Basic green 4
Based on 1 Customer Validation
Basic green 4 (Malachite green) is a cationic dye that is widely used as a bactericide in aquaculture and is also commonly used in laboratories for the determination of phosphates. Basic green 4 exhibits high cytotoxicity and carcinogenicity to mammalian cells, specifically shown by its IC50 values of 2.03 µM for HEp-2 human laryngeal cells and 13.8 µM for Caco-2 human colorectal adenocarcinoma cells, and it can promote the development of liver tumors. The maximum absorbance wavelength is 621 nm.
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- Reinheit : 95.88%
- CAS. Nr.: 569-64-2
- Formel: C23H25ClN2
- Molecular Weight:364.91
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Speicherung:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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Biologische Aktivität
Beschreibung
In Vitro
Guide (The following is our recommended experimental protocol. This protocol is for reference only and should be modified according to your specific needs).
Basic Green 4 Measurement of Phosphate[1]
(1) Preparation of Basic Green 4 storage solution: Slowly add concentrated sulfuric acid (60 mL, d=1.84 g/L) to 300 mL of water. Cool the solution to room temperature and add 0.44 g of Basic Green 4.
(2) Preparation of Basic Green 4 working solution: Add 10 mL of the dye to 2.5 mL of 7.5% ammonium molybdate, then add 0.2 mL of 11% Tween 20.
(3) Mix 1 volume of Basic Green 4 with 4 volumes of the phosphate sample.
(4) Let it sit for ten minutes, then measure the absorbance at 630 nm.
Basic Green 4 Fungal Inhibition[2]
(1) Collect fish eggs using spawning mats in a pond environment and rinse off the mud with well water.
(2) Place the fish eggs in a petri dish and add Basic Green 4 at a concentration of 2 ppm for 60 minutes, or at a concentration of 10 ppm for 10 minutes.
(3) Discard the Basic Green 4 and rinse the fish eggs multiple times with well water.
(4) Place the fish eggs in a petri dish with clean, oxygenated water and incubate at 22°C (72°F). Observe the newly hatched fry for 24 hours to determine the survival rate after treatment. Each concentration test is repeated six times.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS. Nr. 569-64-2
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Appearance Solid
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Molecular Weight 364.91
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Formel C23H25ClN2
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Color Green to black
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SMILES
C/[N+](C)=C1C=C/C(C=C/1)=C(C2=CC=C(N(C)C)C=C2)\C3=CC=CC=C3.[Cl-]
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Synonyms
Malachite green
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 100 mg/mL (274.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 3.85 mg/mL (10.55 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Protokoll
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Carcinogenicity Bioassay
A carcinogenicity bioassay detects whether long-term exposure to a test substance increases benign or malignant tumor incidence, changes tumor spectrum, or shortens tumor latency in experimental animals; the classical rodent design exposes rats and/or mice to multiple dose levels for most of their lifespan, followed by complete necropsy and histopathologic diagnosis of neoplastic and non-neoplastic lesions. The readout is tumor incidence by organ, sex, species, dose group, and survival status; interpretation requires concurrent controls, dose-response assessment, survival-adjusted tumor statistics, and pathology review because mortality, spontaneous tumor background, and body-weight effects can influence apparent tumor rates.
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Reinheit & Dokumentation
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Data Sheet (274 KB)
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SDS (862 KB)
- English - EN (862 KB)
- Français - FR (862 KB)
- Deutsch - DE (862 KB)
- Norwegian - NO (862 KB)
- Español - ES (862 KB)
- Swedish - SV (862 KB)
- Italian - IT (862 KB)
- Korean - KR (862 KB)
- Portuguese - PT (862 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 2.7404 mL | 13.7020 mL | 27.4040 mL | 68.5100 mL |
| 5 mM | 0.5481 mL | 2.7404 mL | 5.4808 mL | 13.7020 mL | |
| 10 mM | 0.2740 mL | 1.3702 mL | 2.7404 mL | 6.8510 mL | |
| DMSO | 15 mM | 0.1827 mL | 0.9135 mL | 1.8269 mL | 4.5673 mL |
| 20 mM | 0.1370 mL | 0.6851 mL | 1.3702 mL | 3.4255 mL | |
| 25 mM | 0.1096 mL | 0.5481 mL | 1.0962 mL | 2.7404 mL | |
| 30 mM | 0.0913 mL | 0.4567 mL | 0.9135 mL | 2.2837 mL | |
| 40 mM | 0.0685 mL | 0.3426 mL | 0.6851 mL | 1.7128 mL | |
| 50 mM | 0.0548 mL | 0.2740 mL | 0.5481 mL | 1.3702 mL | |
| 60 mM | 0.0457 mL | 0.2284 mL | 0.4567 mL | 1.1418 mL | |
| 80 mM | 0.0343 mL | 0.1713 mL | 0.3426 mL | 0.8564 mL | |
| 100 mM | 0.0274 mL | 0.1370 mL | 0.2740 mL | 0.6851 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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