2. Metabolic Enzyme/Protease Autophagy
  3. FXR Autophagy
  4. Gly-β-MCA

Gly-β-MCA, a bile acid, is a potent, sable, intestine-selective and oral bioactive farnesoid X receptor (FXR) inhibitor that may be a candidate for the treatment of metabolic disorders.

For research use only. We do not sell to patients.

CAS No. : 66225-78-3

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Customer Review

Based on 23 publication(s) in Google Scholar

Other Forms of Gly-β-MCA:

Gly-β-MCA Related Antibodies

Top Publications Citing Use of Products

    Gly-β-MCA purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Dec 31:e2412071.  [Abstract]

    RT‐qPCR analysis of Wtap and Rorc mRNA levels in rat intestinal γδT cells and ILC3s with with HCA, Gly-β-MCA (10 μM; 16 h), GW4064 treatment.

    Gly-β-MCA purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 Aug 12;42(8):1386-1400.e8.  [Abstract]

    Cell viability at OD570 of allocholic acid-treated CRC cells after using FXR1 antagonist glycine-β-muricholic acid (50 μM).

    Gly-β-MCA purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 Aug 12;42(8):1386-1400.e8.  [Abstract]

    Gly-β-MCA (50 μM). Protein expression of cell proliferation, cell cycle, or MAPK pathway markers in CRC cells.

    Gly-β-MCA purchased from MedChemExpress. Usage Cited in: Microbiome. 2024 Feb 5;12(1):20.  [Abstract]

    The expression of SLA-I was analyzed by flow cytometry in the presence and absence of SBI-115 and Gly-β-MCA (2 μM) in IPEC-J2 cells treated with 20 μM LCA for 12 h.

    Gly-β-MCA purchased from MedChemExpress. Usage Cited in: Cell Metab. 2023 Oct 3;35(10):1752-1766.e8.  [Abstract]

    Gly-β-MCA (10 mg/kg). Protein expression of CYP7A1 and CYP7B1 in the liver.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Gly-β-MCA, a bile acid, is a potent, sable, intestine-selective and oral bioactive farnesoid X receptor (FXR) inhibitor that may be a candidate for the treatment of metabolic disorders[1].

    IC50 & Target

    FXR[1].
    In Vitro

    Gly-β-MCA, a bile acid, is a potent, sable and intestine-selective and farnesoid X receptor (FXR) inhibitor[1].
    Gly-β-MCA (Gly-MCA, ) is resistant to hydrolysis by BSH[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Gly-β-MCA Related Antibodies
    In Vivo

    Gly-β-MCA (Gly-MCA, p.o. 10 and 50 mg/kg) prevents and treats diet-induced and genetic obesity, along with insulin resistance and hepatic steatosis without systemic, hepatic or intestinal toxicities in mice[1].
    Gly-MCA does not increase faecal LCN-2 levels, indicating that Gly-MCA does not induce intestinal inflammation[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    465.62

    Formula

    C26H43NO6
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    C[C@@H]([C@@]1([H])CC[C@@]2([H])[C@@]3([H])[C@]([C@]4(CC[C@@H](O)C[C@H]4[C@H](O)[C@@H]3O)C)([H])CC[C@]12C)CCC(NCC(O)=O)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (214.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.1477 mL 10.7384 mL 21.4767 mL
    5 mM 0.4295 mL 2.1477 mL 4.2953 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (4.47 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (4.47 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.95%

    SDS (393 KB)

    COA (251 KB) HNMR (148 KB) RP-HPLC (208 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.1477 mL 10.7384 mL 21.4767 mL 53.6919 mL
    5 mM 0.4295 mL 2.1477 mL 4.2953 mL 10.7384 mL
    10 mM 0.2148 mL 1.0738 mL 2.1477 mL 5.3692 mL
    15 mM 0.1432 mL 0.7159 mL 1.4318 mL 3.5795 mL
    20 mM 0.1074 mL 0.5369 mL 1.0738 mL 2.6846 mL
    25 mM 0.0859 mL 0.4295 mL 0.8591 mL 2.1477 mL
    30 mM 0.0716 mL 0.3579 mL 0.7159 mL 1.7897 mL
    40 mM 0.0537 mL 0.2685 mL 0.5369 mL 1.3423 mL
    50 mM 0.0430 mL 0.2148 mL 0.4295 mL 1.0738 mL
    60 mM 0.0358 mL 0.1790 mL 0.3579 mL 0.8949 mL
    80 mM 0.0268 mL 0.1342 mL 0.2685 mL 0.6711 mL
    100 mM 0.0215 mL 0.1074 mL 0.2148 mL 0.5369 mL
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    Gly-β-MCA Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Gly-β-MCA66225-78-3FXRAutophagyNR1H4Inhibitorinhibitorinhibit

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