CK-869
Based on 3 publication(s) in Google Scholar
CK-869 is an Actin-Related Protein 2/3 (ARP2/3) complex inhibitor, with an IC50 of 7 μM.
For research use only. We do not sell to patients.
- Purity : 99.84%
- CAS No.: 388592-44-7
- Formula: C17H16BrNO3S
- Molecular Weight:394.28
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) CK-869
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Customer Validation & Images
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Cell Imaging/Staining
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Cell Imaging/Staining
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Cell Imaging/Staining
Biological Activity
Description
IC50 & Target
IC50: 7 μM (ARP2/3)[1].
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| A549 | IC50 |
14.76 μM
Compound: 2, CK-869
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Antiproliferative activity against human A549 cells after 48 hrs by sulforhodamine B assay
Antiproliferative activity against human A549 cells after 48 hrs by sulforhodamine B assay
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[PMID: 24794770] |
| MDA-MB-231 | IC50 |
17.11 μM
Compound: 2, CK-869
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Antiproliferative activity against human MDA-MB-231 cells after 48 hrs by sulforhodamine B assay
Antiproliferative activity against human MDA-MB-231 cells after 48 hrs by sulforhodamine B assay
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[PMID: 24794770] |
In Vitro
CK-869 is an Actin-Related Protein 2/3 (ARP2/3) complex inhibitor, with an IC50 of 7 μM[1]. CK-869 significantly inhibits MT polymerization even at a concentration of 25 μM[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 388592-44-7
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Appearance Solid
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Molecular Weight 394.28
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Formula C17H16BrNO3S
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Color White to off-white
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SMILES
O=C1N(C2=CC=C(OC)C=C2OC)C(C3=CC=CC(Br)=C3)SC1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (3)
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Journal Impact Factor
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Most Recent
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Cell Rep
2021 Jul 6;36(1):109318. PMID: 34233185
CK-869 purchased from MedChemExpress. Usage Cited in: Cell Rep. 2021 Jul 6;36(1):109318. [Abstract]
Representative images and graphs representing the fold change of IS descriptors for NK-92 cells seeded on ICAM-1, anti-NKp30, and antiNKp46, stained for F-actin (green), perforin granules (yellow), and nuclei (blue) and treated with three concentrations of latrunculin B, jasplakinolide, blebbistatin, Y-27632, CK-869, wiskostatin, and SMIFH2 with respect to the untreated control.
CK-869 purchased from MedChemExpress. Usage Cited in: Cell Rep. 2021 Jul 6;36(1):109318. [Abstract]
Representative images of primary NK cells isolated from peripheral blood mononuclear cells (PBMCs) of three normal donors seeded on ICAM-1, anti-NKp30, and anti-NKp46, stained for F-actin (green), perforin granules (yellow), and nuclei (blue) and left untreated or treated with four concentrations of CK-869 (0-50 μM) or SMIFH2.
CK-869 purchased from MedChemExpress. Usage Cited in: Cell Rep. 2021 Jul 6;36(1):109318. [Abstract]
Graphs representing the fold changes of IS parameters of primary NK cells treated with CK-869 (0-50 μM) with respect to untreated controls.
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Transbound Emerg Dis
2025 Nov 21:2025:5822897. PMID: 41323147 -
Solvent & Solubility
In Vitro:
DMSO : 25 mg/mL (63.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.34 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.34 mM); Suspended solution
This protocol yields a suspended solution of ≥ 2.5 mg/mL (saturation unknown). Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
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Data Sheet (282 KB)
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SDS (761 KB)
- English - EN (761 KB)
- Français - FR (761 KB)
- Deutsch - DE (761 KB)
- Norwegian - NO (761 KB)
- Español - ES (761 KB)
- Swedish - SV (761 KB)
- Italian - IT (761 KB)
- Korean - KR (761 KB)
- Portuguese - PT (761 KB)
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Handling Instructions (2659 KB)
References
[1]. Hetrick B, et al. Small molecules CK-666 and CK-869 inhibit actin-related protein 2/3 complex by blocking an activating conformational change. Chem Biol. 2013 May 23;20(5):701-12. [Content Brief]
[2]. Yamagishi Y, et al. Use of CK-548 and CK-869 as Arp2/3 complex inhibitors directly suppresses microtubule assembly both in vitro and in vivo. Biochem Biophys Res Commun. 2018 Feb 12;496(3):834-839. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.5363 mL | 12.6813 mL | 25.3627 mL | 63.4067 mL |
| 5 mM | 0.5073 mL | 2.5363 mL | 5.0725 mL | 12.6813 mL | |
| 10 mM | 0.2536 mL | 1.2681 mL | 2.5363 mL | 6.3407 mL | |
| 15 mM | 0.1691 mL | 0.8454 mL | 1.6908 mL | 4.2271 mL | |
| 20 mM | 0.1268 mL | 0.6341 mL | 1.2681 mL | 3.1703 mL | |
| 25 mM | 0.1015 mL | 0.5073 mL | 1.0145 mL | 2.5363 mL | |
| 30 mM | 0.0845 mL | 0.4227 mL | 0.8454 mL | 2.1136 mL | |
| 40 mM | 0.0634 mL | 0.3170 mL | 0.6341 mL | 1.5852 mL | |
| 50 mM | 0.0507 mL | 0.2536 mL | 0.5073 mL | 1.2681 mL | |
| 60 mM | 0.0423 mL | 0.2114 mL | 0.4227 mL | 1.0568 mL |
CK-869 Related Classifications
HY-16927 Related Classifications
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