Flagelin 22
Based on 6 publication(s) in Google Scholar
Flagelin 22 (Flagellin 22), a fragment of bacterial flagellin, is an effective elicitor in both plants and algae.
For research use only. We do not sell to patients.
- Purity: 97.61%
- CAS No.: 304642-91-9
- Formula: C93H162N32O34
- Molecular Weight:2272.48
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Flagelin 22
More
Customer Validation & Images
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RT-PCR
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WB
Biological Activity
Flagelin 22 (100 nM, 1 or 24 h) activates a gene that triggers immunity (PTI) and leads to reduced expression of growth-promoting hormone brassinolide (BR) biosynthesis in plants[2].
Flagelin 22 (10 μM, 4 h) regulates the release of ethylene reactive factor substrate in Arabidopsis MAP kinase 6 by ethylene signaling[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 304642-91-9
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Appearance Solid
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Molecular Weight 2272.48
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Formula C93H162N32O34
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Color White to off-white
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Synonyms
Flagellin 22
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Sequence
Gln-Arg-Leu-Ser-Thr-Gly-Ser-Arg-Ile-Asn-Ser-Ala-Lys-Asp-Asp-Ala-Ala-Gly-Leu-Gln-Ile-Ala
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Sequence Shortening
QRLSTGSRINSAKDDAAGLQIA
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (6)
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Journal Impact Factor
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Most Recent
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Cell
2025 Mar 6;188(5):1330-1348.e27. PMID: 39855200 -
Nat Commun
Lanthanum(III) triggers AtrbohD- and jasmonic acid-dependent systemic endocytosis in plants. [Abstract]2021 Jul 15;12(1):4327. PMID: 34267202 -
Plant Biotechnol J
A rice LRR receptor-like protein associates with its adaptor kinase OsSOBIR1 to mediate plant immunity against viral infection. [Abstract]2021 Nov;19(11):2319-2332. PMID: 34250718
Flagelin 22 purchased from MedChemExpress. Usage Cited in: Plant Biotechnol J. 2021 Nov;19(11):2319-2332. [Abstract]
The relative expression levels of the PTI‐ and MAPK‐related genes in response to flg22 in NIP plants at assessed by RT‐qPCR. NIP plants were infiltrated with 10 μM Flagelin 22 (flg22), and leaves were collected 30 min post‐infiltration for transcript analysis.
Flagelin 22 purchased from MedChemExpress. Usage Cited in: Plant Biotechnol J. 2021 Nov;19(11):2319-2332. [Abstract]
MAPK activation in NIP and osrlp1‐cas‐1# rice leaves after exogenous 10 μM Flagelin 22 (flg22) treatment for 0, 5, 10, 20 or 30 min. Anti‐p42/44 antibody was used for Western blotting.
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Mater Today Bio
Nanotube topography inhibits NLRP3 inflammasome activation by reducing microtubule glutamylation. [Abstract]2025 May 10:32:101838. PMID: 40487178 -
bioRxiv
2026 May 10:2026.05.04.722566. PMID: 42146548 -
Solvent & Solubility
H2O : 100 mg/mL (44.00 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
One milliliter of the female gametophytes is added to 100 mL sterilized seawater containing 1 μM of Flagelin 22, flg15, flg14, and flg22D43A, respectively. Controls are sterilized seawater and 0.1% BSA in sterilized seawater. Gametophytes are grown at 10°C with a 24-h photoperiod at 50 μmol photons m-2 s-1. Sterilized seawater medium is provided with 0.2 mM KNO3, 0.02mM KH2PO4, and 1 μM of the four respective peptides and refreshed every week. The gametophytes are briefly blotted dry, and the fresh weight is measured after 40 days[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[2]. Jiménez-Góngora T, et al. Flg22-Triggered Immunity Negatively Regulates Key BR Biosynthetic Genes. Front Plant Sci. 2015 Nov 9;6:981. [Content Brief]
[3]. Bethke G, et al. Flg22 regulates the release of an ethylene response factor substrate from MAP kinase 6 in Arabidopsis thaliana via ethylene signaling. Proc Natl Acad Sci U S A. 2009 May 12;106(19):8067-72. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.4400 mL | 2.2002 mL | 4.4005 mL | 11.0012 mL |
| 5 mM | 0.0880 mL | 0.4400 mL | 0.8801 mL | 2.2002 mL | |
| 10 mM | 0.0440 mL | 0.2200 mL | 0.4400 mL | 1.1001 mL | |
| 15 mM | 0.0293 mL | 0.1467 mL | 0.2934 mL | 0.7334 mL | |
| 20 mM | 0.0220 mL | 0.1100 mL | 0.2200 mL | 0.5501 mL | |
| 25 mM | 0.0176 mL | 0.0880 mL | 0.1760 mL | 0.4400 mL | |
| 30 mM | 0.0147 mL | 0.0733 mL | 0.1467 mL | 0.3667 mL | |
| 40 mM | 0.0110 mL | 0.0550 mL | 0.1100 mL | 0.2750 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Flagelin 22 Related Classifications
HY-P1568 Related Classifications
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