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    Apoptosis
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  3. NSC 23766 trihydrochloride

NSC 23766 trihydrochloride 

Cat. No.: HY-15723A Purity: 99.66%
Handling Instructions

NSC 23766 trihydrochloride is an inhibitor of Rac1 activation.

For research use only. We do not sell to patients.

NSC 23766 trihydrochloride Chemical Structure

NSC 23766 trihydrochloride Chemical Structure

CAS No. : 1177865-17-6

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Free Sample (0.5-1 mg)   Apply Now  
10 mM * 1 mL in DMSO USD 70 In-stock
Estimated Time of Arrival: December 31
5 mg USD 60 In-stock
Estimated Time of Arrival: December 31
10 mg USD 106 In-stock
Estimated Time of Arrival: December 31
50 mg USD 451 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of NSC 23766 trihydrochloride:

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Description

NSC 23766 trihydrochloride is an inhibitor of Rac1 activation.

In Vitro

NSC 23766 (100 μM) treatment effectively inhibits polar body emission in a dose-dependent manner. NSC 23766 (200 μM) increases the percentage of morphologically abnormal spindles of oocytes. In NSC 23766-treated oocytes, the p-MAPK protein expression is significantly decreased[2]. NSC23766 (50 μM) plus 100 ng/mL Jagged1, GDF9 and BMP15, reduces the number of germLine cell cysts and increases the number of primordial follicles[3]. NSC23766 significantly inhibits GTP-Rac1 activity and phosphorylation of Rac1-PAK, ERKs and p38 MAPK in the spinal dorsal horn neurons[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

NSC23766 (2.5 mg/kg/day, i.p.) significantly attenuates the onset of spontaneous diabetes in NOD mice, without significant effects on the growth (body weights) of the mice. NSC23766 significantly increases the expression of Rac1 and CHOP, a marker for ER-stress, in islets from NOD mice[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

530.96

Formula

C₂₄H₃₈Cl₃N₇

CAS No.

1177865-17-6

SMILES

NC1=CC(C)=NC2=CC=C(NC3=NC(NC(C)CCCN(CC)CC)=NC(C)=C3)C=C12.[H]Cl.[H]Cl.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 33.33 mg/mL (62.77 mM; Need ultrasonic)

H2O : ≥ 32 mg/mL (60.27 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8834 mL 9.4169 mL 18.8338 mL
5 mM 0.3767 mL 1.8834 mL 3.7668 mL
10 mM 0.1883 mL 0.9417 mL 1.8834 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (4.71 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (4.71 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Kinase Assay
[4]

Briefly, fresh spinal cord tissue of the lumbar enlargement is homogenised in the presence of protease and phosphatase inhibitors and lysed with buffer. After being centrifuged at 12,000× g for 5 min at 4°C, the supernatants are collected and incubated with PAK-PBD beads at 4°C on a rotator for 1 h and then the beads are pelleted through centrifugation at 5000× g for 3 min at 4°C. The resulting pellet is resuspended in LaemmLi buffer and boiled for 2 min. The bead samples are subjected to Western blot analysis. Total Rac1 in each sample is also determined by Western blot analysis.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

Balb/c control and NOD mice are at 7 weeks of age and are divided into four groups (n=8/group). At 8 weeks of age two groups of experimental animals (Balb/c and NOD) receive NSC23766 (2.5 mg/kg/day, i.p./daily) and other two groups, which serve as control Balb/c and NOD mice and receive equal volume of saline. The body weights and blood glucose are monitored every week for 34 weeks.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: 99.66%

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Keywords:

NSC 23766 trihydrochlorideRasApoptosisInhibitorinhibitorinhibit

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NSC 23766 trihydrochloride
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