1. Stem Cell/Wnt
  2. Porcupine
    Wnt
  3. Wnt-C59

Wnt-C59 (Synonyms: C59)

製品番号: HY-15659 純度: 99.56%
取扱説明書

Wnt-C59 (C59) is a highly potent and oral porcupine (PORCN) inhibitor with an IC50 of 74 pM.

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Wnt-C59 構造式

Wnt-C59 構造式

CAS 番号 : 1243243-89-1

容量 価格(税別) 在庫状況 数量
10 mM * 1 mL in DMSO USD 61 在庫あり
Estimated Time of Arrival: December 31
5 mg USD 55 在庫あり
Estimated Time of Arrival: December 31
10 mg USD 88 在庫あり
Estimated Time of Arrival: December 31
25 mg USD 158 在庫あり
Estimated Time of Arrival: December 31
50 mg USD 255 在庫あり
Estimated Time of Arrival: December 31
100 mg USD 439 在庫あり
Estimated Time of Arrival: December 31
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

カスタマーレビュー

Based on 9 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Wnt-C59 purchased from MCE. Usage Cited in: Cell Physiol Biochem. 2017;44(3):1251-1262.

    The protein levels of Wnt3a and β-catenin are examined in MCF-7 spheres and MDA-MB-231 spheres with Wnt-C59 treatment or not via western blot analysis.
    • 生物活性

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    製品説明

    Wnt-C59 (C59) is a highly potent and oral porcupine (PORCN) inhibitor with an IC50 of 74 pM.

    IC50 & Target

    IC50: 74 pM (PORCN)[1]

    体外実験

    Wnt-C59 (C59) inhibits PORCN activity in vitro at nanomolar concentrations, as assessed by inhibition of Wnt palmitoylation, Wnt interaction with the carrier protein Wntless/WLS, Wnt secretion, and Wnt activation of β-catenin reporter activity. Wnt-C59 inhibits WNT3A-mediated activation of a multimerized TCF-binding site driving luciferase with an IC50 of 74 pM[1].

    体内実験

    Wnt-C59 displays good bioavailability in mice. Wnt-C59 blocks progression of mammary tumors in MMTV-WNT1 transgenic mice while downregulating Wnt/β-catenin target genes[1]. Wnt-C59 has the potential to eradicate cancer stem cells in human tumors. Wnt-C59 inhibits stemness properties of NPC cells in a dosage-dependent manner by arresting sphere formation in both HNE1 and SUNE1 cells[2].

    分子量

    379.45

    分子式

    C₂₅H₂₁N₃O

    CAS 番号

    1243243-89-1

    SMILES

    O=C(NC1=CC=C(C2=CC=CN=C2)C=C1)CC3=CC=C(C4=CC(C)=NC=C4)C=C3

    輸送条件

    Room temperature in continental US; may vary elsewhere.

    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : ≥ 73.3 mg/mL (193.17 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.6354 mL 13.1770 mL 26.3539 mL
    5 mM 0.5271 mL 2.6354 mL 5.2708 mL
    10 mM 0.2635 mL 1.3177 mL 2.6354 mL
    *Please refer to the solubility information to select the appropriate solvent.
    体内:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (6.59 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (6.59 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (6.59 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    参考文献
    細胞実験
    [2]

    1×104 HK1, CNE1, HNE1 and SUNE1 cells are seeded in 24-well plates, and Wnt-C59 (5 μM, 10 μM, and 20 μM) is added the next day. Cell confluence is determined by microscopy at 24, 48, 72, and 96 hours after seeding of cells. The IC50 is determined by MTT assay[2].

    MCE はこれらの方法の精度を確認していません。 こちらは参照専用です。

    動物実験
    [1]

    Mice[1]

    Female nude mice orthotopically transplanted with independent MMTV-WNT1 tumors are treated with vehicle or Wnt-C59 10 mg/kg once daily for 17 days. Tumor volumes are measured on alternate days[1].

    MCE はこれらの方法の精度を確認していません。 こちらは参照専用です。

    参考文献
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    • 希釈カルキュレーター

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2

    Keywords:

    Wnt-C59C59C 59C-59PorcupineWntInhibitorinhibitorinhibit

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    製品名:
    Wnt-C59
    製品番号:
    HY-15659
    数量:
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