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Cat. No.: HY-178672

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ARCA

Name: ARCA
Brand: MedChemExpress
SKU: HY-178672
Availability: PreOrder

(Synonyms: Anti-Reverse cap analog; m2 7,3′O GpppG)

ARCA (Anti-Reverse cap analog; m₂^7,3'OGpppG) is a 5′-cap analog. ARCA carries a methyl group at the 3'-OH position of m7G, which ensures that the cap is ligated to the 5' end of mRNA in the correct orientation during in vitro transcription and prevents reverse ligation. ARCA enhances the translation efficiency and stability of mRNA. In human immature dendritic cells, both the translation efficiency and total protein expression of ARCA-capped mRNA are higher than those of conventional m7GpppG-capped mRNA. ARCA can be used in research related to cancer, infectious diseases and mRNA vaccines.

For research use only. We do not sell to patients.

CAS No.: 400806-46-4
Formula: C₂₂H₃₁N₁₀O₁₈P₃
Molecular Weight:816.46

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (267 KB)

ARCA Chemical Structure

CAS No.: 400806-46-4

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
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Biological Activity

Description

All DNA/RNA Synthesis Isoforms :

In Vitro

ARCA-capped mRNA serves as the reference for translational efficiency and total protein expression in human immature dendritic cells, with a total protein expression of 1.00 and translational efficiency of 1.00^[1].
ARCA enables proper cotranscriptional capping of in vitro transcribed mRNA by preventing reverse cap orientation, thereby supporting mRNA translation efficiency and stability^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 400806-46-4
Molecular Weight 816.46
Formula C₂₂H₃₁N₁₀O₁₈P₃
SMILES

O=C1C([N+](C)=CN2[C@H]3[C@H](O)[C@H](OC)[C@@H](COP(OP(OP(OC[C@H]4O[C@@H](N(C=N5)C6=C5C(NC(N)=N6)=O)[C@H](O)[C@@H]4O)(O)=O)(O)=O)([O-])=O)O3)=C2N=C(N)N1
Synonyms

Anti-Reverse cap analog; m2 7,3′O GpppG
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (267 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Kowalska J, et al. Synthesis, properties, and biological activity of boranophosphate analogs of the mRNA cap: versatile tools for manipulation of therapeutically relevant cap-dependent processes. Nucleic acids research. 2014;42(16):10245-64. [Content Brief]

[2]. Bednarczyk M, et al. Fluorescence-Based Activity Screening Assay Reveals Small Molecule Inhibitors of Vaccinia Virus mRNA Decapping Enzyme D9. ACS chemical biology. 2022 Jun 17;17(6):1460-1471.

[3]. Litvinova V R, et al. mRNA vaccine platform: mRNA production and delivery[J]. Russian Journal of Bioorganic Chemistry, 2023, 49(2): 220-235.

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
References

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ARCA

All DNA/RNA Synthesis Isoforms