FM19G11
Based on 1 Customer Validation
FM19G11 is a hypoxia-inducible factor-1-alpha (HIF-1α) inhibitor, and it inhibits hypoxia-induced luciferase activity with an IC50 of 80 nM in HeLa cells. FM19G11 modulates other signaling pathways, including mTOR and PI3K/Akt/eNOS, when the HIF-1α pathway is inactivated under normoxic conditions.
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- Reinheit : 98.39%
- CAS. Nr.: 329932-55-0
- Formel: C23H17N3O8
- Molecular Weight:463.40
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Speicherung:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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Biologische Aktivität
Beschreibung
IC50 & Target
HIF-1α[1]
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| HeLa | IC50 |
2.6 μM
Compound: 70; FMG19G11
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Inhibition of HIF-1alpha in human HeLa cells after 6 hrs by luciferase reporter gene assay
Inhibition of HIF-1alpha in human HeLa cells after 6 hrs by luciferase reporter gene assay
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[PMID: 30682255] |
In Vitro
FM19G11 (30-300 nM) inhibits HIFα proteins in the HeLa cell lines[1].
FM19G11 (500 nM) promotes oligodendrocyte differentiation under hypoxia[1].
FM19G11 (300 nM; 3 days) suppresses the mRNA levels of O6-methylguanine DNA-methyltransferase (MGMT) significantly in hypoxic GBM‐XD, hypoxic T98G, and normoxic T98G cells[2].
M19G11 (300 nM; 3 days) significantly enhances the pro‐apoptotic effect of temozolomide (TMZ), although FM19G11 does not induce apoptosis by itself[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:GBM‐XD and T98G cells
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Concentration:300 nM
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Incubation Time:3 days
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Result:Had no cytotoxicity by itself.
Enhanced the cytotoxicity of TMZ in hypoxic GBM-XD cells, hypoxic T98G cells, and normoxic T98G cells.
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Cell Line:GBM‐XD and T98G cells
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Concentration:300 nM
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Incubation Time:3 days
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Result:Suppressed MGMT expression significantly in both cell lines in hypoxic culture.
Downregulated MGMT expression substantially inT98G cells in normoxic culture.
In Vivo
FM19G11 ( intramedullary injection; 8 weeks) induces the expression of GAP43, an axon growth marker, and RIP, a marker for myelinated oligodendrocytes at the injury[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 329932-55-0
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Appearance Solid
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Molecular Weight 463.40
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Formel C23H17N3O8
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Color White to light yellow
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SMILES
O=C(OCC(C1=CC=C(C)C=C1)=O)C2=CC=CC(NC(C3=CC=C([N+]([O-])=O)C=C3[N+]([O-])=O)=O)=C2
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 100 mg/mL (215.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Protokoll
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Dual Luciferin reporter gene assay
Luciferin reporter gene assay is a reporting system to detect the activity of Firefly Luciferase using luciferin as a substrate, which is often used in the research of miRNA target gene verification and promoter transcriptive activity regulation. Dual luciferase usually refers to Firefly luciferase and Renilla luciferase.
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Reinheit & Dokumentation
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Data Sheet (273 KB)
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SDS (544 KB)
- English - EN (544 KB)
- Français - FR (544 KB)
- Deutsch - DE (544 KB)
- Norwegian - NO (544 KB)
- Español - ES (544 KB)
- Swedish - SV (544 KB)
- Italian - IT (544 KB)
- Korean - KR (544 KB)
- Portuguese - PT (544 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Victoria MM, et, al. FM19G11, a new hypoxia-inducible factor (HIF) modulator, affects stem cell differentiation status. J Biol Chem. 2010 Jan 8; 285(2): 1333-42. [Content Brief]
[2]. You CG, et, al. FM19G11 inhibits O 6 -methylguanine DNA-methyltransferase expression under both hypoxic and normoxic conditions. Cancer Med. 2018 May 15; 7(7): 3292-3300. [Content Brief]
[3]. Ana AA, et, al. FM19G11 and Ependymal Progenitor/Stem Cell Combinatory Treatment Enhances Neuronal Preservation and Oligodendrogenesis after Severe Spinal Cord Injury. Int J Mol Sci. 2018 Jan 9; 19(1): 200. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1580 mL | 10.7898 mL | 21.5796 mL | 53.9491 mL |
| 5 mM | 0.4316 mL | 2.1580 mL | 4.3159 mL | 10.7898 mL | |
| 10 mM | 0.2158 mL | 1.0790 mL | 2.1580 mL | 5.3949 mL | |
| 15 mM | 0.1439 mL | 0.7193 mL | 1.4386 mL | 3.5966 mL | |
| 20 mM | 0.1079 mL | 0.5395 mL | 1.0790 mL | 2.6975 mL | |
| 25 mM | 0.0863 mL | 0.4316 mL | 0.8632 mL | 2.1580 mL | |
| 30 mM | 0.0719 mL | 0.3597 mL | 0.7193 mL | 1.7983 mL | |
| 40 mM | 0.0539 mL | 0.2697 mL | 0.5395 mL | 1.3487 mL | |
| 50 mM | 0.0432 mL | 0.2158 mL | 0.4316 mL | 1.0790 mL | |
| 60 mM | 0.0360 mL | 0.1798 mL | 0.3597 mL | 0.8992 mL | |
| 80 mM | 0.0270 mL | 0.1349 mL | 0.2697 mL | 0.6744 mL | |
| 100 mM | 0.0216 mL | 0.1079 mL | 0.2158 mL | 0.5395 mL |
FM19G11 Related Classifications
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