2. Anti-infection
  3. Bacterial
  4. Flagelin 22

Flagelin 22 (Flagellin 22), a fragment of bacterial flagellin, is an effective elicitor in both plants and algae.

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CAS No. : 304642-91-9

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Based on 5 publication(s) in Google Scholar

Other Forms of Flagelin 22:

Flagelin 22 Related Antibodies

Top Publications Citing Use of Products

    Flagelin 22 purchased from MedChemExpress. Usage Cited in: Plant Biotechnol J. 2021 Nov;19(11):2319-2332.  [Abstract]

    The relative expression levels of the PTI‐ and MAPK‐related genes in response to flg22 in NIP plants at assessed by RT‐qPCR. NIP plants were infiltrated with 10 μM Flagelin 22 (flg22), and leaves were collected 30 min post‐infiltration for transcript analysis.

    Flagelin 22 purchased from MedChemExpress. Usage Cited in: Plant Biotechnol J. 2021 Nov;19(11):2319-2332.  [Abstract]

    MAPK activation in NIP and osrlp1‐cas‐1# rice leaves after exogenous 10 μM Flagelin 22 (flg22) treatment for 0, 5, 10, 20 or 30 min. Anti‐p42/44 antibody was used for Western blotting.

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Flagelin 22 (Flagellin 22), a fragment of bacterial flagellin, is an effective elicitor in both plants and algae[1][2][3].

    In Vitro

    Flagelin 22 (100 nM, 1 or 24 h) activates a gene that triggers immunity (PTI) and leads to reduced expression of growth-promoting hormone brassinolide (BR) biosynthesis in plants[2].
    Flagelin 22 (10 μM, 4 h) regulates the release of ethylene reactive factor substrate in Arabidopsis MAP kinase 6 by ethylene signaling[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Flagelin 22 Related Antibodies
    Molecular Weight

    2272.48

    Formula

    C93H162N32O34
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    Sequence

    Gln-Arg-Leu-Ser-Thr-Gly-Ser-Arg-Ile-Asn-Ser-Ala-Lys-Asp-Asp-Ala-Ala-Gly-Leu-Gln-Ile-Ala
    Sequence Shortening

    QRLSTGSRINSAKDDAAGLQIA
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Sealed storage, away from moisture

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : 100 mg/mL (44.00 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.4400 mL 2.2002 mL 4.4005 mL
    5 mM 0.0880 mL 0.4400 mL 0.8801 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 97.61%

    SDS (252 KB)

    COA (243 KB) RP-HPLC (267 KB) LCMS (90 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [1]

    One milliliter of the female gametophytes is added to 100 mL sterilized seawater containing 1 μM of Flagelin 22, flg15, flg14, and flg22D43A, respectively. Controls are sterilized seawater and 0.1% BSA in sterilized seawater. Gametophytes are grown at 10°C with a 24-h photoperiod at 50 μmol photons m-2 s-1. Sterilized seawater medium is provided with 0.2 mM KNO3, 0.02mM KH2PO4, and 1 μM of the four respective peptides and refreshed every week. The gametophytes are briefly blotted dry, and the fresh weight is measured after 40 days[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 0.4400 mL 2.2002 mL 4.4005 mL 11.0012 mL
    5 mM 0.0880 mL 0.4400 mL 0.8801 mL 2.2002 mL
    10 mM 0.0440 mL 0.2200 mL 0.4400 mL 1.1001 mL
    15 mM 0.0293 mL 0.1467 mL 0.2934 mL 0.7334 mL
    20 mM 0.0220 mL 0.1100 mL 0.2200 mL 0.5501 mL
    25 mM 0.0176 mL 0.0880 mL 0.1760 mL 0.4400 mL
    30 mM 0.0147 mL 0.0733 mL 0.1467 mL 0.3667 mL
    40 mM 0.0110 mL 0.0550 mL 0.1100 mL 0.2750 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Flagelin 22 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Flagelin 22304642-91-9Flagellin 22Flagelin22Flagelin-22Flagellin22Flagellin-22BacterialFlg22PTIBRMAPK6Plant InducersInhibitorinhibitorinhibit

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    Product Name:
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    Cat. No.:
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