Anti-Human/Mouse Amphiregulin Antibody (AR37)
Based on 1 Customer Validation
Anti-Human/Mouse Amphiregulin Antibody (AR37) is an antibody targeting amphiregulin (AREG), with a Kd value of 3.24 nM for the human protein and 6.40 nM for the mouse protein. Anti-Human/Mouse Amphiregulin Antibody (AR37) blocks the autocrine activation of the AREG-EGFR-ERK axis. Anti-Human/Mouse Amphiregulin Antibody (AR37) inhibits the proliferation and migration of ovarian cancer cells. Anti-Human/Mouse Amphiregulin Antibody (AR37) prolongs the survival of immunocompetent mice bearing syngeneic ovarian cancer tumors. Anti-Human/Mouse Amphiregulin Antibody (AR37) is applicable to ovarian cancer-related research.
For research use only. We do not sell to patients.
- Purity : 99.544%
- Molecular Weight:142.82 kDa
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Purity & Documentation
- References
-
Help & FAQs
All EGFR Isoforms
More
Biological Activity
Description
Isotype
Mouse IgG1 lambda
Recommend Isotype Controls
Species Reactivity
Human/Mouse
IC50 & Target
Amphiregulin
In Vitro
Anti-Human/Mouse Amphiregulin Antibody (AR37) (100 ng/mL human amphiregulin; 1-7 μg/mL) dose-dependently blocks amphiregulin-induced EGFR and ERK activation in HeLa cells[1].
Anti-Human/Mouse Amphiregulin Antibody (AR37) dose-dependently inhibits DNA synthesis in ID8 murine ovarian cancer cells[1].
Anti-Human/Mouse Amphiregulin Antibody (AR37) (16 h) inhibits migration of ID8 murine ovarian cancer cells by blocking autocrine amphiregulin signaling[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Anti-Human/Mouse Amphiregulin Antibody (AR37) (0.2 mg/injection; i.p.; twice weekly; starting from day 10) significantly prolongs the survival of C57/Black mice bearing tp53-/- AR-/- AR OX ID8 ovarian tumors[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:C57/Black (female, 6 weeks old, injected intraperitoneally with 5×106 ID8 ovarian cancer cells)[1]
-
Dosage:0.2 mg/injection
-
Administration:i.p.; twice a week; day 10 to day 45
-
Result:Reduced abdominal fluid volume to near 0 mL.
Significantly prolonged animal survival.
-
Animal Model:C57/Black (female, 6 weeks old, injected intraperitoneally with 5×106 tp53-/- AR-/- AR OX ID8 ovarian cancer cells)[1]
-
Dosage:0.2 mg/injection
-
Administration:i.p.; twice a week; day 10 onward
-
Result:Significantly prolonged survival of mice bearing tp53-/- AR-/- AR OX ID8 tumors.
Gene ID
Accession
P15514
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Format
-
Product Image
Application
in vivo blocking of amphiregulin (AREG); in vitro blocking of amphiregulin (AREG); Immunofluorescenc
Chemical Information
-
Appearance Liquid
-
Molecular Weight 142.82 kDa
-
Color Colorless to light yellow
-
Formulation
Please refer to the lot-specific COA for specific buffer information.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
-
Data Sheet (261 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Inhibitory Antibodies User Guide (603 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Human/Mouse Amphiregulin Antibody (AR37) Related Classifications
HY-P991748 Related Classifications
Powered by Bioz