Anticancer agent 126
Based on 1 Customer Validation
Anticancer agent 126 (compound 12) is a WDR5 inhibitor with anticancer effects. Anticancer agent 126 disrupts WDR5-MYC interaction in cells and reduce MYC target gene expression.
For research use only. We do not sell to patients.
- Purity : 98.05%
- CAS No.: 3033748-20-5
- Formula: C25H18F3N3O4S
- Molecular Weight:513.49
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| COR-L279 | GI50 |
4.2 μM
Compound: 12
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Antiproliferative activity against human COR-L279 cells assessed as cell growth inhibition measured after 72 hrs by CellTier-Glo assay
Antiproliferative activity against human COR-L279 cells assessed as cell growth inhibition measured after 72 hrs by CellTier-Glo assay
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[PMID: 37307526] |
| Daudi | GI50 |
15.4 μM
Compound: 12
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Antiproliferative activity against human Daudi cells assessed as cell growth inhibition measured after 72 hrs by CellTier-Glo assay
Antiproliferative activity against human Daudi cells assessed as cell growth inhibition measured after 72 hrs by CellTier-Glo assay
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[PMID: 37307526] |
| HL-60 | GI50 |
4.6 μM
Compound: 12
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Antiproliferative activity against human HL-60 cells assessed as cell growth inhibition measured after 72 hrs by CellTier-Glo assay
Antiproliferative activity against human HL-60 cells assessed as cell growth inhibition measured after 72 hrs by CellTier-Glo assay
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[PMID: 37307526] |
| NB-4 | GI50 |
7.1 μM
Compound: 12
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Antiproliferative activity against human NB4 cells assessed as cell growth inhibition measured after 72 hrs by CellTier-Glo assay
Antiproliferative activity against human NB4 cells assessed as cell growth inhibition measured after 72 hrs by CellTier-Glo assay
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[PMID: 37307526] |
| NCI-H1650 | GI50 |
22.6 μM
Compound: 12
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Antiproliferative activity against human NCI-H1650 cells assessed as cell growth inhibition measured after 72 hrs by CellTier-Glo assay
Antiproliferative activity against human NCI-H1650 cells assessed as cell growth inhibition measured after 72 hrs by CellTier-Glo assay
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[PMID: 37307526] |
| NCI-H520 | GI50 |
9.4 μM
Compound: 12
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Antiproliferative activity against human NCI-H520 cells assessed as cell growth inhibition measured after 72 hrs by CellTier-Glo assay
Antiproliferative activity against human NCI-H520 cells assessed as cell growth inhibition measured after 72 hrs by CellTier-Glo assay
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[PMID: 37307526] |
| Ramos | GI50 |
4.3 μM
Compound: 12
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Antiproliferative activity against human Ramos cells assessed as cell growth inhibition measured after 72 hrs by CellTier-Glo assay
Antiproliferative activity against human Ramos cells assessed as cell growth inhibition measured after 72 hrs by CellTier-Glo assay
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[PMID: 37307526] |
| SHP77 | GI50 |
16.6 μM
Compound: 12
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Antiproliferative activity against human SHP77 cells assessed as cell growth inhibition measured after 72 hrs by CellTier-Glo assay
Antiproliferative activity against human SHP77 cells assessed as cell growth inhibition measured after 72 hrs by CellTier-Glo assay
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[PMID: 37307526] |
Chemical Information
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CAS No. 3033748-20-5
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Appearance Solid
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Molecular Weight 513.49
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Formula C25H18F3N3O4S
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Color White to off-white
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SMILES
FC1=C(C(F)=CC(C2=CC(S(=O)(F)=O)=CC=C2)=C1)CNC(C3=NN(C(C)=CC3=O)C4=CC=CC=C4)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
In Vitro:
DMSO : 6.67 mg/mL (12.99 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocols
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RT-PCR
Reverse transcription technology uses RNA as a template to synthesize DNA. RT-PCR is simple, specific and sensitive, and can be used to detect gene expression levels and expression differences in cells; detect RNA virus content; clone cDNA sequences of specific genes.
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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Real Time qPCR (Q-PCR)
Real-time quantitative PCR (qPCR) quantifies an amplifiable nucleic-acid target by monitoring fluorescence during PCR cycling rather than measuring product only after amplification. The increase in fluorescence tracks accumulation of PCR product, and the quantification cycle (Cq; historically also Ct/CP) is related to the initial amount of target: samples containing more starting target generally reach the defined fluorescence threshold in fewer cycles.
Purity & Documentation
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Data Sheet (270 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9475 mL | 9.7373 mL | 19.4746 mL | 48.6864 mL |
| 5 mM | 0.3895 mL | 1.9475 mL | 3.8949 mL | 9.7373 mL | |
| 10 mM | 0.1947 mL | 0.9737 mL | 1.9475 mL | 4.8686 mL |
Anticancer agent 126 Related Classifications
HY-149873 Related Classifications
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