HTBA
Based on 1 Customer Validation
HTBA (3-Hydroxy-2,4,6-triiodobenzoic acid) is a biochemical reagent and important intermediate. HTBA can be used in the research of life science, organic chemistry and other fields.
For research use only. We do not sell to patients.
- Purity: 99.67%
- CAS No.: 53279-72-4
- Formula: C7H3I3O3
- Molecular Weight:515.81
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
HTBA can be used as an indicator for the detection of 5'-nucleotidase. Compared with the traditional phenol system, it is more sensitive and less likely to denature proteins, and the absorbance can be read at 520 nm[1].
HTBA can be used as a chromogenic substrate for lipid quantification. In the sequential microenzymatic assay of cholesterol, triglycerides, and phospholipids, it undergoes oxidative condensation with 4-aminoantipyrine under the catalysis of horseradish peroxidase to generate a pink dye for the detection of hydrogen peroxide[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 53279-72-4
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Appearance Solid
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Molecular Weight 515.81
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Formula C7H3I3O3
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Color Light brown to yellow
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SMILES
O=C(O)C1=C(I)C=C(I)C(O)=C1I
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Synonyms
3-Hydroxy-2,4,6-triiodobenzoic acid
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Solvent & Solubility
DMSO : ≥ 33 mg/mL (63.98 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.03 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.03 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (617 KB)
- English - EN (617 KB)
- Français - FR (617 KB)
- Deutsch - DE (617 KB)
- Norwegian - NO (617 KB)
- Español - ES (617 KB)
- Swedish - SV (617 KB)
- Italian - IT (617 KB)
- Korean - KR (617 KB)
- Portuguese - PT (617 KB)
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Handling Instructions (2659 KB)
References
[1]. Iob A, et al. Uricase immobilized on walls of glass tubes used as reactors in continuous-flow analyses. Clin Chem. 1981 Jan;27(1):195-6. [Content Brief]
[2]. Nanjee MN, et al. Sequential microenzymatic assay of cholesterol, triglycerides, and phospholipids in a single aliquot. Clin Chem. 1996 Jun;42(6 Pt 1):915-26. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.9387 mL | 9.6935 mL | 19.3870 mL | 48.4675 mL |
| 5 mM | 0.3877 mL | 1.9387 mL | 3.8774 mL | 9.6935 mL | |
| 10 mM | 0.1939 mL | 0.9693 mL | 1.9387 mL | 4.8467 mL | |
| 15 mM | 0.1292 mL | 0.6462 mL | 1.2925 mL | 3.2312 mL | |
| 20 mM | 0.0969 mL | 0.4847 mL | 0.9693 mL | 2.4234 mL | |
| 25 mM | 0.0775 mL | 0.3877 mL | 0.7755 mL | 1.9387 mL | |
| 30 mM | 0.0646 mL | 0.3231 mL | 0.6462 mL | 1.6156 mL | |
| 40 mM | 0.0485 mL | 0.2423 mL | 0.4847 mL | 1.2117 mL | |
| 50 mM | 0.0388 mL | 0.1939 mL | 0.3877 mL | 0.9693 mL | |
| 60 mM | 0.0323 mL | 0.1616 mL | 0.3231 mL | 0.8078 mL |
HTBA Related Classifications
HY-15919 Related Classifications
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