2. GPCR/G Protein Neuronal Signaling
  3. mAChR
  4. ASP8302

ASP8302 is a positive and allosteric muscarinic M3 receptor modulator. ASP8302 improves voiding efficiency and reduced residual urine volume in two voiding dysfunction models. ASP8302 can be used for research of underactive bladder.

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ASP8302

ASP8302 構造式

CAS 番号 : 1839583-42-4

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 1194 在庫あり
Solution
10 mM * 1 mL in DMSO USD 1194 在庫あり
Solid
1 mg $360 在庫あり
5 mg $920 在庫あり
10 mg $1470 在庫あり
25 mg $2940 在庫あり
50 mg $4550 在庫あり
100 mg $6800 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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ASP8302 関連抗体

Top Publications Citing Use of Products

mAChR アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
mAChR1 mAChR2 mAChR3 mAChR4 mAChR5

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製品説明

ASP8302 is a positive and allosteric muscarinic M3 receptor modulator. ASP8302 improves voiding efficiency and reduced residual urine volume in two voiding dysfunction models. ASP8302 can be used for research of underactive bladder[1][2].

IC50 & Target[1]

mAChR3

 

体外実験

ASP8302 (0.1-1 μM) enhances Carbachol (HY-B1208)-induced intracellular Ca2+ mobilization in a concentration-dependent manner in CHO-K1 cells expressing rat muscarinic receptors[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ASP8302 関連抗体
体内実験

ASP8302 (0.1-1 mg/kg; i.v.) reduces residual urine volume and improves voiding efficiency in male SD rats with voiding dysfunction induced by Midodrine (HY-12749) and Atropine (HY-B1205)[2].
ASP8302 (1-3 mg/kg; i.v.) reduces residual urine volume and improves voiding efficiency in female Wistar rats with voiding dysfunction induced by bladder outlet obstruction[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
臨床実験
分子量

590.16

分子式

C26H32ClN7O3S2
CAS 番号
Appearance

Solid

Color

White to yellow

SMILES

O=C(C1=NC=C(N2C[C@@H](N(CC2)CCC(O)=O)C)N=C1)NC(S3)=NC(C4=CC(Cl)=CS4)=C3CN5[C@@H](CCC5)C
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : 100 mg/mL (169.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6945 mL 8.4723 mL 16.9446 mL
5 mM 0.3389 mL 1.6945 mL 3.3889 mL
10 mM 0.1694 mL 0.8472 mL 1.6945 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (4.24 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (4.24 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.6945 mL 8.4723 mL 16.9446 mL 42.3614 mL
5 mM 0.3389 mL 1.6945 mL 3.3889 mL 8.4723 mL
10 mM 0.1694 mL 0.8472 mL 1.6945 mL 4.2361 mL
15 mM 0.1130 mL 0.5648 mL 1.1296 mL 2.8241 mL
20 mM 0.0847 mL 0.4236 mL 0.8472 mL 2.1181 mL
25 mM 0.0678 mL 0.3389 mL 0.6778 mL 1.6945 mL
30 mM 0.0565 mL 0.2824 mL 0.5648 mL 1.4120 mL
40 mM 0.0424 mL 0.2118 mL 0.4236 mL 1.0590 mL
50 mM 0.0339 mL 0.1694 mL 0.3389 mL 0.8472 mL
60 mM 0.0282 mL 0.1412 mL 0.2824 mL 0.7060 mL
80 mM 0.0212 mL 0.1059 mL 0.2118 mL 0.5295 mL
100 mM 0.0169 mL 0.0847 mL 0.1694 mL 0.4236 mL
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ASP8302 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ASP83021839583-42-4ASP 8302ASP-8302mAChRMuscarinic acetylcholine receptorvoiding dysfunction modelunderactive bladderSD ratsCHO-K1Inhibitorinhibitorinhibit

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製品名:
ASP8302
製品番号:
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