Neostigmine (methyl sulfate)

Name: Neostigmine (methyl sulfate)
Brand: MedChemExpress
SKU: HY-B1206
Rating: 4.5 (1 reviews)

Cat. No.: HY-B1206 Purity: 99.50%: Data Sheet
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Neostigmine methyl sulfate is an AchE inhibitor. Neostigmine methyl sulfate increases endogenous acetylcholine levels, mediates antinociceptive effects, and alleviates heat-induced pain. Neostigmine methyl sulfate can be used in studies related to postoperative pain and sepsis.

For research use only. We do not sell to patients.

Neostigmine (methyl sulfate) Chemical Structure

CAS No. : 51-60-5

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Neostigmine (methyl sulfate):

Neostigmine Bromide In-stock
Neostigmine methyl sulfate (Standard) In-stock

1 Publications Citing Use of MCE Neostigmine (methyl sulfate)

•bioRxiv. 2024 June 12.

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Purity & Documentation
References
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Description

In Vivo

Neostigmine methyl sulfate (intrathecal) potently inhibits postoperative mechanical hyperalgesia, reduces thermally evoked pain, and acts synergistically with intrathecal morphine in Rattus norvegicus^[1].
Neostigmine methyl sulfate (2-20 µg; single dose or cumulative 3 doses, 24 hours apart) induces statistically insignificant mitodepression and no significant chromosome aberrations in the bone marrow cells of 8-10 week old Swiss albino mice, indicating it is not genotoxic in this in vivo model^[2].
Neostigmine methyl sulfate (0.135 mg/kg; i.p.; single dose) administered prior to E. coli-induced sepsis reduces mouse mortality by 17.5% at 6 hours and 30.0% at 24 hours, and significantly lowers circulating proinflammatory cytokine concentrations in a preclinical mouse model of sepsis^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Mongrel white mice (both sexes, 18-22g, sepsis modeled via i.p. injection of E. coli O157:H7)^[3]
Dosage:	0.135 mg/kg
Administration:	i.p.; single dose
Result:	Reduced 6-hour mortality to 18.9% and 24-hour mortality to 59.4%. Reduced 6-hour blood TNF-α concentration to 155 pg/mL. Reduced 6-hour blood IL-1β concentration to 199 pg/mL. Reduced 6-hour blood IL-6 concentration to 268 pg/mL. Reduced 24-hour blood TNF-α concentration to 57 pg/mL. Reduced 24-hour blood IL-1β concentration to 57 pg/mL. Reduced 24-hour blood IL-6 concentration to 80 pg/mL.

Molecular Weight

334.39

Formula

C₁₃H₂₂N₂O₆S

CAS No.

51-60-5

Appearance

Solid

Color

White to off-white

SMILES

C[N+](C)(C)C1=CC=CC(OC(N(C)C)=O)=C1.O=S(OC)([O-])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (299.05 mM; Need ultrasonic)

DMSO : ≥ 100 mg/mL (299.05 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9905 mL	14.9526 mL	29.9052 mL
5 mM	0.5981 mL	2.9905 mL	5.9810 mL
10 mM	0.2991 mL	1.4953 mL	2.9905 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.48 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.48 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.48 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (299.05 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.50%

Select Batch:

Data Sheet (280 KB) SDS (584 KB)

COA (253 KB) LCMS (96 KB)

Handling Instructions (2659 KB)

References

[1]. Buvanendran A, et al. Useful adjuvants for postoperative pain management. Best Pract Res Clin Anaesthesiol. 2007;21(1):31-49.

[2]. Rathna Prabha, et al. GENOTOXICITY OF BLOXIVERZ (NEOSTIGMINE METHYLSULFATE) ON BONE MARROW CELLS OF MOUSE. ejpmr, 2016,3(7), 263-269.

[3]. Zabrodskii PF, et al. The Influence of Various Drags on Mortality of Mice and the Concentration of Proinflammatory Cytokines in Blood at Sepsis Caused by E. Coli. Adv Case Stud. 1(3).

[4]. Mokaram Dori M, et al. The Analgesic Efficacy of Intrathecal Bupivacaine and Fentanyl with Added Neostigmine or Magnesium Sulphate. Anesth Pain Med. 2016;6(6):e9651. Published 2016 Sep 18.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.9905 mL	14.9526 mL	29.9052 mL	74.7630 mL
	5 mM	0.5981 mL	2.9905 mL	5.9810 mL	14.9526 mL
	10 mM	0.2991 mL	1.4953 mL	2.9905 mL	7.4763 mL
	15 mM	0.1994 mL	0.9968 mL	1.9937 mL	4.9842 mL
	20 mM	0.1495 mL	0.7476 mL	1.4953 mL	3.7381 mL
	25 mM	0.1196 mL	0.5981 mL	1.1962 mL	2.9905 mL
	30 mM	0.0997 mL	0.4984 mL	0.9968 mL	2.4921 mL
	40 mM	0.0748 mL	0.3738 mL	0.7476 mL	1.8691 mL
	50 mM	0.0598 mL	0.2991 mL	0.5981 mL	1.4953 mL
	60 mM	0.0498 mL	0.2492 mL	0.4984 mL	1.2460 mL
	80 mM	0.0374 mL	0.1869 mL	0.3738 mL	0.9345 mL
	100 mM	0.0299 mL	0.1495 mL	0.2991 mL	0.7476 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.