1. Metabolic Enzyme/Protease
  2. Phosphatase

TCS 401 

Cat. No.: HY-12312 Purity: >98.0%
Data Sheet SDS Handling Instructions

TCS 401 is a selective inhibitor of protein tyrosine phosphatase 1B (PTP1B).

For research use only. We do not sell to patients.
TCS 401 Chemical Structure

TCS 401 Chemical Structure

CAS No. : 243966-09-8

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO $121 In-stock
5 mg $110 In-stock
10 mg $170 In-stock
50 mg $550 In-stock
100 mg $900 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References


TCS 401 is a selective inhibitor of protein tyrosine phosphatase 1B (PTP1B).

In Vitro

TCS-401 (0.5, 1, 2 μM) significantly increases the proliferation of RPE cells. TCS-401 significantly increases the expression of cyclin A and cyclin D1 at the concentrations of 1 and 2 μM in a concentration-dependent manner. TCS-401 at concentrations of 0.5, 1, and 2 μM significantly increases phosphorylation of Erk and Akt compared to the control group. The activation of Erk and Akt by TCS-401 is blocked by pretreatment with PD98059 and LY294002, respectively. CS-401 treatment activates the MEK/Erk and PI3K/Akt signaling pathways and induces proliferation, differentiation, and migration in RPE cells[1]. CS-401 dose dependently inhibits the RPTC-Sup-induced reduction of fibronectin and α-SMA. At 1 μM, TCS-401 reverses the levels of fibronectin and α-SMA about onefold and at a dose of 2 μM, TCS-401 brings back fibronectin and α-SMA expression to near normal levels[2].

Preparing Stock Solutions
Concentration Volume (DMSO) Mass 1 mg 5 mg 10 mg
1 mM 3.2603 mL 16.3015 mL 32.6030 mL
5 mM 0.6521 mL 3.2603 mL 6.5206 mL
10 mM 0.3260 mL 1.6302 mL 3.2603 mL
Cell Assay

Cell proliferation are examined using MTT assay. The 5×103 cells grown in a 96-well plate for 24 h are partially starved in DMEM/F12 supplemented with 1% FBS for 12 h, and then stimulated with various concentrations of TCS-401 for an additional 24 h. MTT are added to the culture medium, and the cells are incubated for an additional 4 h. The formazan crystals formed are then dissolved by adding dimethyl sulfoxide (100 μL per well). Absorbance at 490 nm are measured using a microplate reader. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight






Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO: 11 mg/mL

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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