Art. -Nr.: HY-W015635

Diallyl disulfide

Name: Diallyl disulfide
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Diallyl disulfide is an orally active decomposition product of allicin and has a pungent odor. Diallyl disulfide can act as a hydrogen sulfide donor. Diallyl disulfide has inhibitory activity against squalene monooxygenase with an IC₅₀ of 400 μM. Diallyl disulfide can alleviate the cytotoxicity and apoptosis of intestinal and liver cells induced by Ethyl Carbamate (HY-B1207). Diallyl disulfide can prevent emphysema induced by cigarette smoke. Diallyl disulfide also has anti-colon cancer and anti-inflammatory neurogenic activities, and can reverse the depressive-like behavior of mice.

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Reinheit : 99.8%
CAS. Nr.: 2179-57-9
Formel: C₆H₁₀S₂
Molecular Weight:146.27

Speicherung:

4°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Diallyl disulfide Chemical Structure

CAS. Nr.: 2179-57-9

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Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	100 μM Compound: Diallyl disulfide	Antiproliferative activity against human A549 cells Antiproliferative activity against human A549 cells	[PMID: 30245394]
Caco-2	IC₅₀	100 μM Compound: Diallyl disulfide	Antiproliferative activity against human Caco2 cells Antiproliferative activity against human Caco2 cells	[PMID: 30245394]
CHO	EC₅₀	7.55 μM Compound: 3	Agonist activity at human TRPA1 channel expressed in CHO cells assessed as increase in intracellular calcium levels Agonist activity at human TRPA1 channel expressed in CHO cells assessed as increase in intracellular calcium levels	[PMID: 20356305]
DLD-1	IC₅₀	100 μM Compound: Diallyl disulfide	Antiproliferative activity against human DLD1 cells Antiproliferative activity against human DLD1 cells	[PMID: 30245394]
HCT-15	IC₅₀	100 μM Compound: Diallyl disulfide	Antiproliferative activity against human HCT15 cells Antiproliferative activity against human HCT15 cells	[PMID: 30245394]
MDCK	IC₅₀	1.82 mM Compound: 2	Cytotoxicity against MDCK cells assessed as decrease in cell viability after 16 hrs by tetrazolium salt WST-1 assay Cytotoxicity against MDCK cells assessed as decrease in cell viability after 16 hrs by tetrazolium salt WST-1 assay	[PMID: 23163425]
SK-MEL-2	IC₅₀	100 μM Compound: Diallyl disulfide	Antiproliferative activity against human SK-MEL-2 cells Antiproliferative activity against human SK-MEL-2 cells	[PMID: 30245394]

In Vitro

Diallyl disulfide (30-240 μM; 12-48 h) suppresses HT-29 cell growth^[2].
Diallyl disulfide (30-120 μM; 24 h) induces cell cycle arrest in the G2/M-phase in HT-29 cells in a concentration-independent manner^[2].
Diallyl disulfide (120 μM) induces the mRNA levels of ARPC3, SPINT2, SOD1, p21 and TACSTD2^[2].
Diallyl disulfide (10-120 μM; 24 h) can protect Caco-2 and HepG2 cells from the cytotoxicity induced by ethyl carbamate. Compared with the group exposed to ethyl carbamate alone, it can significantly increase the cell survival rate^[5].
Diallyl disulfide (10-40 μM; 24 h) can reduce the ROS production induced by carbamate exposure for 24 hours in Caco-2 and HepG2 cells^[5].
Diallyl disulfide (10-40 μM; 24 h) can protect Caco-2 and HepG2 cells from the apoptosis and necrosis induced by carbamate. Compared with the group exposed to carbamate alone, it can significantly reduce the proportion of apoptotic and necrotic cells^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

HT-29 cells
Concentration:

30, 60, 120 and 240 μM
Incubation Time:

12, 24, 48 h
Result:

Suppressed HT-29 cell growth by 5.3, 23.1, 45.6 and 68.3% with 30, 60, 120 and 240 µM for 24 h, respectively.

Cell Cycle Analysis^[2]

Cell Line:

HT-29 cells
Concentration:

30, 60, 120 μM
Incubation Time:

24 h
Result:

The percentage of cells in the G2/M-phase was found to be 23.6, 25.9, 39.7 and 61.0% with 30, 60, 120 and 240 µM, respectively.

Apoptosis Analysis^[5]

Cell Line:

human colorectal adenocarcinoma (Caco-2) and hepatocarcinoma (HepG2) cells
Concentration:

10, 20 and 40 μM
Incubation Time:

24 h
Result:

Reduced ethyl carbamate-induced apoptosis (increased by 20% vs negative control) and necrosis (increased by 5% vs negative control) in Caco-2 cells by approximately 70% vs ethyl carbamate alone. Reduced ethyl carbamate-induced apoptosis and necrosis (increased by 10% vs negative control) in HepG2 cells compared to ethyl carbamate alone.
Did not induce apoptosis or necrosis in either cell line vs solvent control when used alone.

In Vivo

Diallyl disulfide (40-80 mg/kg; i.p.; pre-treatment 1 h) has a prophylactic effect in LPS-induced depression-like behaviors in mice^[3].
Diallyl disulfide (30-90 mg/kg; i.g.; three times/week for the last 30 days) dose-dependently reduces cigarette smoke (CS) -induced leukocyte infiltration into the airways and improves lung histology in C57BL/6 mice were exposed to CS for 60 consecutive days^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male C57BL6/J mice (6-8 weeks)^[3]
Dosage:

40, 80 mg/kg
Administration:

IP; pre-treatment 1 h before a single LPS injection (100 μg/kg)
Result:

Prevented LPS-induced increases in immobility time in the TST and FST in mice.
Did not affect the locomotor activity in mice with or without LPS treatment.

Related Protocols :

Chemical Information

CAS. Nr. 2179-57-9
Appearance Liquid (Density: 1.010 g/cm³)
Molecular Weight 146.27
Formel C₆H₁₀S₂
Color Colorless to light yellow
SMILES

C=CCSSCC=C
Structure Classification
- Others
Initial Source
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Publications (1)

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Journal Impact Factor

bioRxiv

2024 August 18.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (683.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

6.8367 mL

34.1834 mL

68.3667 mL

5 mM

1.3673 mL

6.8367 mL

13.6733 mL

10 mM

0.6837 mL

3.4183 mL

6.8367 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (17.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (17.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (17.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (481 KB)
- English - EN (481 KB)
- Français - FR (481 KB)
- Deutsch - DE (481 KB)
- Norwegian - NO (481 KB)
- Español - ES (481 KB)
- Swedish - SV (481 KB)
- Italian - IT (481 KB)
- Korean - KR (481 KB)
- Portuguese - PT (481 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Gupta N, et al. Garlic and garlic-derived compounds inhibit human squalene monooxygenase. J Nutr. 2001;131(6):1662-1667. [Content Brief]

[2]. You-Sheng Huang, et al. Diallyl disulfide inhibits the proliferation of HT-29 human colon cancer cells by inducing differentially expressed genes. Mol Med Rep. 2011 May-Jun;4(3):553-9. [Content Brief]

[3]. Xiaoyou Wei, et al. Acute Diallyl Disulfide Administration Prevents and Reveres Lipopolysaccharide-Induced Depression-Like Behaviors in Mice via Regulating Neuroinflammation and Oxido-Nitrosative Stress. Inflammation. 2021 Aug;44(4):1381-1395. [Content Brief]

[4]. Aline de Oliveira Pontes Cardoso, et al. Diallyl disulfide prevents cigarette smoke-induced emphysema in mice. Pulm Pharmacol Ther. 2021 Aug:69:102053. [Content Brief]

[5]. Sanchez CA, et al. Diallyl Disulfide Reduces Ethyl Carbamate-Induced Cytotoxicity and Apoptosis in Intestinal and Hepatic Cells. Chem Res Toxicol. 2025 Apr 21;38(4):623-634. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	6.8367 mL	34.1834 mL	68.3667 mL	170.9168 mL
	5 mM	1.3673 mL	6.8367 mL	13.6733 mL	34.1834 mL
	10 mM	0.6837 mL	3.4183 mL	6.8367 mL	17.0917 mL
	15 mM	0.4558 mL	2.2789 mL	4.5578 mL	11.3945 mL
	20 mM	0.3418 mL	1.7092 mL	3.4183 mL	8.5458 mL
	25 mM	0.2735 mL	1.3673 mL	2.7347 mL	6.8367 mL
	30 mM	0.2279 mL	1.1394 mL	2.2789 mL	5.6972 mL
	40 mM	0.1709 mL	0.8546 mL	1.7092 mL	4.2729 mL
	50 mM	0.1367 mL	0.6837 mL	1.3673 mL	3.4183 mL
	60 mM	0.1139 mL	0.5697 mL	1.1394 mL	2.8486 mL
	80 mM	0.0855 mL	0.4273 mL	0.8546 mL	2.1365 mL
	100 mM	0.0684 mL	0.3418 mL	0.6837 mL	1.7092 mL

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Keywords

Diallyl disulfide

1 Cited Publications

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Diallyl disulfide

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