1. Metabolic Enzyme/Protease
  2. Phosphatase
  3. TCS 401

TCS 401 

Cat. No.: HY-12312 Purity: >98.0%
Handling Instructions

TCS 401 is a selective inhibitor of protein tyrosine phosphatase 1B (PTP1B).

For research use only. We do not sell to patients.

TCS 401 Chemical Structure

TCS 401 Chemical Structure

CAS No. : 243966-09-8

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Free Sample (0.5-1 mg)   Apply Now  
10 mM * 1  mL in DMSO USD 145 In-stock
Estimated Time of Arrival: December 31
5 mg USD 132 In-stock
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10 mg USD 204 In-stock
Estimated Time of Arrival: December 31
50 mg USD 660 In-stock
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100 mg USD 1080 In-stock
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Customer Review

Based on 3 publication(s) in Google Scholar

Top Publications Citing Use of Products

    TCS 401 purchased from MCE. Usage Cited in: J Tissue Eng Regen Med. 2020 Feb 15.

    The activation of Src by treatment with the 152RM peptide is reversed by pretreatment with the PTP1B inhibitor TCS-401, and the increased phosphorylation of β-catenin Y654 by treatment with the 152RM peptide is reduced by pretreatment with the Src inhibitor bosutinib.
    • Biological Activity

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    • Customer Review

    Description

    TCS 401 is a selective inhibitor of protein tyrosine phosphatase 1B (PTP1B).

    In Vitro

    TCS-401 (0.5, 1, 2 μM) significantly increases the proliferation of RPE cells. TCS-401 significantly increases the expression of cyclin A and cyclin D1 at the concentrations of 1 and 2 μM in a concentration-dependent manner. TCS-401 at concentrations of 0.5, 1, and 2 μM significantly increases phosphorylation of Erk and Akt compared to the control group. The activation of Erk and Akt by TCS-401 is blocked by pretreatment with PD98059 and LY294002, respectively. CS-401 treatment activates the MEK/Erk and PI3K/Akt signaling pathways and induces proliferation, differentiation, and migration in RPE cells[1]. CS-401 dose dependently inhibits the RPTC-Sup-induced reduction of fibronectin and α-SMA. At 1 μM, TCS-401 reverses the levels of fibronectin and α-SMA about onefold and at a dose of 2 μM, TCS-401 brings back fibronectin and α-SMA expression to near normal levels[2].

    Molecular Weight

    306.72

    Formula

    C₁₀H₁₁ClN₂O₅S

    CAS No.

    243966-09-8

    SMILES

    O=C(C1=C(NC(C(O)=O)=O)SC2=C1CCNC2)O.[H]Cl

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 11 mg/mL (35.86 mM; Need ultrasonic and warming)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.2603 mL 16.3015 mL 32.6030 mL
    5 mM 0.6521 mL 3.2603 mL 6.5206 mL
    10 mM 0.3260 mL 1.6302 mL 3.2603 mL
    *Please refer to the solubility information to select the appropriate solvent.
    References
    Cell Assay
    [1]

    Cell proliferation are examined using MTT assay. The 5×103 cells grown in a 96-well plate for 24 h are partially starved in DMEM/F12 supplemented with 1% FBS for 12 h, and then stimulated with various concentrations of TCS-401 for an additional 24 h. MTT are added to the culture medium, and the cells are incubated for an additional 4 h. The formazan crystals formed are then dissolved by adding dimethyl sulfoxide (100 μL per well). Absorbance at 490 nm are measured using a microplate reader.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
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    Keywords:

    TCS 401TCS401TCS-401PhosphataseInhibitorinhibitorinhibit

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