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Art. -Nr.: HY-114208

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BI-9321

BI-9321, a chemical probe, is a potent, selective and cellular active nuclear receptor-binding SET domain 3 (NSD3)-PWWP1 domain antagonist with a K_d value of 166 nM. BI-9321 is inactive against NSD2-PWWP1 and NSD3-PWWP2. BI-9321 specifically disrupts histone interactions of the NSD3-PWWP1 domain with an IC₅₀ of 1.2 μM in U2OS cells.

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CAS. Nr.: 2387510-86-1
Formel: C₂₂H₂₁FN₄
Molecular Weight:360.43

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (269 KB)

BI-9321 Chemical Structure

CAS. Nr.: 2387510-86-1

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Protokoll (1)
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

NSD3

Alle Histone Methyltransferase Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
MOLM-13	IC₅₀	0.139 μM Compound: 18; BI-9321	Antiproliferative activity against human MOLM-13 cells assessed as cell growth inhibition measured after 6 days in presence of JQ1 by RealTime-Glow MT cell viability assay Antiproliferative activity against human MOLM-13 cells assessed as cell growth inhibition measured after 6 days in presence of JQ1 by RealTime-Glow MT cell viability assay	[PMID: 37182335]
MOLM-13	IC₅₀	26.8 μM Compound: 18; BI-9321	Antiproliferative activity against human MOLM-13 cells assessed as cell growth inhibition measured after 6 days by RealTime-Glow MT cell viability assay Antiproliferative activity against human MOLM-13 cells assessed as cell growth inhibition measured after 6 days by RealTime-Glow MT cell viability assay	[PMID: 37182335]
MOLM-13	IC₅₀	26.8 μM Compound: 39; BI-9321	Antiproliferative activity against human MOLM-13 cells assessed as reduction in cell growth incubated for 48 hrs by MTT assay Antiproliferative activity against human MOLM-13 cells assessed as reduction in cell growth incubated for 48 hrs by MTT assay	[PMID: 38805937]
MOLM-13	IC₅₀	26.8 μM Compound: BI-9321	Inhibition of cell proliferation of human MOLM-13 cells incubated for 6 days by Real-time Glow MT assay Inhibition of cell proliferation of human MOLM-13 cells incubated for 6 days by Real-time Glow MT assay	[PMID: 38412693]

In Vitro

BI-9321 is targeting the methyl-lysine binding site of the PWWP1 domain with sub-micromolar in vitro activity and cellular target engagement at 1 μM. As a single agent, BI-9321 downregulates Myc messenger RNA expression and reduces proliferation in MOLM-13 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

Chemical Information

CAS. Nr. 2387510-86-1
Molecular Weight 360.43
Formel C₂₂H₂₁FN₄
SMILES

NCC1=CC(C)=C(C2=C(C3=CC=NC4=CC(F)=CC=C34)N(C)C=N2)C(C)=C1
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Protokoll

All Cell Biology

Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (269 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Böttcher J, et al. Fragment-based discovery of a chemical probe for the PWWP1 domain of NSD3. Nat Chem Biol. 2019 Aug;15(8):822-829. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

MOQ

Minimum order quantity

100 mg

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