Bilirubin
Based on 6 publication(s) in Google Scholar
Bilirubin is a yellow breakdown product of heme catabolism. Bilirubin exhibits antioxidant and antimutagenic effects.
For research use only. We do not sell to patients.
- Purity: 100.19%
- CAS No.: 635-65-4
- Formula: C33H36N4O6
- Molecular Weight:584.66
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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HY-L012
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HY-L021
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Human Endogenous Metabolite Compound Library
HY-L030
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HY-L037
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Publications Citing Use of MedChemExpress (MCE) Bilirubin
More- Nat Immunol. 2026 May;27(5):949-960. [Abstract]
- Hepatology. 2024 Aug 1;80(2):403-417. [Abstract]
- J Virol. 2026 Feb 17;100(2):e0165625. [Abstract]
- J Bone Oncol. 2023 Feb 23:39:100472. [Abstract]
- PLoS Negl Trop Dis. 2025 Dec 11;19(12):e0013815. [Abstract]
- Adv Med Educ Pract. 2021 Mar 4:12:237-243. [Abstract]
Customer Validation & Images
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RT-PCR
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Bio/Physico-chemical Assay
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WB
All Endogenous Metabolite Isoforms
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Biological Activity
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Human Endogenous Metabolite |
Unconjugated Bilirubin inhibits the cleavage of F485-rVWF73-H, D633-rVWF73-H, and GST-rVWF71-11K by ADAMTS13 in a concentration-dependent manner with a half-maximal inhibitory concentration (IC50) of ~13 μM, ~70 μM, and ~17 μM, respectively. Unconjugated Bilirubin also dose-dependently inhibits the cleavage of multimeric VWF by ADAMTS13 under denaturing conditions[1]. Bilirubin exhibits antioxidant and antimutagenic effects in vitro[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 635-65-4
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Appearance Solid
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Molecular Weight 584.66
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Formula C33H36N4O6
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Color Yellow to orange
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SMILES
CC(C(N/1)=O)=C(C=C)C1=C\C2=C(C)C(CCC(O)=O)=C(CC3=C(CCC(O)=O)C(C)=C(/C=C4NC(C(C=C)=C\4C)=O)N3)N2
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (6)
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Journal Impact Factor
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Most Recent
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Nat Immunol
Lipid asymmetry disruption by XKR8 orchestrates neutrophil extracellular trap formation and inhibits fungal infection. [Abstract]2026 May;27(5):949-960. PMID: 41781710 -
Hepatology
Serum aryl hydrocarbon receptor activity is associated with survival in patients with alcohol-associated hepatitis. [Abstract]2024 Aug 1;80(2):403-417. PMID: 38377466
Bilirubin purchased from MedChemExpress. Usage Cited in: Hepatology. 2024 Aug 1;80(2):403-417. [Abstract]
Verification of the function of bilirubin as an AhR ligand using HepG2-Lucia AhR reporter cells.
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J Virol
Seneca Valley virus 3C protease targets the Nrf2/HO-1 pathway to antagonize its antiviral activity. [Abstract]2026 Feb 17;100(2):e0165625. PMID: 41489372 -
J Bone Oncol
Indirect bilirubin impairs invasion of osteosarcoma cells via inhibiting the PI3K/AKT/MMP-2 signaling pathway by suppressing intracellular ROS. [Abstract]2023 Feb 23:39:100472. PMID: 36876225
Bilirubin purchased from MedChemExpress. Usage Cited in: J Bone Oncol. 2023 Feb 23:39:100472. [Abstract]
Bilirubin (IBIL; 15 μM) markedly decreases the expression of MMP-2 and upregulates the expression of TIMP-2 in 143B and MG63 cells.
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PLoS Negl Trop Dis
The phytochemical arbutin exerts potent anti-Toxoplasma effects through activation of cell-autonomous defense mechanisms while attenuating inflammation. [Abstract]2025 Dec 11;19(12):e0013815. PMID: 41379884
Bilirubin purchased from MedChemExpress. Usage Cited in: PLoS Negl Trop Dis. 2025 Dec 11;19(12):e0013815. [Abstract]
HFF cells were incubated with vehicle and bilirubin (10 μM or 40 μM) 2 h before infection with Tg RH (MOI = 0.2). The relative intracellular parasite numbers were quantified by qPCR at 72 h post-infection.
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Adv Med Educ Pract
Online Medical Education in India - Different Challenges and Probable Solutions in the Age of COVID-19. [Abstract]2021 Mar 4:12:237-243. PMID: 33692645
Solvent & Solubility
0.1 M NaOH : 9.09 mg/mL (15.55 mM; ultrasonic and warming and adjust pH to 11 with 0.1 N NaOH and heat to 60°C)
DMSO : 5.38 mg/mL (9.20 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
0.1 M HCl : < 1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 10 mg/mL (17.10 mM); Suspended solution; Need ultrasonic
Purity & Documentation
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Data Sheet (280 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Lu RN, et al. Unconjugated Bilirubin inhibits proteolytic cleavage of von Willebrand factor by ADAMTS13 protease. J Thromb Haemost. 2015 Jun;13(6):1064-72. [Content Brief]
[2]. Mölzer C, et al. Bilirubin and related tetrapyrroles inhibit food-borne mutagenesis: a mechanism for antigenotoxic action against a model epoxide. J Nat Prod. 2013 Oct 25;76(10):1958-65. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / 0.1 M NaOH | 1 mM | 1.7104 mL | 8.5520 mL | 17.1040 mL | 42.7599 mL |
| 5 mM | 0.3421 mL | 1.7104 mL | 3.4208 mL | 8.5520 mL | |
| 0.1 M NaOH | 10 mM | 0.1710 mL | 0.8552 mL | 1.7104 mL | 4.2760 mL |
| 15 mM | 0.1140 mL | 0.5701 mL | 1.1403 mL | 2.8507 mL |
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