AP232 dihydrochloride
Based on 1 Customer Validation
AP232 dihydrochloride is a selective U2AF1-UHM Inhibitor with an IC50 of 7.96 μM. AP232 dihydrochloride exhibits 2.8-24-fold selectivity against other UHM-containing proteins. AP232 dihydrochloride exerts anti-leukemia activity and shows higher activities in cell lines carrying splicing factor mutations. AP232 dihydrochloride can induce leukemia cells G2/M and G1 arrest, impair lysosome acidification, and inhibit autophagy. AP232 dihydrochloride can be used for the research of cancer, such as Leukemia.
For research use only. We do not sell to patients.
- Formula: C20H33Cl2N3O
- Molecular Weight:402.40
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
AP232 dihydrochloride inhibits the U2 auxiliary factor homology motif of U2AF1 (U2AF1-UHM) with an IC50 of 7.96 μM and exhibits 2.8-24-fold selectivity against other UHM-containing proteins (RBM39-UHM, SPF45-UHM, PUF60-UHM, U2AF2-UHM)[1].
AP232 (40 μM) dihydrochloride stabilizes endogenous full-length U2AF1 and reduces its sensitivity to Pronase in K562 cell[1].
AP232 (72 h) dihydrochloride inhibits cell growth in 6 leukemia cell lines (MV4-11, NKM-1, K562, MOLM13, HNT-34, MONO-MAC1)[1].
AP232 (5-30 μM, 24-48 h) dihydrochloride disrupts the cell cycle of leukemia cells[1].
AP232 (20 μM, 2 h) dihydrochloride reduces the proportion of functional lysosomes in NKM-1, K562, and MV4-11 cells[1].
AP232 (10-30 μM, 48 h) dihydrochloride inhibits autophagy in NKM-1 and K562 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:NKM-1 cells and K562 cells
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Concentration:5, 10, 20 and 30 μM
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Incubation Time:24 and 48 h
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Result:Increased the G2/M phase ratio at 48 h in NKM-1 cells.
Reduced the S phase and increased the sub-G1 phase at 24 h in K562 cells.
Increased the G1 phase while reduced the S and G2/M phases at 48 h in K562 cells.
Chemical Information
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Appearance Solid
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Molecular Weight 402.40
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Formula C20H33Cl2N3O
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SMILES
NC1(C)CCN(C(CCC2=CNC3=C2C(OCC)=CC=C3)C)CC1.Cl.Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Purity & Documentation
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Data Sheet (272 KB)
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SDS (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
AP232 dihydrochloride Related Classifications
HY-178155A Related Classifications
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