2. GPCR/G Protein Apoptosis
  3. LPL Receptor Apoptosis
  4. W146

W146 is a selective antagonist of sphingosine-1-phosphate receptor 1 (S1PR1) with an EC50 value of 398 nM.

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W146

W146 構造式

CAS 番号 : 909725-61-7

容量 価格（税別） 在庫状況 数量
1 mg $140 在庫あり
5 mg $550 在庫あり
10 mg $950 在庫あり
25 mg $1900 在庫あり
50 mg $3040 在庫あり
100 mg $4850 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

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Based on 5 publication(s) in Google Scholar

Other Forms of W146:

W146 関連抗体

Top Publications Citing Use of Products

LPL Receptor アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
LPAR1 LPAR2 LPAR3 LPAR5 S1PR1 S1PR2 S1PR3 S1PR4 S1PR5

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製品説明

W146 is a selective antagonist of sphingosine-1-phosphate receptor 1 (S1PR1) with an EC50 value of 398 nM.

IC50 & Target

EC50: 398 nM (S1PR1)[1].
体外実験

W146 is a S1PR1 antagonist with a Ki of ~70-80 nM[1].
W146 pretreatment significantly increases activated cleaved caspase-3 levels. The reduced EPCs apoptosis which induced by S1P is completely abolished after treatment with W146[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

W146 関連抗体

Apoptosis Analysis[2]

Cell Line: Endothelial progenitor cells (EPCs).
Concentration: 10 μM.
Incubation Time: 30 min before the addition of S1P.
Result: Increases activated cleaved caspase-3 levels.
体内実験

W146 (5 mg/kg, ip, prior to AMD3100 administration) pre-treatment shows approximately 8-fold increase of KSL-HSPC mobilization, measured by the CFU-G/M colony forming assays, compared to that in mice treated with AMD3100 alone[3]
The W146-mediated augmentation of KSL-HSPC mobilization is specific, because pretreatment of mice with W140 is unable to produce any effect on AMD3100-stimulated KSL-HSPC mobilization. Injections of W146, W140, JTE013, or Cay10444 do not alter the basal WBC count in mice[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Mice (4-6-week-old)[3]
.
Dosage: 5 mg/kg.
Administration: IP, 1 hour prior to AMD3100 (ADM) administration.
Result: Significantly increased in KSL-HSPC mobilization compared to that in mice pretreated with dextran followed by AMD3100 administration.
分子量

342.37

分子式

C16H27N2O4P
CAS 番号
Appearance

Solid

Color

White to off-white

SMILES

O=C(NC1=CC=CC(CCCCCC)=C1)[C@H](N)CCP(O)(O)=O
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : 22.22 mg/mL (64.90 mM; ultrasonic and adjust pH to 3 with TFA; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9208 mL 14.6041 mL 29.2082 mL
5 mM 0.5842 mL 2.9208 mL 5.8416 mL
10 mM 0.2921 mL 1.4604 mL 2.9208 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

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体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.22 mg/mL (6.48 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.22 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (22.2 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.22 mg/mL (6.48 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.22 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (22.2 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 99.83%

COA (243 KB) HNMR (236 KB) RP-HPLC (259 KB) MS (72 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.9208 mL 14.6041 mL 29.2082 mL 73.0204 mL
5 mM 0.5842 mL 2.9208 mL 5.8416 mL 14.6041 mL
10 mM 0.2921 mL 1.4604 mL 2.9208 mL 7.3020 mL
15 mM 0.1947 mL 0.9736 mL 1.9472 mL 4.8680 mL
20 mM 0.1460 mL 0.7302 mL 1.4604 mL 3.6510 mL
25 mM 0.1168 mL 0.5842 mL 1.1683 mL 2.9208 mL
30 mM 0.0974 mL 0.4868 mL 0.9736 mL 2.4340 mL
40 mM 0.0730 mL 0.3651 mL 0.7302 mL 1.8255 mL
50 mM 0.0584 mL 0.2921 mL 0.5842 mL 1.4604 mL
60 mM 0.0487 mL 0.2434 mL 0.4868 mL 1.2170 mL
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W146 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

W146909725-61-7W 146W-146LPL ReceptorApoptosisLysophospholipid ReceptorInhibitorinhibitorinhibit

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製品名:
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製品番号:
HY-101395
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