1. Apoptosis
  2. Apoptosis
  3. MDK83190

MDK83190 

Cat. No.: HY-18633 Purity: 98.08%
Handling Instructions

MDK83190 is a potent apoptosis activator, induces Apaf-1 oligomerization, increases procaspase-9 processing and subsequent caspase-3 activation in a cyto c-dependent Manner.

For research use only. We do not sell to patients.

MDK83190 Chemical Structure

MDK83190 Chemical Structure

CAS No. : 79183-19-0

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply Now  
Solution
10 mM * 1 mL in DMSO USD 73 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 73 In-stock
Estimated Time of Arrival: December 31
Solid
10 mg USD 66 In-stock
Estimated Time of Arrival: December 31
50 mg USD 238 In-stock
Estimated Time of Arrival: December 31
100 mg USD 376 In-stock
Estimated Time of Arrival: December 31
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Description

MDK83190 is a potent apoptosis activator, induces Apaf-1 oligomerization, increases procaspase-9 processing and subsequent caspase-3 activation in a cyto c-dependent Manner[1].

IC50 & Target

IC50: 4-9 uM (Leukemia origin cells)[1].

In Vitro

MDK83190 (Compound 2) (1-50 μM; 2 hours) effects against cell lines of lymphoid origin (CCRF-CEM, MOLT-4, and Jurkat) with IC50 values ranging from 4 to 9 μM[1].
MDK83190 (Compound 2) (20 μM; 30 mins) activates procaspase-9 and procaspase-3 in a cyto c-dependent manner[1].
MDK83190 (Compound 2) (0.1-100 μM; 22 hours) strongly induces caspase-3 activation, PARP cleavage, and DNA fragmentation, and finally killing cells with an IC50 of 4 μM[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: Leukemia cell line: CCRF-CEM cells, MOLT-4 cells, and Jurkat cells
Concentration: 1-50 μM
Incubation Time: 2 hours
Result: Was sensitive to compound 2-induced apoptosis.

Western Blot Analysis[1]

Cell Line: Hela cells
Concentration: 20 μM
Incubation Time: 30 mins
Result: Induced procaspase-9 and procaspase-3 to active forms.

Apoptosis Analysis[1]

Cell Line: Jurkat cells
Concentration: 0.1-100 μM
Incubation Time: 22 hours
Result: Resulted in apoptosis and finally killed cells.
Molecular Weight

306.14

Formula

C15H9Cl2NO2

CAS No.
SMILES

O=C1N(CC2=CC=C(Cl)C(Cl)=C2)C3=C(C=CC=C3)C1=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 46 mg/mL (150.26 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2665 mL 16.3324 mL 32.6648 mL
5 mM 0.6533 mL 3.2665 mL 6.5330 mL
10 mM 0.3266 mL 1.6332 mL 3.2665 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (8.17 mM); Clear solution

*All of the co-solvents are available by MCE.
References
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Product Name:
MDK83190
Cat. No.:
HY-18633
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