1. Metabolic Enzyme/Protease
  2. Drug Metabolite
  3. 7-Hydroxymethotrexate

7-Hydroxymethotrexate 

Cat. No.: HY-130569
Handling Instructions

7-Hydroxymethotrexate is a major metabolite of Methotrexate (MTX; HY-14519). Methotrexate, an antimetabolite and antifolate agent, inhibits the enzyme dihydrofolate reductase, thereby preventing the conversion of folic acid into tetrahydrofolate, and inhibiting DNA synthesis.

For research use only. We do not sell to patients.

7-Hydroxymethotrexate Chemical Structure

7-Hydroxymethotrexate Chemical Structure

CAS No. : 5939-37-7

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Description

7-Hydroxymethotrexate is a major metabolite of Methotrexate (MTX; HY-14519). Methotrexate, an antimetabolite and antifolate agent, inhibits the enzyme dihydrofolate reductase, thereby preventing the conversion of folic acid into tetrahydrofolate, and inhibiting DNA synthesis[1][2].

In Vitro

7-Hydroxymethotrexate (7-OHMTX) has the affinity of DHFR is markedly lower (>100-fold) than for Methotrexate (MTX) [1].

In Vivo

7-Hydroxymethotrexate (4 mg/kg; IV) has a terminal half-life of 97.2 min and a total clearance value of 9.6 mL/min•mg[1].

Animal Model: Male Wistar rats (270-440 g)[1]
Dosage: 4 mg/kg
Administration: IV
Result: Had a terminal half-life of 97.2 min and a total clearance value of 9.6 mL/min•mg.
Molecular Weight

470.44

Formula

C₂₀H₂₂N₈O₆

CAS No.

5939-37-7

SMILES

O=C(O)CC[[email protected]@H](C(O)=O)NC(C1=CC=C(N(CC2=NC3=C(N)N=C(N)N=C3NC2=O)C)C=C1)=O

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Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

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Keywords:

7-HydroxymethotrexateDrug MetaboliteMethotrexateMTXantimetaboliteantifolateenzymedihydrofolatereductasetetrahydrofolateDNAsynthesisInhibitorinhibitorinhibit

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7-Hydroxymethotrexate
Cat. No.:
HY-130569
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