Nitromifene (Synonyms: CI628)

Cat. No.: HY-100266: FAQs
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Nitromifene is an antagonist of estrogen receptor (ER).

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Nitromifene Chemical Structure

CAS No. : 10448-84-7

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Purity & Documentation
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Description	Nitromifene is an antagonist of estrogen receptor (ER).
IC₅₀ & Target	ER^[1]
In Vitro	Nitromifene is an antagonist of estrogen receptor (ER). At 0.5 μM and 1.0 μM concentrations, Nitromifene inhibits MCF 7 cell proliferation to 70% of that in drug-free controls. At higher concentrations, Nitromifene is clearly more effective than other metabolites. Specifically bound estradiol is displaced from intact MCF 7 cells by Nitromifene. Nitromifene is an effective antagonist of the ability of calmodulin (CM) to activate cyclic nucleotide phosphodiesterase^[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Nitromifene Related Antibodies
Molecular Weight	444.52
Formula	C₂₇H₂₈N₂O₄
CAS No.	10448-84-7
SMILES	O=[N+](/C(C1=CC=CC=C1)=C(C2=CC=C(OCCN3CCCC3)C=C2)/C4=CC=C(OC)C=C4)[O-]
Shipping	Room temperature in continental US; may vary elsewhere.
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation	Data Sheet (269 KB) Handling Instructions (2659 KB)
References	[1]. Ruenitz PC, et al. Characterization of MCF 7 breast cancer cell growth inhibition by the antiestrogen nitromifene (CI 628) and selected metabolites. J Steroid Biochem. 1989 Sep;33(3):365-9. [Content Brief]

Cell Assay

MCF 7 cells are used in this study. The experiment is carried out in 6-well cluster plates. To each well is added 5 mL of a suspension of 2×10⁵ cells in log phase growth. Medium is changed after 48 h and after 96 h, medium is replaced with 5 mL of serum-free medium containing 2 nM of the radioligand. Nitromifene is dissolved in DMSO (5 μL) to give final concentrations of 0, 0.01, 0.03, 0.1, 0.3, 1, 3, and 10 μM. Nonspecific binding is determined in incubations to which the radioinert counterpart of the radioligand is added at a concentration of 1 μM. Incubations are run in triplicate for 1 h at 37°C.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Ruenitz PC, et al. Characterization of MCF 7 breast cancer cell growth inhibition by the antiestrogen nitromifene (CI 628) and selected metabolites. J Steroid Biochem. 1989 Sep;33(3):365-9. [Content Brief]

Nitromifene Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Nitromifene10448-84-7CI628CI 628CI-628Estrogen Receptor/ERRInhibitorinhibitorinhibit

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Nitromifene (Synonyms: CI628)

Nitromifene Related Antibodies