ANO1-IN-4
Based on 1 Customer Validation
ANO1-IN-4 (Compound 10bm) is a reversible inhibitor for calcium-activated chloride channel transmembrane protein 16A (TMEM16A, also known as ANO1) with an IC50 of 0.030 µM. ANO1-IN-4 exhibits good metabolic stability in rat liver microsomes. ANO1-IN-4 inhibits spontaneous contraction in mouse isolated ileum.
For research use only. We do not sell to patients.
- Purity: 99.58%
- CAS No.: 2098490-06-1
- Formula: C19H19BrF2N2O2S
- Molecular Weight:457.33
-
Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
Membrane Transporter/Ion Channel Compound Library
HY-L011
-
Mechanoreceptors Compound Library
HY-L095
-
Membrane Protein-targeted Compound Library
HY-L149
-
Membrane Receptor-targeted Compound Library
HY-L150
-
Ion Channel Compound Library
HY-L166
-
Bioactive Compound Library Max
HY-L181
-
MCE Bioactive Compound Library
HY-L001V
-
Bioactive Compound Library
HY-L001
-
High-Throughput Bioactive Compound Library
HY-L205
Biological Activity
IC50: 0.030 µM (ANO1)
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| U138-MG | GI50 |
29.4 μM
Compound: 4
|
Antiproliferative activity against human U138 cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay
Antiproliferative activity against human U138 cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay
|
[PMID: 32906067] |
| U-251 | GI50 |
45.33 μM
Compound: 4
|
Antiproliferative activity against human U251 cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay
Antiproliferative activity against human U251 cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay
|
[PMID: 32906067] |
| U-87MG ATCC | GI50 |
48 μM
Compound: 4
|
Antiproliferative activity against human U87MG cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay
Antiproliferative activity against human U87MG cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay
|
[PMID: 32906067] |
Chemical Information
-
CAS No. 2098490-06-1
-
Appearance Solid
-
Molecular Weight 457.33
-
Formula C19H19BrF2N2O2S
-
Color White to off-white
-
SMILES
O=C(C1=C(NC(C(F)(Br)F)=O)SC2=C1CCCCC2)NC3=C(C)C=CC=C3
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 50 mg/mL (109.33 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (278 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1866 mL | 10.9330 mL | 21.8660 mL | 54.6651 mL |
| 5 mM | 0.4373 mL | 2.1866 mL | 4.3732 mL | 10.9330 mL | |
| 10 mM | 0.2187 mL | 1.0933 mL | 2.1866 mL | 5.4665 mL | |
| 15 mM | 0.1458 mL | 0.7289 mL | 1.4577 mL | 3.6443 mL | |
| 20 mM | 0.1093 mL | 0.5467 mL | 1.0933 mL | 2.7333 mL | |
| 25 mM | 0.0875 mL | 0.4373 mL | 0.8746 mL | 2.1866 mL | |
| 30 mM | 0.0729 mL | 0.3644 mL | 0.7289 mL | 1.8222 mL | |
| 40 mM | 0.0547 mL | 0.2733 mL | 0.5467 mL | 1.3666 mL | |
| 50 mM | 0.0437 mL | 0.2187 mL | 0.4373 mL | 1.0933 mL | |
| 60 mM | 0.0364 mL | 0.1822 mL | 0.3644 mL | 0.9111 mL | |
| 80 mM | 0.0273 mL | 0.1367 mL | 0.2733 mL | 0.6833 mL | |
| 100 mM | 0.0219 mL | 0.1093 mL | 0.2187 mL | 0.5467 mL |
ANO1-IN-4 Related Classifications
HY-153508 Related Classifications
Powered by Bioz