A12B4C3
Based on 1 Customer Validation
A12B4C3 is a potent human polynucleotide kinase/phosphatase (hPNKP) inhibitor with an IC50 value of 0.06 μM. A12B4C3 has antiproliferative activity against cancer cells. A12B4C3 can also enhance the radiosensitivity of certain cancer cells.
For research use only. We do not sell to patients.
- Purity: 98.06%
- CAS No.: 1005129-80-5
- Formula: C30H38N4O5
- Molecular Weight:534.65
-
Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
IC50: 0.06 μM (hPNKP)[1]
A12B4C3 (0-10 μM) strongly inhibits hPNKP phosphatase activity[1].
A12B4C3 (0-100 μM; 72 h) reduces A549 and MDA-MB-231 cell proliferation in a dose dependent manner[1].
A12B4C3 (1 μM; 24 h) increases the radiosensitivity of A549 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:A549 and MDA-MB-231
-
Concentration:0, 1, 5, 10, 50 and 100 μM
-
Incubation Time:72 h
-
Result:Reduced cell proliferation in a dose dependent manner, and up to ~50% at 100 μM.
-
Cell Line:A549 and MDA-MB-231 (incubated with A12B4C3 for 2h then irradiated 0-10 Gy)
-
Concentration:1 μM
-
Incubation Time:24 h
-
Result:Almost doubled the radiosensitivity of A549 cells.
Chemical Information
-
CAS No. 1005129-80-5
-
Appearance Solid
-
Molecular Weight 534.65
-
Formula C30H38N4O5
-
Color Light yellow to yellow
-
SMILES
O=C1N(C(C2N(C(C(CCCCCCCCCC)O)C=CC12)NC3=CC=C([N+]([O-])=O)C=C3)=O)C4=CC=CC=C4
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 53 mg/mL (99.13 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (271 KB)
-
SDS (479 KB)
- English - EN (479 KB)
- Français - FR (479 KB)
- Deutsch - DE (479 KB)
- Norwegian - NO (479 KB)
- Español - ES (479 KB)
- Swedish - SV (479 KB)
- Italian - IT (479 KB)
- Portuguese - PT (479 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8704 mL | 9.3519 mL | 18.7038 mL | 46.7596 mL |
| 5 mM | 0.3741 mL | 1.8704 mL | 3.7408 mL | 9.3519 mL | |
| 10 mM | 0.1870 mL | 0.9352 mL | 1.8704 mL | 4.6760 mL | |
| 15 mM | 0.1247 mL | 0.6235 mL | 1.2469 mL | 3.1173 mL | |
| 20 mM | 0.0935 mL | 0.4676 mL | 0.9352 mL | 2.3380 mL | |
| 25 mM | 0.0748 mL | 0.3741 mL | 0.7482 mL | 1.8704 mL | |
| 30 mM | 0.0623 mL | 0.3117 mL | 0.6235 mL | 1.5587 mL | |
| 40 mM | 0.0468 mL | 0.2338 mL | 0.4676 mL | 1.1690 mL | |
| 50 mM | 0.0374 mL | 0.1870 mL | 0.3741 mL | 0.9352 mL | |
| 60 mM | 0.0312 mL | 0.1559 mL | 0.3117 mL | 0.7793 mL | |
| 80 mM | 0.0234 mL | 0.1169 mL | 0.2338 mL | 0.5845 mL |
Powered by Bioz