2. Metabolic Enzyme/Protease
  3. 15-PGDH
  4. SW033291

SW033291 is a potent and high-affinity inhibitor of 15-PGDH with a Ki of 0.1 nM. SW033291 increases prostaglandin PGE2 levels in bone marrow and other tissues. SW033291 also promotes tissue regeneration.

For research use only. We do not sell to patients.

CAS No. : 459147-39-8

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10 mM * 1 mL in DMSO
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Customer Review

Based on 7 publication(s) in Google Scholar

Other Forms of SW033291:

SW033291 Related Antibodies

Top Publications Citing Use of Products

    SW033291 purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2020 Feb 21;11(1):76.   [Abstract]

    Cell viability of MDSCs in GM under different concentration of SW033291 (20, 50, 100, 500, 1000 nM) treatment.

    SW033291 purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2020 Feb 21;11(1):76.   [Abstract]

    LIVE/DEAD staining of MDSCs cultured in DM and GM treated with different concentration of SW033291 (20, 50, 100, 500, 1000 nM) on day 1 and day 3.

    SW033291 purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2020 Feb 21;11(1):76.   [Abstract]

    qPCR analysis of the expression of myogenic differentiation-related genes in MDSCs cultured in DM (top row) and GM (bottom row) under 100 nM SW033291 treatment on days 3 and 7.

    SW033291 purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2020 Feb 21;11(1):76.   [Abstract]

    Western blot analysis of the protein expression of myogenic markers in MDSCs cultured in DM and GM under 100 nM SW033291 treatment on day 3.

    SW033291 purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2020 Feb 21;11(1):76.   [Abstract]

    Cellular immunochemistry image of MHC in MDSCs cultured in DM and GM under 100 nM SW033291 treatment on day 7.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    SW033291 is a potent and high-affinity inhibitor of 15-PGDH with a Ki of 0.1 nM. SW033291 increases prostaglandin PGE2 levels in bone marrow and other tissues. SW033291 also promotes tissue regeneration[1].

    IC50 & Target

    Ki: 0.1 nM (15-PGDH)[1]
    In Vitro

    Treating cells with SW033291 decreases cellular 15-PGDH enzyme activity by 85%. SW033291 inhibition of 15-PGDH was non-competitive versus PGE2 over concentrations up to 40 μM PGE2. Treatment of A549 cells with SW033291 increases PGE2 levels by 3.5-fold at 500 nM, with an EC50 50 at approximately 75 nM[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    SW033291 Related Antibodies
    In Vivo

    SW033291 (10 mg/kg; intraperitoneal injection; twice daily; for 3 days; C57BL/6J mice) treatment for three consecutive days shows significant benefits, including a doubling of peripheral neutrophil counts, a 65% increase in marrow SKL cells, and a 71% increase in marrow SLAM cells[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: C57BL/6J mice[1]
    Dosage: 10 mg/kg
    Administration: Intraperitoneal injection; twice daily; for 3 days ( for 5 doses)
    Result: Showed significant benefits, including a doubling of peripheral neutrophil counts, a 65% increase in marrow SKL cells, and a 71% increase in marrow SLAM cells.
    Molecular Weight

    412.59

    Formula

    C21H20N2OS3
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    NC1=C(S(CCCC)=O)SC2=NC(C3=CC=CS3)=CC(C4=CC=CC=C4)=C21
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 33.33 mg/mL (80.78 mM; ultrasonic and warming and heat to 70°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Ethanol : 25 mg/mL (60.59 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.4237 mL 12.1186 mL 24.2371 mL
    5 mM 0.4847 mL 2.4237 mL 4.8474 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: 2.5 mg/mL (6.06 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% EtOH    40% PEG300    5% Tween-80    45% Saline

      Solubility: 2.5 mg/mL (6.06 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.36%

    SDS (393 KB)

    COA (251 KB) HNMR (270 KB) RP-HPLC (260 KB) MS (229 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    Ethanol / DMSO 1 mM 2.4237 mL 12.1186 mL 24.2371 mL 60.5928 mL
    5 mM 0.4847 mL 2.4237 mL 4.8474 mL 12.1186 mL
    10 mM 0.2424 mL 1.2119 mL 2.4237 mL 6.0593 mL
    15 mM 0.1616 mL 0.8079 mL 1.6158 mL 4.0395 mL
    20 mM 0.1212 mL 0.6059 mL 1.2119 mL 3.0296 mL
    25 mM 0.0969 mL 0.4847 mL 0.9695 mL 2.4237 mL
    30 mM 0.0808 mL 0.4040 mL 0.8079 mL 2.0198 mL
    40 mM 0.0606 mL 0.3030 mL 0.6059 mL 1.5148 mL
    50 mM 0.0485 mL 0.2424 mL 0.4847 mL 1.2119 mL
    60 mM 0.0404 mL 0.2020 mL 0.4040 mL 1.0099 mL
    DMSO 80 mM 0.0303 mL 0.1515 mL 0.3030 mL 0.7574 mL
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    SW033291 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    SW033291459147-39-8SW 033291SW-03329115-PGDH15-Hydroxyprostaglandin dehydrogenasePeripheralneutrophilknockoutprostaglandinPGE2hematopoieticbonemarrowInhibitorinhibitorinhibit

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