2. Metabolic Enzyme/Protease
  3. Drug Metabolite
  4. 7-Hydroxymethotrexate

7-Hydroxymethotrexate is a major metabolite of Methotrexate (MTX; HY-14519). Methotrexate, an antimetabolite and antifolate agent, inhibits the enzyme dihydrofolate reductase, thereby preventing the conversion of folic acid into tetrahydrofolate, and inhibiting DNA synthesis.

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7-Hydroxymethotrexate Chemical Structure

7-Hydroxymethotrexate Chemical Structure

CAS No. : 5939-37-7

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Other Forms of 7-Hydroxymethotrexate:

7-Hydroxymethotrexate Related Antibodies

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Description

7-Hydroxymethotrexate is a major metabolite of Methotrexate (MTX; HY-14519). Methotrexate, an antimetabolite and antifolate agent, inhibits the enzyme dihydrofolate reductase, thereby preventing the conversion of folic acid into tetrahydrofolate, and inhibiting DNA synthesis[1][2].

In Vitro

7-Hydroxymethotrexate (7-OHMTX) has the affinity of DHFR markedly lower (>100-fold) than for Methotrexate (MTX) [1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

7-Hydroxymethotrexate Related Antibodies
In Vivo

7-Hydroxymethotrexate (4 mg/kg; IV) has a terminal half-life of 97.2 min and a total clearance value of 9.6 mL/min•mg[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Wistar rats (270-440 g)[1]
Dosage: 4 mg/kg (Pharmacokinetic Analysis)
Administration: IV
Result: Had a terminal half-life of 97.2 min and a total clearance value of 9.6 mL/min•mg.
Molecular Weight

470.44

Formula

C20H22N8O6
CAS No.
SMILES

O=C(O)CC[C@@H](C(O)=O)NC(C1=CC=C(N(CC2=NC3=C(N)N=C(N)N=C3NC2=O)C)C=C1)=O
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Room temperature in continental US; may vary elsewhere.
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Please store the product under the recommended conditions in the Certificate of Analysis.
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References
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7-Hydroxymethotrexate Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

7-Hydroxymethotrexate5939-37-7Drug MetaboliteMethotrexateMTXantimetaboliteantifolateenzymedihydrofolatereductasetetrahydrofolateDNAsynthesisInhibitorinhibitorinhibit

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