Flagelin 22 TFA
Based on 6 publication(s) in Google Scholar
Flagelin 22 TFA (Flagellin 22 TFA), a fragment of bacterial flagellin, is an effective elicitor in both plants and algae.
For research use only. We do not sell to patients.
- Purity: 99.92%
- Formula: C93H162N32O34.xC2HF3O2
- Molecular Weight:2272.48 (free base)
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Flagelin 22 TFA
More
Customer Validation & Images
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RT-PCR
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WB
Biological Activity
Flagelin 22 TFA (100 nM, 1 or 24 h) activates a gene that triggers immunity (PTI) and leads to reduced expression of growth-promoting hormone brassinolide (BR) biosynthesis in plants[2].
Flagelin 22 TFA (10 μM, 4 h) regulates the release of ethylene reactive factor substrate in Arabidopsis MAP kinase 6 by ethylene signaling[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 2272.48 (free base)
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Formula C93H162N32O34.xC2HF3O2
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Color White to off-white
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Synonyms
Flagellin 22 TFA
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Sequence
Gln-Arg-Leu-Ser-Thr-Gly-Ser-Arg-Ile-Asn-Ser-Ala-Lys-Asp-Asp-Ala-Ala-Gly-Leu-Gln-Ile-Ala
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Sequence Shortening
QRLSTGSRINSAKDDAAGLQIA
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (6)
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Journal Impact Factor
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Most Recent
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Cell
2025 Mar 6;188(5):1330-1348.e27. PMID: 39855200 -
Nat Commun
Lanthanum(III) triggers AtrbohD- and jasmonic acid-dependent systemic endocytosis in plants. [Abstract]2021 Jul 15;12(1):4327. PMID: 34267202 -
Plant Biotechnol J
A rice LRR receptor-like protein associates with its adaptor kinase OsSOBIR1 to mediate plant immunity against viral infection. [Abstract]2021 Nov;19(11):2319-2332. PMID: 34250718
Flagelin 22 TFA purchased from MedChemExpress. Usage Cited in: Plant Biotechnol J. 2021 Nov;19(11):2319-2332. [Abstract]
The relative expression levels of the PTI‐ and MAPK‐related genes in response to flg22 in NIP plants at assessed by RT‐qPCR. NIP plants were infiltrated with 10 μM Flagelin 22 (flg22), and leaves were collected 30 min post‐infiltration for transcript analysis.
Flagelin 22 TFA purchased from MedChemExpress. Usage Cited in: Plant Biotechnol J. 2021 Nov;19(11):2319-2332. [Abstract]
MAPK activation in NIP and osrlp1‐cas‐1# rice leaves after exogenous 10 μM Flagelin 22 (flg22) treatment for 0, 5, 10, 20 or 30 min. Anti‐p42/44 antibody was used for Western blotting.
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Mater Today Bio
Nanotube topography inhibits NLRP3 inflammasome activation by reducing microtubule glutamylation. [Abstract]2025 May 10:32:101838. PMID: 40487178 -
bioRxiv
2026 May 10:2026.05.04.722566. PMID: 42146548 -
Solvent & Solubility
DMSO : 100 mg/mL (Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 33.33 mg/mL (Need ultrasonic)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL; Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL; Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Protocol
One milliliter of the female gametophytes is added to 100 mL sterilized seawater containing 1 μM of Flagelin 22, flg15, flg14, and flg22D43A, respectively. Controls are sterilized seawater and 0.1% BSA in sterilized seawater. Gametophytes are grown at 10°C with a 24-h photoperiod at 50 μmol photons m-2 s-1. Sterilized seawater medium is provided with 0.2 mM KNO3, 0.02mM KH2PO4, and 1 μM of the four respective peptides and refreshed every week. The gametophytes are briefly blotted dry, and the fresh weight is measured after 40 days[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (280 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[2]. Jiménez-Góngora T, et al. Flg22-Triggered Immunity Negatively Regulates Key BR Biosynthetic Genes. Front Plant Sci. 2015 Nov 9;6:981. [Content Brief]
[3]. Bethke G, et al. Flg22 regulates the release of an ethylene response factor substrate from MAP kinase 6 in Arabidopsis thaliana via ethylene signaling. Proc Natl Acad Sci U S A. 2009 May 12;106(19):8067-72. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Flagelin 22 TFA Related Classifications
HY-P1568A Related Classifications
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