Asn-Trp
Asn-Trp is an orally active antioxidant dipeptide. Asn-Trp inhibits oxidative stress, lipid peroxidation, and advanced glycation end product formation. Asn-Trp improves spatial memory and learning dysfunction. Asn-Trp can be obtained by computer-aided simulated hydrolysis of the yam tuber storage protein Dioscorin by pepsin (Pepsin) (HY-P1635), and can be detected in the pepsin hydrolysate by mass spectrometry after reversed-phase HPLC fractionation.
For research use only. We do not sell to patients.
- CAS No.: 74105-03-6
- Formula: C15H18N4O4
- Molecular Weight:318.33
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Asn-Trp (50-100 μM; 10 days) inhibits CML formation in the BSA/glucose model, reducing relative CML density to 58.8% at 100 μM[1].
Asn-Trp (50-200 μM; 24 h) protects HUVECs from MGO-induced cell death, restoring viability to 101% at 200 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Human umbilical vein endothelial cells (HUVECs)
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Concentration:50, 100, and 200 μM
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Incubation Time:24 h (pre-incubation); 24 h (MGO exposure)
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Result:At 50-200 μM, did not reduce HUVEC viability.
MGO exposure decreased HUVEC viability to about 53.4%.
Pre-incubation recovered cell viability to 70.9% at 50 μM, 85.7% at 100 μM, and 101% at 200 μM.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/c (male, 10 weeks old)[1]
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Dosage:10 mg/kg/day; 40 mg/kg/day
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Administration:p.o.; daily; 4 weeks
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Result:Plasma MDA at 8 weeks was lower in the 10 mg/kg/day and 40 mg/kg/day groups than in the Gal group.
In the Morris water maze reference memory task, 40 mg/kg/day reduced latency to reach the platform compared with Gal, with latency comparable to blank; 10 mg/kg/day did not.
Swimming speed was comparable across groups.
In the probe trial, 40 mg/kg/day increased crossings in the target quadrant compared with Gal, with crossings comparable to blank; 10 mg/kg/day did not.
Brain GSH was elevated by 40 mg/kg/day versus Gal; liver GSH was elevated by 10 mg/kg/day and 40 mg/kg/day versus Gal.
Brain MDA was reduced by 10 mg/kg/day and 40 mg/kg/day versus Gal and was comparable to blank; liver MDA was reduced by 40 mg/kg/day versus Gal.
ORAC activity in brain and liver tissues was elevated by 10 mg/kg/day and 40 mg/kg/day versus Gal.
40 mg/kg/day reduced argpyrimidine-modified AGEs in brain tissue versus Gal; 10 mg/kg/day and 40 mg/kg/day reduced CML-modified AGEs in brain tissue versus Gal, comparable to blank.
Chemical Information
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CAS No. 74105-03-6
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Molecular Weight 318.33
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Formula C15H18N4O4
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Sequence
Asn-Trp
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Sequence Shortening
NW
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Asn-Trp Related Classifications
HY-P12411 Related Classifications
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