1. Metabolic Enzyme/Protease
  2. Phosphatase
  3. TCS 401

TCS 401 

Cat. No.: HY-12312 Purity: ≥98.0%
COA Handling Instructions

TCS 401 is a selective inhibitor of protein tyrosine phosphatase 1B (PTP1B).

For research use only. We do not sell to patients.

TCS 401 Chemical Structure

TCS 401 Chemical Structure

CAS No. : 243966-09-8

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50 mg USD 660 In-stock
100 mg USD 1080 In-stock
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Customer Review

Based on 3 publication(s) in Google Scholar

Top Publications Citing Use of Products

    TCS 401 purchased from MedChemExpress. Usage Cited in: J Tissue Eng Regen Med. 2020 Apr;14(4):575-587.  [Abstract]

    The activation of Src by treatment with the 152RM peptide is reversed by pretreatment with the PTP1B inhibitor TCS-401, and the increased phosphorylation of β-catenin Y654 by treatment with the 152RM peptide is reduced by pretreatment with the Src inhibitor bosutinib.
    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    TCS 401 is a selective inhibitor of protein tyrosine phosphatase 1B (PTP1B).

    In Vitro

    TCS-401 (0.5, 1, 2 μM) significantly increases the proliferation of RPE cells. TCS-401 significantly increases the expression of cyclin A and cyclin D1 at the concentrations of 1 and 2 μM in a concentration-dependent manner. TCS-401 at concentrations of 0.5, 1, and 2 μM significantly increases phosphorylation of Erk and Akt compared to the control group. The activation of Erk and Akt by TCS-401 is blocked by pretreatment with PD98059 and LY294002, respectively. CS-401 treatment activates the MEK/Erk and PI3K/Akt signaling pathways and induces proliferation, differentiation, and migration in RPE cells[1]. CS-401 dose dependently inhibits the RPTC-Sup-induced reduction of fibronectin and α-SMA. At 1 μM, TCS-401 reverses the levels of fibronectin and α-SMA about onefold and at a dose of 2 μM, TCS-401 brings back fibronectin and α-SMA expression to near normal levels[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    306.72

    Formula

    C10H11ClN2O5S

    CAS No.
    Appearance

    Solid

    Color

    Light yellow to khaki

    SMILES

    O=C(C1=C(NC(C(O)=O)=O)SC2=C1CCNC2)O.[H]Cl

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    0.1 M NaOH : 6.67 mg/mL (21.75 mM; ultrasonic and adjust pH to 9 with NaOH)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.2603 mL 16.3015 mL 32.6030 mL
    5 mM 0.6521 mL 3.2603 mL 6.5206 mL
    10 mM 0.3260 mL 1.6302 mL 3.2603 mL
    *Please refer to the solubility information to select the appropriate solvent.
    Purity & Documentation
    References
    Cell Assay
    [1]

    Cell proliferation are examined using MTT assay. The 5×103 cells grown in a 96-well plate for 24 h are partially starved in DMEM/F12 supplemented with 1% FBS for 12 h, and then stimulated with various concentrations of TCS-401 for an additional 24 h. MTT are added to the culture medium, and the cells are incubated for an additional 4 h. The formazan crystals formed are then dissolved by adding dimethyl sulfoxide (100 μL per well). Absorbance at 490 nm are measured using a microplate reader.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
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    TCS 401 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    TCS 401
    Cat. No.:
    HY-12312
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