(+)-Cloprostenol
Based on 1 Customer Validation
(+)-Cloprostenol is a prostaglandin F2α (PGF2α) analogue, and shows selective agonistic activity at the prostaglandin receptor.
For research use only. We do not sell to patients.
- Purity: 98.06%
- CAS No.: 54276-21-0
- Formula: C22H29ClO6
- Molecular Weight:424.92
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Storage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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PGF2α |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CHO | IC50 |
>10 μM
Compound: 3
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Affinity for Prostaglandin I2 receptor expressed in CHO cells
Affinity for Prostaglandin I2 receptor expressed in CHO cells
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[PMID: 10715159] |
| CHO | IC50 |
3700 nM
Compound: 3
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Affinity for Prostanoid EP2 receptor expressed in CHO cells
Affinity for Prostanoid EP2 receptor expressed in CHO cells
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[PMID: 10715159] |
| CHO | IC50 |
635 nM
Compound: 3
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Affinity for Prostanoid EP3 receptor expressed in CHO cell line
Affinity for Prostanoid EP3 receptor expressed in CHO cell line
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[PMID: 10715159] |
| COS-7 | IC50 |
2 nM
Compound: 3
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Affinity for human Prostanoid FP receptor expressed in COS-7 cells
Affinity for human Prostanoid FP receptor expressed in COS-7 cells
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[PMID: 10715159] |
| COS-7 | IC50 |
496 nM
Compound: 3
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Affinity for Prostanoid EP1 receptor expressed in COS-7 cells.
Affinity for Prostanoid EP1 receptor expressed in COS-7 cells.
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[PMID: 10715159] |
| HEK293 | IC50 |
>10 μM
Compound: 3
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Affinity for human Prostaglandin D2 receptor expressed in HEK293 cells
Affinity for human Prostaglandin D2 receptor expressed in HEK293 cells
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[PMID: 10715159] |
D-Cloprostenol and PGF2 alpha are equipotent, about 150 times more potent than dl-cloprostenol (P < 0.05) and approximately 280 times more potent than PGE1 in inhibiting [3H]PGF2 alpha binding to corpus luteum cell membranes. However, d-cloprostenol and PGF2 alpha are about 10 times more potent than dl-cloprostenol and approximately 95 times more potent than PGE1 in myometrial cell membranes[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 54276-21-0
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Appearance Oil
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Molecular Weight 424.92
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Formula C22H29ClO6
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Color Colorless to light yellow
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SMILES
OC(CCC/C=C\C[C@@H]1[C@H]([C@H](O)C[C@@H]1O)/C=C/[C@@H](O)COC2=CC(Cl)=CC=C2)=O
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Synonyms
D-Cloprostenol
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (235.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Ethanol : 50 mg/mL (117.67 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 5 mg/mL (11.77 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (11.77 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.88 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.88 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.88 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (270 KB)
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SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
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Handling Instructions (2659 KB)
References
[1]. Manca R, et al. Intra-vesicle administration of D-cloprostenol for induction of abortion in mid-gestation bitches. Anim Reprod Sci. 2008 Jun;106(1-2):133-42. Epub 2007 Apr 21. [Content Brief]
[2]. Re G, et al. Specific binding of dl-cloprostenol and d-cloprostenol to PGF2 alpha receptors in bovine corpus luteum and myometrial cell membranes. J Vet Pharmacol Ther. 1994 Dec;17(6):455-8. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Ethanol / DMSO | 1 mM | 2.3534 mL | 11.7669 mL | 23.5338 mL | 58.8346 mL |
| 5 mM | 0.4707 mL | 2.3534 mL | 4.7068 mL | 11.7669 mL | |
| 10 mM | 0.2353 mL | 1.1767 mL | 2.3534 mL | 5.8835 mL | |
| 15 mM | 0.1569 mL | 0.7845 mL | 1.5689 mL | 3.9223 mL | |
| 20 mM | 0.1177 mL | 0.5883 mL | 1.1767 mL | 2.9417 mL | |
| 25 mM | 0.0941 mL | 0.4707 mL | 0.9414 mL | 2.3534 mL | |
| 30 mM | 0.0784 mL | 0.3922 mL | 0.7845 mL | 1.9612 mL | |
| 40 mM | 0.0588 mL | 0.2942 mL | 0.5883 mL | 1.4709 mL | |
| 50 mM | 0.0471 mL | 0.2353 mL | 0.4707 mL | 1.1767 mL | |
| 60 mM | 0.0392 mL | 0.1961 mL | 0.3922 mL | 0.9806 mL | |
| 80 mM | 0.0294 mL | 0.1471 mL | 0.2942 mL | 0.7354 mL | |
| 100 mM | 0.0235 mL | 0.1177 mL | 0.2353 mL | 0.5883 mL |
(+)-Cloprostenol Related Classifications
HY-107381 Related Classifications
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