Timosaponin BII (Synonyms: Prototimosaponin A III)

Name: Timosaponin BII
Brand: MedChemExpress
SKU: HY-N0812
Rating: 4.5 (1 reviews)

Cat. No.: HY-N0812 Purity: 99.45%: FAQs
Data Sheet SDS COA Handling Instructions

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Timosaponin BII (Prototimosaponin A III) is a steroid saponin found in the rhizomes of Anemarrhena asphodeloides. Timosaponin BII has neuronal protective, anti-inflammatory and antioxidant activities.

For research use only. We do not sell to patients.

Timosaponin BII Chemical Structure

CAS No. : 136656-07-0

Size	Price	Stock	Quantity
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 162	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 162	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 38	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
10 mg		Get quote
50 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Description

In Vitro

Timosaponin BII is a steroidal glycoside separated from Zhi Mu, is found to have the inhibitory activity against the proliferation of HL-60 (leukemic), Hela (cervix), HepG2 and Bel-7402 (liver), HT-29 (colon), and MDA-MB-468 (breast) human carcinoma cell lines with an IC₅₀ value of 15.5 μg/mL in the HL-60 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Rat retinas in model group and vehicle control group manifest an apparent up-regulation of BACE1 expression. Meanwhile, the level of malonaldehyde (MDA), Aβ1-40 and β-CTF are increased. However, when comparing with the vehicle control group, the retinas in Timosaponin-BII treated group showed significantly less BACE1 and accumulated less Aβ1-40 or β-CTF. It also showed significantly decreased level of MDA and prolonged partial thromboplastin time^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

921.07

Formula

C₄₅H₇₆O₁₉

CAS No.

136656-07-0

Appearance

Solid

Color

White to off-white

SMILES

C[C@@]1([C@]([C@@H]2C)([H])[C@](O[C@]2(O)CC[C@H](C)CO[C@@H]([C@@H]([C@@H](O)[C@@H]3O)O)O[C@@H]3CO)([H])C4)[C@]4([H])[C@@](CC[C@@]5([H])[C@@]6(CC[C@H](O[C@@](O[C@H](CO)[C@H](O)[C@@H]7O)([H])[C@@H]7O[C@]([C@@H]([C@@H](O)[C@@H]8O)O)([H])O[C@@H]8CO)C5)C)([H])[C@]6([H])CC1

Structure Classification

Steroids

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (108.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (108.57 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.0857 mL	5.4285 mL	10.8569 mL
5 mM	0.2171 mL	1.0857 mL	2.1714 mL
10 mM	0.1086 mL	0.5428 mL	1.0857 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.71 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (2.71 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (2.71 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 50 mg/mL (54.28 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.45%

Select Batch:

Data Sheet (274 KB) SDS (0 KB)

COA (251 KB) RP-HPLC (216 KB)

Handling Instructions (2659 KB)

References

[1]. Guo J, et al. Cytotoxic activities of chemical constituents from rhizomes of Anemarrhena asphodeloides and their analogues. Arch Pharm Res. 2015;38(5):598-603. [Content Brief]

[2]. Huang JF, et al. Timosaponin-BII inhibits the up-regulation of BACE1 induced by ferric chloride in rat retina. BMC Complement Altern Med. 2012 Oct 22;12:189. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	1.0857 mL	5.4285 mL	10.8569 mL	27.1423 mL
	5 mM	0.2171 mL	1.0857 mL	2.1714 mL	5.4285 mL
	10 mM	0.1086 mL	0.5428 mL	1.0857 mL	2.7142 mL
	15 mM	0.0724 mL	0.3619 mL	0.7238 mL	1.8095 mL
	20 mM	0.0543 mL	0.2714 mL	0.5428 mL	1.3571 mL
	25 mM	0.0434 mL	0.2171 mL	0.4343 mL	1.0857 mL
	30 mM	0.0362 mL	0.1809 mL	0.3619 mL	0.9047 mL
	40 mM	0.0271 mL	0.1357 mL	0.2714 mL	0.6786 mL
	50 mM	0.0217 mL	0.1086 mL	0.2171 mL	0.5428 mL
	60 mM	0.0181 mL	0.0905 mL	0.1809 mL	0.4524 mL
	80 mM	0.0136 mL	0.0679 mL	0.1357 mL	0.3393 mL
	100 mM	0.0109 mL	0.0543 mL	0.1086 mL	0.2714 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Timosaponin BII Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Timosaponin BII136656-07-0Prototimosaponin A IIIAmyloid-ββ-amyloid peptideAβAbetaoxidativestressdementia-palliativeAlzheimer'sdiseaseADInhibitorinhibitorinhibit

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Timosaponin BII (Synonyms: Prototimosaponin A III)

Customer Review

Timosaponin BII Related Antibodies

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Biological Activity

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References

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Timosaponin BII Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

Timosaponin BII Related Classifications

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