Ac-RFAAKAA-COOH
Based on 1 Customer Validation
Ac-RFAAKAA-COOH is a cysteine-containing polypeptide with a reactive lysine residue, and serves as a substrate for covalent modification and chemical reactivity assays. Ac-RFAAKAA-COOH forms stable covalent adducts with isothiocyanates, resulting in peptide depletion over time. Ac-RFAAKAA-COOH acts as a model peptide in direct peptide reactivity assays, where its chemical reactivity is evaluated via fluorescamine-based free amine assays. Ac-RFAAKAA-COOH can be used in studies related to allergic contact dermatitis and skin sensitization.
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- Reinheit: 99.54%
- CAS. Nr.: 845727-63-1
- Formel: C35H57N11O9
- Molecular Weight:775.90
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Speicherung:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Biologische Aktivität
When used in a spectrophotometric depletion assay with a 30% depletion threshold (and a 10% depletion threshold for cysteine peptides), Ac-RFAAKAA-COOH can accurately predict the skin sensitization potential of test chemicals, with a sensitivity of 80.0%, specificity of 86.7%, and accuracy of 82.5%[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 845727-63-1
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Appearance Solid
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Molecular Weight 775.90
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Formel C35H57N11O9
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Color White to off-white
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Sequence
Ac-Arg-Phe-Ala-Ala-Lys-Ala-Ala
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Sequence Shortening
Ac-RFAAKAA
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Lösungsmittel & Löslichkeit
H2O : 50 mg/mL (64.44 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Reinheit & Dokumentation
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Data Sheet (271 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Karlsson I, et al. Peptide Reactivity of Isothiocyanates--Implications for Skin Allergy. Sci Rep. 2016;6:21203. Published 2016 Feb 17. [Content Brief]
[2]. Cho SA, et al. Method for detecting the reactivity of chemicals towards peptides as an alternative test method for assessing skin sensitization potential. Toxicol Lett. 2014;225(1):185-191. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.2888 mL | 6.4441 mL | 12.8883 mL | 32.2206 mL |
| 5 mM | 0.2578 mL | 1.2888 mL | 2.5777 mL | 6.4441 mL | |
| 10 mM | 0.1289 mL | 0.6444 mL | 1.2888 mL | 3.2221 mL | |
| 15 mM | 0.0859 mL | 0.4296 mL | 0.8592 mL | 2.1480 mL | |
| 20 mM | 0.0644 mL | 0.3222 mL | 0.6444 mL | 1.6110 mL | |
| 25 mM | 0.0516 mL | 0.2578 mL | 0.5155 mL | 1.2888 mL | |
| 30 mM | 0.0430 mL | 0.2148 mL | 0.4296 mL | 1.0740 mL | |
| 40 mM | 0.0322 mL | 0.1611 mL | 0.3222 mL | 0.8055 mL | |
| 50 mM | 0.0258 mL | 0.1289 mL | 0.2578 mL | 0.6444 mL | |
| 60 mM | 0.0215 mL | 0.1074 mL | 0.2148 mL | 0.5370 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Ac-RFAAKAA-COOH Related Classifications
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