ADU-1805
ADU-1805 is a humanized monoclonal IgG2 antibody, a selective, pan-allelic anti-SIRPα antibody, with a Kd value of 11.0 × 10-9 M for hSIRPαV1. ADU-1805 binds to all known hSIRPα alleles. ADU-1805 blocks the SIRPα-CD47 interaction, promotes phagocytosis of cells, and enhances trogocytosis of cells. ADU-1805 can be used in research related to Burkitt lymphoma.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : ≥99.0%
- Molecular Weight:145 kDa
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Biologische Aktivität
Beschreibung
Isotype
Human IgG2 kappa
Recommend Isotype Controls
Species Reactivity
Human
In Vitro
ADU-1805 binds to hSIRPαV1 with a Kd value of 11.0 × 10-9 M, and exhibits binding properties similar to those of the parental and chimeric hSIRPα.40A antibody variants[1].
ADU-1805 potently binds to primary monocytes and neutrophils, while exhibits extremely weak binding to lymphocytes[1].
ADU-1805 (0.67-66.7 nM; 2 h) promotes Rituximab (HY-P9913)-mediated phagocytosis of Raji Burkitt's lymphoma cells by peripheral blood-derived macrophages in a concentration-dependent manner[1].
ADU-1805 (0.067-66.7 nM; 2 h) enhances Rituximab-mediated trogocytosis of primary neutrophils against BJAB Burkitt's lymphoma cells in a concentration-dependent manner[1].
ADU-1805 does not bind to primary red blood cells or platelets, nor does it induce hemagglutination of red blood cells[1].
ADU-1805 (5 days) neither inhibits T cell activation nor reduces T cell counts in allogeneic mixed lymphocyte reactions[1].
ADU-1805 (100 μg/mL; 3 days) does not inhibit SEB-induced T cell activation and proliferation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Macaca fascicularis (5-7 years old; male and female; purpose-bred from Asia)[1]
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Dosage:0.3 mg/kg; 3 mg/kg; 30 mg/kg
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Administration:i.v.; single 15-minute infusion
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Result:Showed dose-proportional exposure increase for 3 mg/kg and 30 mg/kg dose levels, with an estimated half-life of 1.86-6.41 days.
Demonstrated no test-article related changes in clinical observations, body weight, food consumption, neurologic or cardiovascular parameters, serum chemistry, hematology, coagulation, organ weights, or histopathology.
Maintained stable hemoglobin levels across all dose groups, with no signs of acute anemia or thrombocytopenia.
Gene ID
Accession
Target
SIRPa/CD172a
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Anwendung
ELISA, FACS, Functional assay
Verified Bioactivity
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Immobilized Human SIRP alpha Protein can bind ADU-1805. The ED50 for this effect is 192 ng/mL.
Chemical Information
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Appearance Liquid
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Molecular Weight 145 kDa
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Color Colorless to light yellow
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Versand
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Protokoll
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Phagocytosis Functional Assay
A phagocytosis functional assay measures the ability of phagocytic cells, such as neutrophils, macrophages, monocytes, or microglia/macrophages, to bind and internalize particulate targets including bacteria, yeast particles, beads, or myelin particles. Fluorescent flow-cytometry assays detect target uptake as fluorescence associated with gated phagocytes, while pH-sensitive dyes such as pHrodo increase signal in acidic phagosomal compartments and therefore preferentially report internalized particles rather than particles remaining outside the cell. Microscopy or high-content imaging can be used to confirm intracellular localization and, in some protocols, to follow uptake kinetics.
Reinheit & Dokumentation
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Data Sheet (261 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
ADU-1805 Related Classifications
HY-P992176 Related Classifications
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