Art. -Nr.: HY-139214

IXA4

11 Cited Publications

Kundenbewertung

Based on 11 publication(s) in Google Scholar

IXA4 is a highly selective, non-toxic IRE1/XBP1s activator. IXA4 activates IRE1/XBP1s signaling without globally activating the unfolded protein response (UPR) or other stress-responsive signaling pathways (e.g., the heat shock response or oxidative stress response). IXA4 reduces secretion of APP through IRE1 activation.

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Reinheit: 98.92%
CAS. Nr.: 1185329-96-7
Formel: C₂₄H₂₈N₄O₄
Molecular Weight:436.50

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months

IXA4 Chemical Structure

CAS. Nr.: 1185329-96-7

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- In Vitro
Chemical Information
Publications (11)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

In Vitro

IXA4 (10 μM; 4 hours) selectively upregulates XBP1s mRNA, relative to genes regulated by ATF6 (e.g., BiP) or PERK (e.g., CHOP), in other cell lines including Huh7 and SHSY5Y cells^[1].
IXA4 (10μM; 18 hours) reduces Aβ levels 50% in conditioned media prepared on CHO7PA2 cells expressing the V717F APP (APPV717F) mutant^[1].
IXA4 rescues mitochondrial defects in SH-SY5Y cells expressing disease-relevant APP mutants. IXA4 (10μM; 4 hours) promotes adaptive IRE1/XBP1s signaling, but not RIDD, following 4 hrs of treatment in HEK293T cells^[1].
IXA4 also promotes selective transcriptional remodeling of ER proteostasis pathways, relative to cytosolic or mitochondrial pathways^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 1185329-96-7
Appearance Solid
Molecular Weight 436.50
Formel C₂₄H₂₈N₄O₄
Color White to off-white
SMILES

O=C(N(C)CCOC1=CC=C(C)C=C1)CN2N=CC(NC(CCOC3=CC=CC=C3)=O)=C2
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Publications (11)

All Flow CytometryCell Proliferation/Viability AssayBio/Physico-chemical AssayRT-PCRHistological Imaging/Staining

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Journal Impact Factor

Nat Commun

SEPTIN2 suppresses an IFN-γ-independent, proinflammatory macrophage activation pathway. [Abstract]

2023 Nov 17;14(1):7441. PMID: 37978190

IXA4 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Nov 17;14(1):7441. [Abstract]

PBMCs obtained from influenza patients with cytokine storm were pretreated with IXA4 (10 μM; 6 h). The expression of iNOS, CD80, and CD86 was detected. IXA4 significantly inhibited M1-like hyperpolarization in PBMCs.

IXA4 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Nov 17;14(1):7441. [Abstract]

IXA4 (10 μM; 6 h) effectively alleviated the outburst of proinflammatory cytokines in influenza patients.
Adv Sci (Weinh)

CEBPG-Mediated Palmitic Acid Adaptation of Cancer-Associated Fibroblasts Drives Metastasis of Oral Squamous Cell Carcinoma. [Abstract]

2026 Jun 2:e75875. PMID: 42227941
Free Radic Biol Med

TRIB2 promotes pulmonary artery smooth muscle cell proliferation through SERCA2 ubiquitination in pulmonary hypertension. [Abstract]

2025 Nov 8:243:414-433. PMID: 41213438
Ecotoxicol Environ Saf

Thallium-induced neurocardiotoxicity in zebrafish: Protective role of adaptive UPR and DNA repair. [Abstract]

2025 May 14:298:118321. PMID: 40373714

IXA4 purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2025 May 14:298:118321. [Abstract]

Survival rates and hatching rates of zebrafish embryos exposed to Tl (200 μg/L) with or without IXA4 (20 μM) treatments.

IXA4 purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2025 May 14:298:118321. [Abstract]

IXA4 (20 μM; 6-48 hpf) treatment significantly restored the expression of NER-related genes (xpc, rad23b, and ercc5) and markedly increased the expression of atm in zebrafish embryos.

IXA4 purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2025 May 14:298:118321. [Abstract]

The representative figures of H&E-stained sections of zebrafish midbrain, hindbrain, and heart transverse after being treated with Tl (800 μg/L) and Tl + IXA4 (20 μM) from 6 to 72 hpf. IXA4 offered protective benefits against the histological alterations induced by Tl in both cerebral and cardiac tissues.
Biochem Pharmacol

Ghrelin attenuates metabolic dysfunction-associated steatotic liver disease by activating glutamine metabolism via inhibition of the IRE1α-XBP-1 pathway. [Abstract]

2025 Jun 26:240:117089. PMID: 40581331
Cell Regen

The critical role of BMP signaling in gastric epithelial cell differentiation revealed by organoids. [Abstract]

2025 May 16;14(1):18. PMID: 40377813
Microbiologyopen

BvrR From Brucella abortus Induces Neuroinflammation Through IRE1-Mediated Activation of ATF2 and NF-κB. [Abstract]

2026 Feb;15(1):e70219. PMID: 41566528
Microb Pathog

BvrR of Brucella initiates microglial inflammation through the activation of the IRE1 pathway. [Abstract]

2026 Jan 21:108313. PMID: 41577152
Global Spine J

Needle-Knife Therapy Relieves Inflammation and Neuronal Apoptosis in Cervical Spondylotic Radiculopathy via IRE1α-XBP1 Signaling. [Abstract]

2026 Apr 16:21925682261443920. PMID: 41990753
Vet Microbiol

African swine fever virus maintains de novo global cellular protein synthesis and inhibits stress granules formation via dephosphorylating eIF2α. [Abstract]

2024 Mar:290:109988. PMID: 38244395
bioRxiv

2025 May 13.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (229.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.2910 mL

11.4548 mL

22.9095 mL

5 mM

0.4582 mL

2.2910 mL

4.5819 mL

10 mM

0.2291 mL

1.1455 mL

2.2910 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (5.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (5.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline

Solubility: 5 mg/mL (11.45 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (271 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Grandjean JMD, et al. Pharmacologic IRE1/XBP1s activation confers targeted ER proteostasis reprogramming. Nat Chem Biol. 2020;16(10):1052-1061. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.2910 mL	11.4548 mL	22.9095 mL	57.2738 mL
	5 mM	0.4582 mL	2.2910 mL	4.5819 mL	11.4548 mL
	10 mM	0.2291 mL	1.1455 mL	2.2910 mL	5.7274 mL
	15 mM	0.1527 mL	0.7637 mL	1.5273 mL	3.8183 mL
	20 mM	0.1145 mL	0.5727 mL	1.1455 mL	2.8637 mL
	25 mM	0.0916 mL	0.4582 mL	0.9164 mL	2.2910 mL
	30 mM	0.0764 mL	0.3818 mL	0.7637 mL	1.9091 mL
	40 mM	0.0573 mL	0.2864 mL	0.5727 mL	1.4318 mL
	50 mM	0.0458 mL	0.2291 mL	0.4582 mL	1.1455 mL
	60 mM	0.0382 mL	0.1909 mL	0.3818 mL	0.9546 mL
	80 mM	0.0286 mL	0.1432 mL	0.2864 mL	0.7159 mL
	100 mM	0.0229 mL	0.1145 mL	0.2291 mL	0.5727 mL

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IXA4

Cell Cycle/DNA Damage Compound Library

Kinase Inhibitor Library

Anti-Aging Compound Library

Oxygen Sensing Compound Library

Endoplasmic Reticulum Stress Compound Library

Anti-Parkinson's Disease Compound Library

Neurodegenerative Disease-related Compound Library

Heterocyclic Compound Library

Highly Selective Inhibitors Library

Bioactive Compound Library Max

Anti-Aging Compound Library Mini

MCE Bioactive Compound Library

Bioactive Compound Library

Protein Kinase Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Publications Citing Use of MedChemExpress (MCE) IXA4

Biologische Aktivität

Chemical Information

Publications (11)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

IXA4

Cell Cycle/DNA Damage Compound Library

Kinase Inhibitor Library

Anti-Aging Compound Library

Oxygen Sensing Compound Library

Endoplasmic Reticulum Stress Compound Library

Anti-Parkinson's Disease Compound Library

Neurodegenerative Disease-related Compound Library

Heterocyclic Compound Library

Highly Selective Inhibitors Library

Bioactive Compound Library Max

Anti-Aging Compound Library Mini

MCE Bioactive Compound Library

Bioactive Compound Library

Protein Kinase Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Publications Citing Use of MedChemExpress (MCE) IXA4

Biologische Aktivität

Chemical Information

Publications (11)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

IXA4 Related Classifications

HY-139214 Related Classifications