Anemoside A3-methyl 6-aminohexanoate
Based on 1 Customer Validation
Anemoside A3-methyl 6-aminohexanoate is an orally effective pyruvate carboxylase (PC) inhibitor with a Kd value of 10.1 μM against human PC. Anemoside A3-methyl 6-aminohexanoate exerts its function through the PC/NF-κB/NLRP3 inflammasome axis. Anemoside A3-methyl 6-aminohexanoate significantly alleviates symptoms of DSS (HY-116282C)-induced colitis in mice. Anemoside A3-methyl 6-aminohexanoate can be used in colitis-related research.
For research use only. We do not sell to patients.
- Purity: 98.62%
- CAS No.: 2919975-48-5
- Formula: C48H79NO13
- Molecular Weight:878.14
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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NF-κB |
IL-6 |
IL-1β |
NLRP3 |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| THP-1 | IC50 |
>=80 μM
Compound: A3-6
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Cytotoxicity against human THP-1 cells assessed as inhibition of cell viability incubated for 24 hrs by CCK8 assay
Cytotoxicity against human THP-1 cells assessed as inhibition of cell viability incubated for 24 hrs by CCK8 assay
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[PMID: 38687956] |
Anemoside A3-methyl 6-aminohexanoate (Compound A3-6) (0-10 μM; 0-25 h) potently inhibits PC enzyme activity in THP-1-derived macrophages with an IC50 of 0.058 μM, reduces LPS (HY-D1056)-induced proinflammatory cytokine secretion, and regulates pyruvate metabolism in a concentration-dependent manner[1].
Anemoside A3-methyl 6-aminohexanoate (1-10 μM; 0-13.5 h) dose-dependently inhibits NF-κB pathway activation and NLRP3 inflammasome activation in THP-1-derived macrophages[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:THP-1-derived macrophages
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Concentration:1, 10 μM (NF-κB pathway measurement); 1, 10 μM (NLRP3 inflammasome measurement)
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Incubation Time:1 h prior to 6 h LPS stimulation (NF-κB pathway measurement); 1 h prior to 12 h LPS + 0.5 h ATP stimulation (NLRP3 inflammasome measurement)
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Result:Inhibited LPS-induced increases in p-p65 and p-IκBα protein levels in a concentration-dependent manner.
Significantly reduced LPS + ATP-induced NLRP3, Pro-IL-1β, P20, and IL-1β protein levels.
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Cell Line:Differentiated THP-1 cells treated LPS
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Concentration:0.1, 1, 10 μM
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Incubation Time:1 h prior to 24 h LPS stimulation
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Result:Significantly decreased TNF-α
and IL-6 secretion in a concentration-dependent manner.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:ICR mice (male, 20-25 g, DSS-induced ulcerative colitis)[1]
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Dosage:15 mg/kg; 30 mg/kg; 60 mg/kg
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Administration:i.g.; daily; 8 days
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Result:Reduced DSS-induced body weight loss.
Dose-dependently decreased elevated disease activity index (DAI) scores.
Dose-dependently prevented colon shortening.
Reduced splenomegaly (lowered spleen index) in a dose-dependent manner.
Reduced colonic levels of the pro-inflammatory cytokine IL-6 in a concentration-dependent manner.
Decreased colon tissue pyruvate carboxylase (PC) activity at 60 mg/kg.
Increased colonic pyruvate levels at 60 mg/kg.
Inhibited DSS-induced IκBα degradation and P65 phosphorylation in mouse colon tissues.
Chemical Information
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CAS No. 2919975-48-5
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Appearance Solid
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Molecular Weight 878.14
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Formula C48H79NO13
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Color White to off-white
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SMILES
C=C(C)[C@@H]1CC[C@]2(C(NCCCCCC(OC)=O)=O)CC[C@@]3(C)[C@]4(C)CC[C@@]5([H])[C@](C)(CO)[C@@H](O[C@@H]6OC[C@H](O)[C@H](O)[C@H]6O[C@@H]7O[C@@H](C)[C@H](O)[C@@H](O)[C@H]7O)CC[C@]5(C)[C@@]4([H])CC[C@]3([H])[C@]21[H]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
DMSO : ≥ 100 mg/mL (113.88 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (280 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.1388 mL | 5.6939 mL | 11.3877 mL | 28.4693 mL |
| 5 mM | 0.2278 mL | 1.1388 mL | 2.2775 mL | 5.6939 mL | |
| 10 mM | 0.1139 mL | 0.5694 mL | 1.1388 mL | 2.8469 mL | |
| 15 mM | 0.0759 mL | 0.3796 mL | 0.7592 mL | 1.8980 mL | |
| 20 mM | 0.0569 mL | 0.2847 mL | 0.5694 mL | 1.4235 mL | |
| 25 mM | 0.0456 mL | 0.2278 mL | 0.4555 mL | 1.1388 mL | |
| 30 mM | 0.0380 mL | 0.1898 mL | 0.3796 mL | 0.9490 mL | |
| 40 mM | 0.0285 mL | 0.1423 mL | 0.2847 mL | 0.7117 mL | |
| 50 mM | 0.0228 mL | 0.1139 mL | 0.2278 mL | 0.5694 mL | |
| 60 mM | 0.0190 mL | 0.0949 mL | 0.1898 mL | 0.4745 mL | |
| 80 mM | 0.0142 mL | 0.0712 mL | 0.1423 mL | 0.3559 mL | |
| 100 mM | 0.0114 mL | 0.0569 mL | 0.1139 mL | 0.2847 mL |
Anemoside A3-methyl 6-aminohexanoate Related Classifications
HY-161627 Related Classifications
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