5α-Cholestane
Based on 1 Customer Validation
5α-Cholestane is a steroidal hydrocarbon and fecal stanol, and is used as an internal standard for gas chromatographic sterol analysis. 5α-Cholestane is the parent steroidal skeleton of polyhydroxylated brassinosteroids of plant origin. It is used as an internal standard for GC-FID and GC-MS analysis of fecal sterols in urine and sludge samples. 5α-Cholestane can be used in studies of age- or disease-related skeletal muscle atrophy.
For research use only. We do not sell to patients.
- Purity : 99.34%
- CAS No.: 481-21-0
- Formula: C27H48
- Molecular Weight:372.67
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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HY-N1459
Campesterol -
HY-W020033
Lanosterol -
HY-N0131
Stigmasterol -
HY-133970R
5α-Cholestane (Standard) -
HY-B0185
Lidocaine -
HY-118103
Coprostanol -
HY-N0131R
Stigmasterol (Standard) -
HY-B0185R
Lidocaine (Standard) -
HY-W020033R
Lanosterol (Standard) -
HY-N4103A
Δ5-Avenasterol -
HY-118103R
Coprostanol (Standard) -
HY-B0185G
Lidocaine (GMP)
Biological Activity
Description
In Vitro
5α-Cholestane functions as an internal standard for gas chromatographic quantification of faecal sterols and stanols in source-separated human urine and sludge samples[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 481-21-0
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Appearance Solid
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Molecular Weight 372.67
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Formula C27H48
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Color White to off-white
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SMILES
CC(C)CCC[C@@H](C)[C@H]1CC[C@@]2([H])[C@]3([H])CC[C@@]4([H])CCCC[C@]4(C)[C@@]3([H])CC[C@]12C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
Ethanol : 4 mg/mL (10.73 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (585 KB)
- English - EN (585 KB)
- Français - FR (585 KB)
- Deutsch - DE (585 KB)
- Norwegian - NO (585 KB)
- Español - ES (585 KB)
- Swedish - SV (585 KB)
- Italian - IT (585 KB)
- Korean - KR (585 KB)
- Portuguese - PT (585 KB)
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Handling Instructions (2659 KB)
References
[1]. Esposito D, et al. Akt-dependent anabolic activity of natural and synthetic brassinosteroids in rat skeletal muscle cells. Journal of medicinal chemistry. 2011 Jun 23;54(12):4057-66. [Content Brief]
[2]. Schönning C, et al. Faecal contamination of source-separated human urine based on the content of faecal sterols. Water research. 2002 Apr;36(8):1965-72. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Ethanol | 1 mM | 2.6833 mL | 13.4167 mL | 26.8334 mL | 67.0835 mL |
| 5 mM | 0.5367 mL | 2.6833 mL | 5.3667 mL | 13.4167 mL | |
| 10 mM | 0.2683 mL | 1.3417 mL | 2.6833 mL | 6.7083 mL |
5α-Cholestane Related Classifications
HY-133970 Related Classifications
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