2. Anti-infection
  3. Bacterial
  4. Flagelin 22 TFA

Flagelin 22 TFA  (Synonyms: Flagellin 22 TFA)

Cat. No.: HY-P1568A Purity: 99.92%
COA
SDS
Handling Instructions Technical Support

Flagelin 22 TFA (Flagellin 22 TFA), a fragment of bacterial flagellin, is an effective elicitor in both plants and algae.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

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Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of Flagelin 22 TFA:

Flagelin 22 TFA Related Antibodies

Top Publications Citing Use of Products

    Flagelin 22 TFA purchased from MedChemExpress. Usage Cited in: Plant Biotechnol J. 2021 Nov;19(11):2319-2332.  [Abstract]

    The relative expression levels of the PTI‐ and MAPK‐related genes in response to flg22 in NIP plants at assessed by RT‐qPCR. NIP plants were infiltrated with 10 μM Flagelin 22 (flg22), and leaves were collected 30 min post‐infiltration for transcript analysis.

    Flagelin 22 TFA purchased from MedChemExpress. Usage Cited in: Plant Biotechnol J. 2021 Nov;19(11):2319-2332.  [Abstract]

    MAPK activation in NIP and osrlp1‐cas‐1# rice leaves after exogenous 10 μM Flagelin 22 (flg22) treatment for 0, 5, 10, 20 or 30 min. Anti‐p42/44 antibody was used for Western blotting.

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Flagelin 22 TFA (Flagellin 22 TFA), a fragment of bacterial flagellin, is an effective elicitor in both plants and algae[1][2][3].

    In Vitro

    Flagelin 22 TFA (100 nM, 1 or 24 h) activates a gene that triggers immunity (PTI) and leads to reduced expression of growth-promoting hormone brassinolide (BR) biosynthesis in plants[2].
    Flagelin 22 TFA (10 μM, 4 h) regulates the release of ethylene reactive factor substrate in Arabidopsis MAP kinase 6 by ethylene signaling[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Flagelin 22 TFA Related Antibodies
    Molecular Weight

    2272.48 (free base)

    Formula

    C93H162N32O34.xC2HF3O2
    Appearance

    Solid

    Color

    White to off-white

    Sequence

    Gln-Arg-Leu-Ser-Thr-Gly-Ser-Arg-Ile-Asn-Ser-Ala-Lys-Asp-Asp-Ala-Ala-Gly-Leu-Gln-Ile-Ala
    Sequence Shortening

    QRLSTGSRINSAKDDAAGLQIA
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Sealed storage, away from moisture

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 33.33 mg/mL (Need ultrasonic)

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

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    C2

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL; Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL; Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.92%

    SDS (252 KB)

    COA (254 KB) RP-HPLC (245 KB) MS (72 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [1]

    One milliliter of the female gametophytes is added to 100 mL sterilized seawater containing 1 μM of Flagelin 22, flg15, flg14, and flg22D43A, respectively. Controls are sterilized seawater and 0.1% BSA in sterilized seawater. Gametophytes are grown at 10°C with a 24-h photoperiod at 50 μmol photons m-2 s-1. Sterilized seawater medium is provided with 0.2 mM KNO3, 0.02mM KH2PO4, and 1 μM of the four respective peptides and refreshed every week. The gametophytes are briefly blotted dry, and the fresh weight is measured after 40 days[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References
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    Flagelin 22 TFA Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Flagelin 22Flagellin 22Flagelin22Flagelin-22Flagellin22Flagellin-22BacterialFlg22PTIBRMAPK6Plant InducersInhibitorinhibitorinhibit

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