2. PI3K/Akt/mTOR Autophagy
  3. PI3K Autophagy
  4. Vps34-PIK-III

Vps34-PIK-III 

Cat. No.: HY-12794 Purity: 99.02%
COA Handling Instructions

Vps34-PIK-III is an orally active and selective VPS34 inhibitor (IC50=18 nM). Vps34-PIK-III effectively inhibits autophagy and can be used as a molecular tool. vps34-PIK-III is also a PI3K inhibitor that inhibits the expression of genes in liver cancer stem cells (CSCs).

For research use only. We do not sell to patients.

Vps34-PIK-III Chemical Structure

Vps34-PIK-III Chemical Structure

CAS No. : 1383716-40-2

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Solution
10 mM * 1 mL in DMSO USD 163 In-stock
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10 mM * 1 mL
ready for reconstitution
 USD 163 In-stock
Solid
2 mg USD 92 In-stock
5 mg USD 172 In-stock
10 mg USD 277 In-stock
25 mg USD 620 In-stock
50 mg USD 1109 In-stock
100 mg USD 1980 In-stock
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Customer Review

Based on 4 publication(s) in Google Scholar

Vps34-PIK-III Related Antibodies

Top Publications Citing Use of Products

View All PI3K Isoform Specific Products:

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PI3Kα PI3Kβ PI3Kγ PI3Kδ PI3KC2α PI3KC2β PI3KC2γ Vps34 PI3K PI3KC3 p120γ

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Vps34-PIK-III is an orally active and selective VPS34 inhibitor (IC50=18 nM). Vps34-PIK-III effectively inhibits autophagy and can be used as a molecular tool. vps34-PIK-III is also a PI3K inhibitor that inhibits the expression of genes in liver cancer stem cells (CSCs)[1][2][3].

IC50 & Target[2]

Vps34

18 nM (IC50)

PI(3)Kδ

1.2 μM (IC50)

PI(3)Kγ

3.04 μM (IC50)

PI(3)Kα

3.96 μM (IC50)

In Vitro

Vps34-PIK-III (1, 5, 10 μM; 24 h) inhibits autophagy and LC3 lipidation in DLD1 cells[1].
Vps34-PIK-III (1, 5, 10 μM; 24 h) leads to an increase in the lipidated and nonlipidated forms of LC3 in DLD1 cells[1].
Vps34-PIK-III (5 μM; 24 h) significantly decreases the expression of stemness genes in HCC cells[2].
Vps34-PIK-III (5 μM; 24 h) suppresses liver CSCs via AMPK activation in Huh7 and MHCC97H cells[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Vps34-PIK-III Related Antibodies

Cell Viability Assay[1]

Cell Line: DLD1 cells
Concentration: 1, 5, 10 µM
Incubation Time: 24 h
Result: Prevented the degradation of autophagy substrates p62, NCOA4, NBR1, NDP52, and FTH1.

RT-PCR[1]

Cell Line: HCC cells
Concentration: 5 µM
Incubation Time: 24 h
Result: Inhibited stemness genes expression.

Western Blot Analysis[1]

Cell Line: Huh7 and MHCC97H cells
Concentration: 5 µM
Incubation Time: 24 h
Result: Inhibited liver CSCs by activating AMPK.
In Vivo

Vps34-PIK-III (10 mg/kg; p.o.; single) rapidly absorbed and shows moderate mean systemic clearance (30 mL/min/kg, approximately 33% of hepatic blood flow), with good oral bioavailability (F% = 47)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 mice[1].
Dosage: 10 mg/kg; 2 mg/kg
Administration: Oral administration; intravenous injection; single
Result: 1.19 Pharmacokinetic Parameters of Vps34-PIK-III in C57BL/6 mice[1].
IV (2 mg/kg) PO (10 mg/kg)
Tmax (h) 0.7
Cmax (nM) 2994
AUCinf (nM•h) 2855 6725
t1/2 (h) 1.2
CL (mL/min/kg) 30
Vdss (L/kg) 1.5
F (%) 47%
Molecular Weight

319.36

Appearance

Solid

Formula

C17H17N7
CAS No.
SMILES

NC1=NC=C(C2=CC=NC(NC3=CC=NC=C3)=N2)C(CC4CC4)=N1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : ≥ 31 mg/mL (97.07 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.1313 mL 15.6563 mL 31.3126 mL
5 mM 0.6263 mL 3.1313 mL 6.2625 mL
10 mM 0.3131 mL 1.5656 mL 3.1313 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (7.83 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (7.83 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (7.83 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation
References
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Vps34-PIK-III Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Keywords:

Vps34-PIK-III1383716-40-2PI3KAutophagyPhosphoinositide 3-kinaseCSCsHCCDLD1 cellscancerautophagyHuh7 cellMHCC97H cellInhibitorinhibitorinhibit

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