AChE-IN-9
AChE-IN-9 is a Tacrine (HY-111338) glycoconjugate tethered with acetylated β-Glucose. AChE-IN-9 is also an AChE inhibitor with an IC50 value of 0.4 μM, with lower hepatotoxicity on healthy cells. Tacrine is used in Alzheimer's research.
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- 화학식: C30H35N5O9
- 분자량:609.63
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보관:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocol
- 순도&문서
- References
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Help & FAQs
Biological Activity
제품 설명
IC50 & Target
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AChE 0.4 μM (IC50) |
In Vitro
Tacrine (HY-111338) is mainly metabolized by CYP 1A2, but its glycoconjugates have shown more affinity towards CYP 3A4 rather than CYP 1A2[1].
AChE-IN-9 (compound A-1) (200 μM; 24 h) is non-toxic on HePG2 cell line with 100% cell viability, and shows lower hepatotoxicity than Tacrine (HY-111338) on healthy HepG2 cells[1].
AChE-IN-9 (10 μM; 24 h) inhibits AChE activity with inhibition rate of 96.6%[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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분자량 609.63
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화학식 C30H35N5O9
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SMILES
CC(OC[C@H]1O[C@@H]([C@@H]([C@H]([C@@H]1OC(C)=O)OC(C)=O)OC(C)=O)N2C=C(N=N2)CNC3=C4CCCCC4=NC5=CC=CC=C53)=O
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocol
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Hepatotoxicity Study
This protocol evaluates hepatotoxicity using complementary in vivo mouse APAP acute liver injury and in vitro hepatocyte-based cytotoxicity readouts. In vivo APAP injury is assessed by serum ALT/AST, liver histology, hepatic glutathione, protein adducts, necrosis, inflammation, and regeneration-related endpoints. In vitro hepatotoxicity is assessed by loss of viability, leakage of ALT/AST/LDH, oxidative-stress markers, mitochondrial function, nuclear morphology, intracellular calcium, and high-content imaging endpoints.
순도&문서
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
AChE-IN-9 Related Classifications
HY-143291 Related Classifications
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