Ac-CoA Synthase-IN-1
Based on 8 publication(s) in Google Scholar
Ac-CoA Synthase-IN-1 is a potent, reversible acetate-dependent acetyl-CoA synthetase 2 (ACSS2) inhibitor with an IC50 of 0.6 μM. Ac-CoA Synthase-IN-1 inhibits the respiratory syncytial virus (RSV).
For research use only. We do not sell to patients.
- Purity: 99.70%
- CAS No.: 508186-14-9
- Formula: C20H18N4O2S2
- Molecular Weight:410.51
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Ac-CoA Synthase-IN-1
More- Cell Metab. 2025 Sep 23:S1550-4131(25)00383-3. [Abstract]
- Sci Adv. 2023 Apr 14;9(15):eadf8522. [Abstract]
- Cell Death Dis. 2023 Nov 7;14(11):722. [Abstract]
- Liver Int. 2023 Aug;43(8):1729-1740. [Abstract]
- Sci Rep. 2024 Nov 15;14(1):28193. [Abstract]
- Toxicol Appl Pharmacol. 2022 Dec 15:457:116319. [Abstract]
- Biol Reprod. 2025 Oct 1:ioaf220. [Abstract]
- Res Sq. 2026 Jun 15.
Customer Validation & Images
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Others
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WB
Biological Activity
Ac-CoA Synthase-IN-1 inhibits cellular [14C]acetate uptake into both lipids and histones with an IC50 of 5 μM. Ac-CoA Synthase-IN-1 inhibits the ability of HepG2 cells to incorporate [14C]acetate into lipids with IC50 of 6.8 μM. Ac-CoA Synthase-IN-1 inhibits HepG2 utilization of [14C]acetate for histone acetylation with IC50 of 5.5 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 508186-14-9
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Appearance Solid
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Molecular Weight 410.51
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Formula C20H18N4O2S2
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Color Light yellow to yellow
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SMILES
O=C(NCCOC)NC1=CC=C2N=C(C3=CC=CS3)C(C4=CC=CS4)=NC2=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (8)
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Journal Impact Factor
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Most Recent
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Cell Metab
Hepatic acetyl-CoA metabolism modulates neuroinflammation and depression susceptibility via acetate. [Abstract]2025 Sep 23:S1550-4131(25)00383-3. PMID: 40992374 -
Sci Adv
M2 macrophages drive leukemic transformation by imposing resistance to phagocytosis and improving mitochondrial metabolism. [Abstract]2023 Apr 14;9(15):eadf8522. PMID: 37058562 -
Cell Death Dis
Pyruvate dehydrogenase kinase 1 protects against neuronal injury and memory loss in mouse models of diabetes. [Abstract]2023 Nov 7;14(11):722. PMID: 37935660
Ac-CoA Synthase-IN-1 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2023 Nov 7;14(11):722. [Abstract]
Western blot analysis of Ac-CoA Synthase-IN-1 (ACSS2-IN: 5 μM) and PDK1 in Neuro-2a cells after high glucose stimulation.
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Liver Int
Inhibition of ACSS2 attenuates alcoholic liver steatosis via epigenetically regulating de novo lipogenesis. [Abstract]2023 Aug;43(8):1729-1740. PMID: 37183518 -
Sci Rep
Contribution of glucose and glutamine to hypoxia-induced lipid synthesis decreases, while contribution of acetate increases, during 3T3-L1 differentiation. [Abstract]2024 Nov 15;14(1):28193. PMID: 39548264
Ac-CoA Synthase-IN-1 purchased from MedChemExpress. Usage Cited in: Sci Rep. 2024 Nov 15;14(1):28193. [Abstract]
Ac-CoA Synthase-IN-1 (20 µM). Effect of hypoxia on total lipid content after inhibition of the citrate or acetic acid-dependent pathway. Intracellular lipid accumulation in 3T3-L1 adipocytes after exposure to hypoxia for 7 or 14 days.
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Toxicol Appl Pharmacol
Cysteine and glycine rich protein 2 exacerbates vascular fibrosis in pulmonary hypertension through the nuclear translocation of yes-associated protein and transcriptional coactivator with PDZ-binding motif. [Abstract]2022 Dec 15:457:116319. PMID: 36414118 -
Biol Reprod
TGF-β3 Promotes Trophoblast Development in Sheep Embryos via ACSS2-Dependent Permissive Lipid Metabolism. [Abstract]2025 Oct 1:ioaf220. PMID: 41031631 -
Solvent & Solubility
DMSO : 31.25 mg/mL (76.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.07 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.07 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.4360 mL | 12.1800 mL | 24.3599 mL | 60.8999 mL |
| 5 mM | 0.4872 mL | 2.4360 mL | 4.8720 mL | 12.1800 mL | |
| 10 mM | 0.2436 mL | 1.2180 mL | 2.4360 mL | 6.0900 mL | |
| 15 mM | 0.1624 mL | 0.8120 mL | 1.6240 mL | 4.0600 mL | |
| 20 mM | 0.1218 mL | 0.6090 mL | 1.2180 mL | 3.0450 mL | |
| 25 mM | 0.0974 mL | 0.4872 mL | 0.9744 mL | 2.4360 mL | |
| 30 mM | 0.0812 mL | 0.4060 mL | 0.8120 mL | 2.0300 mL | |
| 40 mM | 0.0609 mL | 0.3045 mL | 0.6090 mL | 1.5225 mL | |
| 50 mM | 0.0487 mL | 0.2436 mL | 0.4872 mL | 1.2180 mL | |
| 60 mM | 0.0406 mL | 0.2030 mL | 0.4060 mL | 1.0150 mL |
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