1. Anti-infection
  2. Bacterial
  3. Flagelin 22(TFA)

Flagelin 22(TFA) (Synonyms: Flagellin 22(TFA))

Cat. No.: HY-P1568A Purity: 99.39%
Handling Instructions

Flagelin 22 TFA (Flagellin 22 TFA), a fragment of bacterial flagellin, is an effective elicitor in both plants and algae.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Flagelin 22(TFA) Chemical Structure

Flagelin 22(TFA) Chemical Structure

Size Price Stock Quantity
10 mM * 1 mL in Water USD 1601 In-stock
Estimated Time of Arrival: December 31
1 mg USD 120 In-stock
Estimated Time of Arrival: December 31
5 mg USD 390 In-stock
Estimated Time of Arrival: December 31
10 mg USD 610 In-stock
Estimated Time of Arrival: December 31
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100 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

Other Forms of Flagelin 22(TFA):

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Description

Flagelin 22 TFA (Flagellin 22 TFA), a fragment of bacterial flagellin, is an effective elicitor in both plants and algae.

In Vitro

Flagelin 22 (flg22) is a 22-amino-acid peptide, which corresponds to the highly conserved N-terminal region of flagellin, can induce immunity reaction in various plants such as tomato (Solanum lycopersicum), potato (Solanum tuberosum), tobacco (Nicotiana tabacum), and Arabidopsis thaliana. Flagelin 22 can induce oxidative bursts and hypersensitive responses (HR) in both female gametophytes and sporophytes of Saccharina japonica, indicating that algae and plants may share similar mechanisms for recognizing pathogens. After culturing the female gametophytes of S. japonica in the presence of Flagelin 22, flg15, flg14, and flg22D43A for 40 days, both Flagelin 22 and flg15 significantly induce growth inhibition of the algae at a concentration of 1 μM. The fresh weights of Flagelin 22- and flg15-challenged female gametophytes are less than one half of the control[1].

Molecular Weight

2386.50

Formula

C₉₅H₁₆₃F₃N₃₂O₃₆

Shipping

Room temperature in continental US; may vary elsewhere

Storage
Powder -80°C 2 years
  -20°C 1 year
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : ≥ 50 mg/mL (20.95 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.4190 mL 2.0951 mL 4.1902 mL
5 mM 0.0838 mL 0.4190 mL 0.8380 mL
10 mM 0.0419 mL 0.2095 mL 0.4190 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

One milliliter of the female gametophytes is added to 100 mL sterilized seawater containing 1 μM of Flagelin 22, flg15, flg14, and flg22D43A, respectively. Controls are sterilized seawater and 0.1% BSA in sterilized seawater. Gametophytes are grown at 10°C with a 24-h photoperiod at 50 μmol photons m-2 s-1. Sterilized seawater medium is provided with 0.2 mM KNO3, 0.02mM KH2PO4, and 1 μM of the four respective peptides and refreshed every week. The gametophytes are briefly blotted dry, and the fresh weight is measured after 40 days[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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Flagelin 22(TFA)
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HY-P1568A
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