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KRN2 bromide 

Cat. No.: HY-112125A Purity: >98.0%
Handling Instructions

KRN2 is a selective inhibitor of nuclear factor of activated T cells (NFAT5), with an IC50 of 0.1 μM.

For research use only. We do not sell to patients.

KRN2 bromide Chemical Structure

KRN2 bromide Chemical Structure

CAS No. : 1390654-28-0

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10 mM * 1 mL in DMSO USD 254 In-stock
Estimated Time of Arrival: December 31
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25 mg USD 750 In-stock
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50 mg USD 1200 In-stock
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100 mg USD 2100 In-stock
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Other Forms of KRN2 bromide:

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

KRN2 is a selective inhibitor of nuclear factor of activated T cells (NFAT5), with an IC50 of 0.1 μM.

IC50 & Target

IC50: 0.1 μM (NFAT5)[1]

In Vitro

KRN2 shows much stronger inhibition of NFAT5-dependent reporter activity in RAW 264.7 macrophages than does BBR; the IC50 value is 0.1 μM for KRN2 and 4 μM for BBR. In parallel, 0.5 μM of KRN2 significantly suppresses the LPS-stimulated increase in NFAT5 protein expression in RAW 264.7 macrophages. It is confirmed that LPS-induced NFAT5 mRNA and protein expression is nearly completely blocked by KRN2. Similarly, KRN2 inhibits the translocation of NFAT5 into the nucleus of RAW264.7 cells stimulated with LPS. KRN2 specifically represses LPS-induced NFAT5 promoter activity, whereas it fails to reduce high salt-induced NFAT5 activity in the same cells, which is consistent with the selective inhibition of TLR4-activated NFAT5, but not hypertonicity-induced NFAT5, by KRN2[1].

Solvent & Solubility
In Vitro: 

DMSO : ≥ 60 mg/mL (114.42 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9070 mL 9.5351 mL 19.0701 mL
5 mM 0.3814 mL 1.9070 mL 3.8140 mL
10 mM 0.1907 mL 0.9535 mL 1.9070 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

ROS production by RAW 264.7 cells stimulates with LPS (1 µg/mL) in the presence or absence of KRN2 (0.8 µM). ROS expression is determined by flow cytometry using a ROS probe. KRN2 (0.8 µM) is added to RAW 264.7 cells, which are then stimulated with LPS (1 µg/mL) in a time-dependent manner. Activity (%) of p38 isoform is measured in stable cell lines (CHO) in the presence of KRN2 or BBR, using a promoter reporter assay. Cells are treated with KRN2 in various doses (0.5, 1, 2, 5 μM) before stimulation with LPS (1 µg/mL) for 5 minutes and then fractionated into cytosolic extracts (upper panel) and nuclear extracts (lower panel)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

524.38

Formula

C₂₇H₂₃BrFNO₄

CAS No.

1390654-28-0

SMILES

COC1=C(OC)C2=C[N+]3=C(C(C(CC3)=C4)=CC5=C4OCO5)C(CC6=CC=CC=C6F)=C2C=C1.[Br-]

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
KRN2 bromide
Cat. No.:
HY-112125A
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KRN2 bromide

Cat. No.: HY-112125A