Antiviral agent 24
Based on 1 Customer Validation
Antiviral agent 24 is a potent antiviral agent with EC50 values of 0.101, 19.9, 91.2 µM for EV71, CVA21, EV68, respectively. Antiviral agent 24 inhibits METTL3/METTL14 activity in a dose-dependent manner.
For research use only. We do not sell to patients.
- Purity: 99.63%
- CAS No.: 23661-03-2
- Formula: C18H18F3N5O4
- Molecular Weight:425.36
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| G-361 | IC50 |
>166.7 μM
Compound: 44
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Antitumor activity against human G361 cells after 72 hrs by Calcein AM assay
Antitumor activity against human G361 cells after 72 hrs by Calcein AM assay
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[PMID: 17418578] |
| HOS | IC50 |
>166.7 μM
Compound: 44
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Antitumor activity against human HOS cells after 72 hrs by Calcein AM assay
Antitumor activity against human HOS cells after 72 hrs by Calcein AM assay
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[PMID: 17418578] |
| K562 | IC50 |
>166.7 μM
Compound: 44
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Antitumor activity against human K562 cells after 72 hrs by Calcein AM assay
Antitumor activity against human K562 cells after 72 hrs by Calcein AM assay
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[PMID: 17418578] |
| RD | CC50 |
>100 μM
Compound: 2; 11c
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Cytotoxicity against human RD cells by MTS assay
Cytotoxicity against human RD cells by MTS assay
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[PMID: 36455356] |
| RD | CC50 |
>235 μM
Compound: 8
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Cytotoxicity against human RD cells
Cytotoxicity against human RD cells
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[PMID: 38128296] |
| Vero | CC50 |
>50 μM
Compound: 8
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Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 7 days by resazurin staining based analysis
Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 7 days by resazurin staining based analysis
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[PMID: 38128296] |
| Vero C1008 | CC50 |
>100 μM
Compound: 2; 11c
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Cytotoxicity against african green monkey Vero E6 cells by MTS assay
Cytotoxicity against african green monkey Vero E6 cells by MTS assay
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[PMID: 36455356] |
Chemical Information
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CAS No. 23661-03-2
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Appearance Solid
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Molecular Weight 425.36
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Formula C18H18F3N5O4
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Color White to off-white
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SMILES
OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C(N=CN=C3NCC4=CC=CC(C(F)(F)F)=C4)=C3N=C2)O1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 250 mg/mL (587.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.89 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.89 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3509 mL | 11.7547 mL | 23.5095 mL | 58.7737 mL |
| 5 mM | 0.4702 mL | 2.3509 mL | 4.7019 mL | 11.7547 mL | |
| 10 mM | 0.2351 mL | 1.1755 mL | 2.3509 mL | 5.8774 mL | |
| 15 mM | 0.1567 mL | 0.7836 mL | 1.5673 mL | 3.9182 mL | |
| 20 mM | 0.1175 mL | 0.5877 mL | 1.1755 mL | 2.9387 mL | |
| 25 mM | 0.0940 mL | 0.4702 mL | 0.9404 mL | 2.3509 mL | |
| 30 mM | 0.0784 mL | 0.3918 mL | 0.7836 mL | 1.9591 mL | |
| 40 mM | 0.0588 mL | 0.2939 mL | 0.5877 mL | 1.4693 mL | |
| 50 mM | 0.0470 mL | 0.2351 mL | 0.4702 mL | 1.1755 mL | |
| 60 mM | 0.0392 mL | 0.1959 mL | 0.3918 mL | 0.9796 mL | |
| 80 mM | 0.0294 mL | 0.1469 mL | 0.2939 mL | 0.7347 mL | |
| 100 mM | 0.0235 mL | 0.1175 mL | 0.2351 mL | 0.5877 mL |
Antiviral agent 24 Related Classifications
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