GB1490
Based on 1 Customer Validation
GB1490 is an orally active galectin inhibitor with Kd values of 0.4 μM and 2.7 μM for galectin-1 and galectin-3, respectively.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit: 98.48%
- CAS. Nr.: 2172866-22-5
- Formel: C17H15Cl2FN4O4S2
- Molecular Weight:493.36
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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Biologische Aktivität
Beschreibung
IC50 & Target
[1]|
Galectin-1 0.4 μM (Kd) |
Galectin-3 2.7 μM (Kd) |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
>90 μM
Compound: 4c; GB1490
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Cytotoxicity against human A549 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
Cytotoxicity against human A549 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
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[PMID: 38059452] |
| Jurkat | IC50 |
2.3 μM
Compound: 4c; GB1490
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Inhibition of galectin-1 induced apoptosis in human Jurkat T cells assessed as reduction in caspase-3 activation incubated for 24 hrs by CellEvent caspase-3/7 green fluorescent detection reagent based imaging analysis
Inhibition of galectin-1 induced apoptosis in human Jurkat T cells assessed as reduction in caspase-3 activation incubated for 24 hrs by CellEvent caspase-3/7 green fluorescent detection reagent based imaging analysis
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[PMID: 38059452] |
| Jurkat | IC50 |
2.3 μM
Compound: GB1490
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Inhibition of apoptosis in galectin-1-induced human Jurkat cells measured after 16 hrs by caspase-3/7 green fluorescence based analysis
Inhibition of apoptosis in galectin-1-induced human Jurkat cells measured after 16 hrs by caspase-3/7 green fluorescence based analysis
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[PMID: 38804039] |
Chemical Information
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CAS. Nr. 2172866-22-5
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Appearance Solid
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Molecular Weight 493.36
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Formel C17H15Cl2FN4O4S2
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Color White to off-white
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SMILES
O[C@@H]1[C@@H](N2C=C(C3=NC=CS3)N=N2)[C@H]([C@H](O[C@@H]1SC4=CC(Cl)=C(C(Cl)=C4)F)CO)O
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 100 mg/mL (202.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (10.13 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (10.13 mM); Suspended solution
This protocol yields a suspended solution of ≥ 5 mg/mL (saturation unknown). Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.0269 mL | 10.1346 mL | 20.2692 mL | 50.6729 mL |
| 5 mM | 0.4054 mL | 2.0269 mL | 4.0538 mL | 10.1346 mL | |
| 10 mM | 0.2027 mL | 1.0135 mL | 2.0269 mL | 5.0673 mL | |
| 15 mM | 0.1351 mL | 0.6756 mL | 1.3513 mL | 3.3782 mL | |
| 20 mM | 0.1013 mL | 0.5067 mL | 1.0135 mL | 2.5336 mL | |
| 25 mM | 0.0811 mL | 0.4054 mL | 0.8108 mL | 2.0269 mL | |
| 30 mM | 0.0676 mL | 0.3378 mL | 0.6756 mL | 1.6891 mL | |
| 40 mM | 0.0507 mL | 0.2534 mL | 0.5067 mL | 1.2668 mL | |
| 50 mM | 0.0405 mL | 0.2027 mL | 0.4054 mL | 1.0135 mL | |
| 60 mM | 0.0338 mL | 0.1689 mL | 0.3378 mL | 0.8445 mL | |
| 80 mM | 0.0253 mL | 0.1267 mL | 0.2534 mL | 0.6334 mL | |
| 100 mM | 0.0203 mL | 0.1013 mL | 0.2027 mL | 0.5067 mL |
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