Art. -Nr.: HY-N6684

Deoxynivalenol

(Synonyms: Vomitoxin)

18 Cited Publications

Kundenbewertung

Based on 18 publication(s) in Google Scholar

Deoxynivalenol, an orally active mycotoxin of the trichothecenes family, crosses the intestinal mucosa by a paracellular pathway through the tight junctions. The Deoxynivalenol transport is not affected by P-glycoprotein (PgP) or multidrug resistance-associated proteins (MRPs) inhibitors.

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Reinheit: 99.90%
CAS. Nr.: 51481-10-8
Formel: C₁₅H₂₀O₆
Molecular Weight:296.32

Speicherung:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

Deoxynivalenol Chemical Structure

CAS. Nr.: 51481-10-8

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Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (18)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Cellular Effect

Cell Line	Type	Value	Description	References
COLO 201	IC₅₀	86 μM Compound: 2; DON	Antiproliferative activity against human COLO201 cells after 24 hrs by WST-1 assay Antiproliferative activity against human COLO201 cells after 24 hrs by WST-1 assay	[PMID: 30797671]
K562	EC₅₀	1010 nM Compound: DON	Cytotoxicity against human K562 cells by MTT assay Cytotoxicity against human K562 cells by MTT assay	[PMID: 18327910]

In Vitro

Deoxynivalenol (0-2 μg/mL, 24 h) provokes the phosphorylation of the mitogen-activated protein kinases (MAPKs) Erk1/2, p38 and SAPK/JNK in Caco-2 cells^[1].
Deoxynivalenol (100-4000 ng/mL, 24-72 h) reduces the viability of IPEC-1 and IPEC-J2 cells after 48 h and 72 h incubation at the doses of 500-4000 ng/mL^[2].
Deoxynivalenol (0-4000 ng/mL, 24-72 h) increases BrdU incorporation at 200 ng/mL and decreases BrdU incorporation at 2000-4000 ng/mL in IPEC-1 and IPEC-J2 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

IPEC-1 and IPEC-J2 cells
Concentration:

100-4000 ng/mL
Incubation Time:

24-72 h
Result:

Reduced the cell count in a time and dose dependent.
Increased LDH signals in IPEC-1 after 48 h and 72 h incubation with 2000 ng/mL and in IPEC-J2 after 48 h incubation with 2000 ng/mL.
Reduced NR uptake in IPEC-1 and IPEC-J2 cells at the doses of 2000 ng/mL.
Decreased the MTT signal in IPEC-1 to 60% and 40% of the control after 48 h and 72 h at the doses of 2000 ng/mL.
Decreased the MTT signal in IPEC-J2 to 70% and 58% of the control after 48 h and 72 h at the doses of 2000 ng/mL.

Cell Proliferation Assay^[1]

Cell Line:

Caco-2 cells
Concentration:

0-10 μg/mL
Incubation Time:

48 h
Result:

Inhibited the proliferation of cells in a dose-dependent manner.

Cell Cycle Analysis^[2]

Cell Line:

IPEC-1 and IPEC-J2 cells
Concentration:

200-2000 ng/mL
Incubation Time:

24-72 h
Result:

Increased percentages of pre-G1, S and G2/M phases and decreased of the G0/G1 phase in response to 2000 ng/mL after 48 h in IPEC-1 cells.
Decreased the G0/G1 phase in IPEC-J2 cell at the doses of 2000 ng/mL after 48 h.
Increased the percentage of pre-G1 in IPEC-1 cells after 72 h.
Increased the G2/M phase in IPEC-1 and IPEC-J2 cells at the doses of 2000 ng/mL after 72 h.

Apoptosis Analysis^[2]

Cell Line:

IPEC-1 and IPEC-J2 cells
Concentration:

200-2000 ng/mL
Incubation Time:

6-72 h
Result:

Increased the caspase 3 activity after 6 h, 8 h, 48 h and 72 h of incubation with 2000 ng/mL.
Reduced the caspase 3 activity to the control level after 24 h of incubation with 2000 ng/mL.

Western Blot Analysis^[1]

Cell Line:

Caco-2 cells
Concentration:

2 μg/mL
Incubation Time:

5-240 min
Result:

Increased the phosphorylation of Erk1/Erk2 during 5 min.
Increased the phosphorylation of p38 from 5 to 30 min.

Western Blot Analysis^[2]

Cell Line:

IPEC-1 and IPEC-J2 cells
Concentration:

200-2000 ng/mL
Incubation Time:

48 h
Result:

Reduced the protein level of ZO-1 at the doses of 2000 ng/mL.

In Vivo

Deoxynivalenol (0-5 mg/kg, i.g., daily, 14 days) increases the number of pregnant rats with excessive salivation in a dose-related manner^[3].
Deoxynivalenol (0-5 mg/kg, i.g., daily, 14 days) causes abnormal sternum development in fetuses rats at a dose of 5 mg/kg^[3].
Deoxynivalenol (10-1000 mg/kg; p.o., i.p.; once time) is estimated to be 78 mg/kg (p.o.) and 49 mg/kg (i.p.) of LD50 values in the B6C3F1 mouse^[4].
Deoxynivalenol (2-20 mg/kg, p.o., daily, 4 weeks) increases in plasma in a dose-dependent manner and reduces weight gain in B6C3F1 mouse^[5].
1.19
Pharmacokinetic Analysis in B6C3F1 Mice Model^[5]

Route	Dose (mg/kg)	Plasma T_1/2α (min)	Plasma T_1/2β (h)	Liver T_1/2α (min)	Liver T_1/2β (h)	Kidney T_1/2α (min)	Kidney T_1/2β (h)	Spleen T_1/2α (min)	Spleen T_1/2β (h)	Heart T_1/2α (min)	Heart T_1/2β (h)
p.o.	25	20.4	11.8	22	19	47	20.9	29	9	41	12.3

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Pregnant rats^[3]
Dosage:

0-5 mg/kg
Administration:

i.g., daily, 14 days
Result:

Reduced feed consumption and mean body weight gain in the 5 mg/kg group .
Reduced feed consumption in the 2.5 mg/kg group during 8-14 days.
Decreased the number of viable fetuses at 5 mg/kg.
Reduced fetal body weight and crown-rump length at 2.5 and 5 mg/kg.
Increased the incidence of runts and the average number of fetuses per litter with internal anomalies at 2.5 and 5 mg/kg.
Increased the incidence of misaligned and fused sternebrae at 5 mg/kg.
Increased the ratios of liver-to-body-weight and kidneys-to-body-weight.

Chemical Information

CAS. Nr. 51481-10-8
Appearance Solid
Molecular Weight 296.32
Formel C₁₅H₂₀O₆
Color White to off-white
SMILES

O=C(C(C)=C1)[C@@H](O)[C@@]2(CO)[C@]1([H])O[C@]3([H])[C@H](O)C[C@@]2(C)[C@@]34OC4
Synonyms

Vomitoxin
Structure Classification
- Terpenoids
- Sesquiterpenes
Initial Source
- Microorganisms
Fusarium specie
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (18)

All Cell Proliferation/Viability AssayHistological Imaging/StainingRT-PCRWBIF

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Journal Impact Factor

Environ Int

Low dosage exposure of deoxynivalenol and copper synergistically enhanced the intestinal toxicity. [Abstract]

2025 Oct 28:205:109891. PMID: 41172574

Deoxynivalenol purchased from MedChemExpress. Usage Cited in: Environ Int. 2025 Oct 28:205:109891. [Abstract]

Deoxynivalenol (DON, 0.5-4 μM; 24 h) effects on cell viability of IPEC-J2 cells.
Cell Commun Signal

Tubacin alleviate the reproductive toxicity of deoxynivalenol in mouse oocytes and zygotes via strengthening microtubule stability. [Abstract]

2025 Oct 3;23(1):417. PMID: 41044645
Free Radic Biol Med

2026 Jun 27:S0891-5849(26)00902-0. PMID: 42364855
Ecotoxicol Environ Saf

Deoxynivalenol-mediated kidney injury via endoplasmic reticulum stress in mice. [Abstract]

2024 Nov 1:286:117243. PMID: 39447294

Deoxynivalenol purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2024 Nov 1:286:117243. [Abstract]

Histological characterizations in the kidney sections were evaluated. The results showed that compared with the control group, Deoxynivalenol (DON, 2.5-5 mg/kg; i.p.; single dose) treatment increased inflammatory cell infiltration, hemorrhage, tubular degeneration, and glomerular atrophy in the kidneys of mice. (magnification:×400, scale bar: 50 μm). G: glomerulus, P: proximal tubule, D: distal tubule; Hemorrhage (Δ), inflammatory cell infiltration (↓), tubular degradation (⇒), glomerular atrophy (▲).

Deoxynivalenol purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2024 Nov 1:286:117243. [Abstract]

Deoxynivalenol (DON, 2.5–5 mg/kg; i.p., single dose) significantly upregulated the mRNA expression levels of the proinflammatory cytokines IL-6, IL-8, IL-1β, and TNF-α in mouse renal tissues.

Deoxynivalenol purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2024 Nov 1:286:117243. [Abstract]

Protein expression levels of Caspase12, Cleaved-caspase3, Bax, and Bcl-2 were assessed in the kidneys of mice treated with Deoxynivalenol (DON, i.p.; single dose) at either 2.5 or 5 mg/kg BW for 3 hours.
Talanta

Nanobody-based magnetic chemiluminescence immunoassay for one-pot detection of ochratoxin A. [Abstract]

2023 Jun 1:258:124388. PMID: 36921368
Ecotoxicol Environ Saf

Dietary supplementation with selenium nanoparticles-enriched Lactobacillus casei ATCC 393 alleviates intestinal barrier dysfunction of mice exposed to deoxynivalenol by regulating endoplasmic reticulum stress and gut microbiota. [Abstract]

2022 Dec 15:248:114276. PMID: 36371888

Deoxynivalenol purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2022 Dec 15:248:114276. [Abstract]

Deoxynivalenol (DON) exposure results in the decrease of the expression levels of occludin and claudin-1 in duodenum of mice.
Microchem J

2025 Dec 11;220:116425.
Commun Biol

Deoxynivalenol induces ferroptosis via inhibiting glycolysis-H3K18la-STEAP3 axis to promote ovary damage in piglets. [Abstract]

2025 Nov 20;8(1):1626. PMID: 41266595
Toxicology

NNMT is involved in deoxynivalenol-induced hepatocyte toxicity via promoting ferroptosis. [Abstract]

2025 May:513:154084. PMID: 39983889
Toxicology

CYP2E1 mediated deoxynivalenol-induced hepatocyte toxicity by regulating ferroptosis. [Abstract]

2024 Aug 13:153923. PMID: 39147090
Toxicology

Resveratrol protects against deoxynivalenol-induced ferroptosis in HepG2 cells. [Abstract]

2023 Aug 1:494:153589. PMID: 37419272
Poult Sci

Quercetin attenuates deoxynivalenol-induced muscle developmental disorders in broilers by modulating the PI3K/Akt/mTOR pathway and reducing apoptosis. [Abstract]

2026 Mar 5;105(6):106740. PMID: 41850073
Toxins

Deoxynivalenol-Induced Spleen Toxicity in Mice: Inflammation, Endoplasmic Reticulum Stress, Macrophage Polarization, and the Dysregulation of LncRNA Expression. [Abstract]

2024 Oct 9;16(10):432. PMID: 39453208
Microb Pathog

Nano-selenium alleviates the pyroptosis and inflammation of ST cells induced by deoxynivalenol via ROS/NF-κB/NLRP3 axis. [Abstract]

2026 Jul:216:108527. PMID: 42070668
Biol Reprod

Melatonin alleviates deoxynivalenol-induced apoptosis of human granulosa cells by reducing mutually accentuated FOXO1 and ER stress‡. [Abstract]

2021 Aug 3;105(2):554-566. PMID: 33907797
Toxicon

18beta-glycyrrhetinic acid alleviates deoxynivalenol-induced hepatotoxicity by inhibiting GPX4-dependent ferroptosis. [Abstract]

2025 Jan 9:108228. PMID: 39798898
Vet Sci

The Alleviating Effect of Taxifolin on Deoxynivalenol-Induced Damage in Porcine Intestinal Epithelial Cells. [Abstract]

2024 Mar 30;11(4):156. PMID: 38668423
SSRN

2025 Dec 31.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (337.47 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 30 mg/mL (101.24 mM; Need ultrasonic and warming)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.3747 mL

16.8736 mL

33.7473 mL

5 mM

0.6749 mL

3.3747 mL

6.7495 mL

10 mM

0.3375 mL

1.6874 mL

3.3747 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (8.44 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (8.44 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (293 KB)
SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Sergent T, et al. Deoxynivalenol transport across human intestinal Caco-2 cells and its effects on cellularmetabolism at realistic intestinal concentrations. Toxicol Lett. 2006 Jul 1;164(2):167-76. [Content Brief]

[2]. Diesing AK, et al. Mycotoxin deoxynivalenol (DON) mediates biphasic cellular response in intestinal porcine epithelial cell lines IPEC-1 and IPEC-J2. Toxicol Lett. 2011 Jan 15;200(1-2):8-18. [Content Brief]

[3]. Collins TF, et al. Effects of deoxynivalenol (DON, vomitoxin) on in utero development in rats. Food Chem Toxicol. 2006 Jun;44(6):747-57. [Content Brief]

[4]. Forsell JH, et al. Comparison of acute toxicities of deoxynivalenol (vomitoxin) and 15-acetyldeoxynivalenol in the B6C3F1 mouse. Food Chem Toxicol. 1987 Feb;25(2):155-62. [Content Brief]

[5]. Pestka JJ, et al. Immunochemical assessment of deoxynivalenol tissue distribution following oral exposure in the mouse. Toxicol Lett. 2008 May 5;178(2):83-7. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
Ethanol / DMSO	1 mM	3.3747 mL	16.8736 mL	33.7473 mL	84.3682 mL
	5 mM	0.6749 mL	3.3747 mL	6.7495 mL	16.8736 mL
	10 mM	0.3375 mL	1.6874 mL	3.3747 mL	8.4368 mL
	15 mM	0.2250 mL	1.1249 mL	2.2498 mL	5.6245 mL
	20 mM	0.1687 mL	0.8437 mL	1.6874 mL	4.2184 mL
	25 mM	0.1350 mL	0.6749 mL	1.3499 mL	3.3747 mL
	30 mM	0.1125 mL	0.5625 mL	1.1249 mL	2.8123 mL
	40 mM	0.0844 mL	0.4218 mL	0.8437 mL	2.1092 mL
	50 mM	0.0675 mL	0.3375 mL	0.6749 mL	1.6874 mL
	60 mM	0.0562 mL	0.2812 mL	0.5625 mL	1.4061 mL
	80 mM	0.0422 mL	0.2109 mL	0.4218 mL	1.0546 mL
	100 mM	0.0337 mL	0.1687 mL	0.3375 mL	0.8437 mL

Keywords

Deoxynivalenol

18 Cited Publications

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Deoxynivalenol

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Publications Citing Use of MedChemExpress (MCE) Deoxynivalenol

Biologische Aktivität

Chemical Information

Publications (18)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Deoxynivalenol

Membrane Transporter/Ion Channel Compound Library

Natural Product Library

Covalent Screening Library

Terpenoids Library

Orally Active Compound Library

Traditional Chinese Medicine Active Compound Library

Microbial Metabolite Library

Food-Sourced Compound Library

Human Metabolite Library

Heterocyclic Compound Library

Cysteine Targeted Covalent Library

Anti-Drug-Resistant Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Covalent Screening Library Plus

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

RO5 Drug-like Natural Product Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Mass Spectrometry Human Metabolite Library

Biotoxin Library

Diarrhea-Related Traditional Chinese Medicine Active Compound Library

Mongolian Medicine Compound Library

Natural Product Diversity Scaffold Library

Fungal-Sourced Compound Library

Mass Spectrometry Natural Product Library

Publications Citing Use of MedChemExpress (MCE) Deoxynivalenol

Biologische Aktivität

Chemical Information

Publications (18)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Deoxynivalenol Related Classifications

HY-N6684 Related Classifications