mL | 0.4401 mL | 0.8802 mL | 2.2005 mL | |
| 40 mM | 0.0660 mL | 0.3301 mL | 0.6601 mL | 1.6503 mL | |
| 50 mM | 0.0528 mL | 0.2641 mL | 0.5281 mL | 1.3203 mL |
5α-Cholestane-d6 Related Classifications
HY-133970S Related Classifications
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Keywords
- 5α-Cholestane-d6
- 358731-18-7
- Isotope-Labeled Compounds
- Biochemical Assay Reagents
- Drug Intermediate
- gas chromatographic sterol analysis
- faecal sterols
- brassinosteroid analogues
- GC-FID
- skeletal muscle atrophy
- brassinosteroids
- faecal stanol
- GC-MS
- plant-derived polyhydroxylated brassinosteroids
- steroidal hydrocarbon
- Inhibitor
- inhibitor
- inhibit