Art. -Nr.: HY-119981

Ani9

2 Cited Publications

Kundenbewertung

Based on 2 publication(s) in Google Scholar

Ani9 is an ANO1 inhibitor, with an IC₅₀ of 77 nM. Ani9 can inhibit smooth muscle contractions in mice and is applicable in research related to diseases such as tumors.

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Reinheit: 99.12%
CAS. Nr.: 356102-14-2
Formel: C₁₇H₁₇ClN₂O₃
Molecular Weight:332.78

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Ani9 Chemical Structure

CAS. Nr.: 356102-14-2

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
Chemical Information
Publications (2)
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Ani9 is an ANO1 inhibitor, with an IC₅₀ of 77 nM. Ani9 can inhibit smooth muscle contractions in mice and is applicable in research related to diseases such as tumors^[1]^[2]^[3].

Cellular Effect

Cell Line	Type	Value	Description	References
U138-MG	GI₅₀	32.44 μM Compound: 3	Antiproliferative activity against human U138 cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay Antiproliferative activity against human U138 cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay	[PMID: 32906067]
U-251	GI₅₀	18.06 μM Compound: 3	Antiproliferative activity against human U251 cells overexpressing ANO1 assessed as reduction in cell viability in presence of temozolomide measured after 72 hrs by Celltiter-glo assay Antiproliferative activity against human U251 cells overexpressing ANO1 assessed as reduction in cell viability in presence of temozolomide measured after 72 hrs by Celltiter-glo assay	[PMID: 32906067]
U-251	GI₅₀	36.48 μM Compound: 3	Antiproliferative activity against human U251 cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay Antiproliferative activity against human U251 cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay	[PMID: 32906067]
U-87MG ATCC	GI₅₀	32.98 μM Compound: 3	Antiproliferative activity against human U87MG cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay Antiproliferative activity against human U87MG cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay	[PMID: 32906067]

In Vitro

Ani9 (0-30 μM, 20 min) does not affect the increase of cytoplasmic calcium induced by ATP in FRT cells^[1].
Ani9 (1-10 μM, 20 min) inhibits ANO1^[1].
Ani9 (0.03-10 μM, 20 min) inhibits CaCC current in PC3, Capan-1, and NHNE cells in a dose-dependent manner, with an IC₅₀ of 110 nM^[1].
Ani9 (0-10 μM, 24 h) highly expresses ANO1 in PC3 cells^[2].
Ani9 (0.01-10 μM, 48 h) inhibits weakly in prostate cancer, breast cancer, and pancreatic cancer cells^[2].
Ani9 (1 μM) inhibits Ca²⁺-activated Cl^- (Cl_ca) in portal vein smooth muscle of Cav1-KO mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:

PC3
Concentration:

0, 3, 10 μM
Incubation Time:

24 h
Result:

Expressed ANO1.

Cell Viability Assay^[2]

Cell Line:

PC3, MCF-7, BxPC3
Concentration:

0.01, 0.1, 1, 10 μM
Incubation Time:

48 h
Result:

Had a weak inhibitory effect on cells.

Chemical Information

CAS. Nr. 356102-14-2
Appearance Solid
Molecular Weight 332.78
Formel C₁₇H₁₇ClN₂O₃
Color White to off-white
SMILES

O=C(COC1=CC=C(C=C1C)Cl)N/N=C/C2=CC=CC=C2OC
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (2)

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Journal Impact Factor

Nat Commun

Noncanonical calcium-independent TRPM4 activation governs intestinal fluid homeostasis. [Abstract]

2026 Jan 8;17(1):1253. PMID: 41507162
Sci Rep

Identification of novel TMEM16A blockers through integrated virtual screening, molecular dynamics, and experimental studies. [Abstract]

2025 Apr 29;15(1):15065. PMID: 40301508

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : ≥ 125 mg/mL (375.62 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.0050 mL

15.0249 mL

30.0499 mL

5 mM

0.6010 mL

3.0050 mL

6.0100 mL

10 mM

0.3005 mL

1.5025 mL

3.0050 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Seo Y, et al. Ani9, A Novel Potent Small-Molecule ANO1 Inhibitor with Negligible Effect on ANO2. PLoS One. 2016 May 24;11(5):e0155771. [Content Brief]

[2]. Seo Y, et al. Synthesis and biological evaluation of novel Ani9 derivatives as potent and selective ANO1 inhibitors. Eur J Med Chem. 2018 Dec 5;160:245-255. [Content Brief]

[3]. Naoki Kawata, et al. Increased TMEM16A-Mediated Ca2+-Activated Cl− Currents in Portal Vein Smooth Muscle Cells of Caveolin 1-Deficient Mice. Biol. Pharm. Bull. 45, 1692–1698 (2022).

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.0050 mL	15.0249 mL	30.0499 mL	75.1247 mL
	5 mM	0.6010 mL	3.0050 mL	6.0100 mL	15.0249 mL
	10 mM	0.3005 mL	1.5025 mL	3.0050 mL	7.5125 mL
	15 mM	0.2003 mL	1.0017 mL	2.0033 mL	5.0083 mL
	20 mM	0.1502 mL	0.7512 mL	1.5025 mL	3.7562 mL
	25 mM	0.1202 mL	0.6010 mL	1.2020 mL	3.0050 mL
	30 mM	0.1002 mL	0.5008 mL	1.0017 mL	2.5042 mL
	40 mM	0.0751 mL	0.3756 mL	0.7512 mL	1.8781 mL
	50 mM	0.0601 mL	0.3005 mL	0.6010 mL	1.5025 mL
	60 mM	0.0501 mL	0.2504 mL	0.5008 mL	1.2521 mL
	80 mM	0.0376 mL	0.1878 mL	0.3756 mL	0.9391 mL
	100 mM	0.0300 mL	0.1502 mL	0.3005 mL	0.7512 mL

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Ani9

Membrane Transporter/Ion Channel Compound Library

Anti-Cancer Compound Library

Anti-Breast Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Mechanoreceptors Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Cysteine Targeted Covalent Library

Ion Channel Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) Ani9

Biologische Aktivität

Chemical Information

Publications (2)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Ani9

Membrane Transporter/Ion Channel Compound Library

Anti-Cancer Compound Library

Anti-Breast Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Mechanoreceptors Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Cysteine Targeted Covalent Library

Ion Channel Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) Ani9

Biologische Aktivität

Chemical Information

Publications (2)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Ani9 Related Classifications

HY-119981 Related Classifications